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SMEK_DICDI
ID   SMEK_DICDI              Reviewed;        1046 AA.
AC   Q54I18;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Suppressor of Mek1;
DE            Short=SMEK;
GN   Name=smkA; ORFNames=DDB_G0289067;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND DEVELOPMENTAL STAGE.
RX   PubMed=16107728; DOI=10.1128/mcb.25.17.7839-7853.2005;
RA   Mendoza M.C., Du F., Iranfar N., Tang N., Ma H., Loomis W.F., Firtel R.A.;
RT   "Loss of SMEK, a novel, conserved protein, suppresses MEK1 null cell
RT   polarity, chemotaxis, and gene expression defects.";
RL   Mol. Cell. Biol. 25:7839-7853(2005).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH PPP4C.
RX   PubMed=17353263; DOI=10.1128/mcb.02194-06;
RA   Mendoza M.C., Booth E.O., Shaulsky G., Firtel R.A.;
RT   "MEK1 and protein phosphatase 4 coordinate Dictyostelium development and
RT   chemotaxis.";
RL   Mol. Cell. Biol. 27:3817-3827(2007).
CC   -!- FUNCTION: Suppresses MEK1 null cell polarity, chemotaxis, and gene
CC       expression defects. Required for proper cytokinesis during vegetative
CC       growth, timely exit from the mound stage during development, and myosin
CC       II assembly. May be a regulatory subunit of serine/threonine-protein
CC       phosphatase 4 (PP4) and may control localization of PP4 to the nucleus.
CC       Involved in the regulation of some ppp4c functions, such as
CC       developmental progression, chemotaxis, expression of stress response
CC       genes and cell movement. {ECO:0000269|PubMed:16107728,
CC       ECO:0000269|PubMed:17353263}.
CC   -!- SUBUNIT: Interacts with ppp4c. {ECO:0000269|PubMed:17353263}.
CC   -!- INTERACTION:
CC       Q54I18; Q9Y0B7: ppp4c; NbExp=2; IntAct=EBI-2015890, EBI-2015876;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC       {ECO:0000269|PubMed:16107728}. Nucleus {ECO:0000269|PubMed:16107728}.
CC       Note=Localizes to the cell cortex in vegetative cells but translocates
CC       to the nucleus during starvation and development (PubMed:16107728).
CC   -!- DEVELOPMENTAL STAGE: Levels are constant throughout development.
CC       {ECO:0000269|PubMed:16107728}.
CC   -!- DOMAIN: The EVH1/WH1 domain is necessary for cortical localization.
CC       {ECO:0000269|PubMed:16107728}.
CC   -!- SIMILARITY: Belongs to the SMEK family. {ECO:0000305}.
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DR   EMBL; AAFI02000129; EAL62912.1; -; Genomic_DNA.
DR   RefSeq; XP_636416.1; XM_631324.1.
DR   AlphaFoldDB; Q54I18; -.
DR   SMR; Q54I18; -.
DR   IntAct; Q54I18; 1.
DR   STRING; 44689.DDB0232046; -.
DR   PaxDb; Q54I18; -.
DR   PRIDE; Q54I18; -.
DR   EnsemblProtists; EAL62912; EAL62912; DDB_G0289067.
DR   GeneID; 8626945; -.
DR   KEGG; ddi:DDB_G0289067; -.
DR   dictyBase; DDB_G0289067; smkA.
DR   eggNOG; KOG2175; Eukaryota.
DR   HOGENOM; CLU_004909_3_0_1; -.
DR   InParanoid; Q54I18; -.
DR   OMA; MMRGYML; -.
DR   PhylomeDB; Q54I18; -.
DR   PRO; PR:Q54I18; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR   GO; GO:0030289; C:protein phosphatase 4 complex; IDA:dictyBase.
DR   GO; GO:0072542; F:protein phosphatase activator activity; IBA:GO_Central.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IBA:GO_Central.
DR   GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR   GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR   GO; GO:0007165; P:signal transduction; IGI:dictyBase.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR006887; DUF625.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   Pfam; PF04802; SMK-1; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..1046
FT                   /note="Suppressor of Mek1"
FT                   /id="PRO_0000254608"
FT   DOMAIN          1..101
FT                   /note="WH1"
FT   REGION          626..1046
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           1003..1022
FT                   /note="Nuclear localization signal"
FT   COMPBIAS        626..658
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        670..689
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        690..715
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        743..757
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        758..786
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        806..821
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        822..878
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        879..910
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        911..931
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        952..999
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1027..1046
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1046 AA;  119988 MW;  BE7680EFA773B572 CRC64;
     MEPLRKRVKV YQLDNSGKWD DKGTGHVSCI YVDALCAMGL IVRSESDNSV ILQTRLSAED
     IYQKQQDSLI VWTEPDSQLD LALSFQDSLG CQDIWENILQ YQNQRTGSCD SVDLDLPPVS
     INNLQTINEL LEASLPMLDK DKIINSIFKE DLVRSLLDLF DEIEKSGEGG VHLFQIFNIF
     KNLILFNDTS ILEVILSEDY LVRVMGALEY DPEISENNRI KHREFLNQQV VFKQVIKFPS
     KSLIGTIHQT FRIQYLKDVV LPRVLDDVTF SSLNSLIYFN NIDIVSQIQN DSDFLENLFS
     EIQKSEKNSE ERKDLILFLQ DLCNLAKGLQ IQSKSTFFTV VVSLGLFKTL SAILDDENVQ
     TRVSCTEIVL STLLHDPEIL RSYLCSPTSG NSKFLVQLIN LFITDKDIGV KNQIVEIIKT
     LLEADSYDSS DFFRLFYDKG IDLLVSPLNE VYKGEPTIPG DPSSNLDSFV LYNIMELVIY
     CIKHHCYRIK HFIVEEGIAK KILRYTNPTG SGGGGGGGGN SERYLILGSI RFFRSMVNMK
     DDLYNQHIIQ ENLFEPIIEV FKSNISRYNL LNSAIIELFQ YIYKENIRDL IVYLVERYRE
     LFESVTYTDV LKQLILKYEQ IKDSSFESPE TSCNNNDSSS NDIDSKPIIG NNKINHNYQR
     TQREIDEEEE EAYFNRDDDS EDSDDEDELI PISINNNNNN NNNNKQICTN NENNMEKNDD
     NIEKDNENTN NGNGSSHIKI VDYEDEDDED DEINKSVESD DIVEKHEIID KNEKKDEIMK
     ENNDSDNDDN DNNDNDNDND NNSDIENKNH LNNNGNNENN ENNDDVQDKS NNKNNSDKIN
     EDEKIEKQDE MKENLEMEEI DEKVKEKQPK DIKKENQSQP DETVFNGKSN NSNNNNNNNN
     NNSNNQEIGD NRKTTPKRKL DYEKNESVVS KKIDKSNGPT SIDKDINGCD ESPNKKLNNN
     NSNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNQNDENEL SSASEEEEEQ LENGKHIKKF
     KRGKKDSNNS SNNSNNSSPT PSELHV
 
 
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