SMF_ECOLI
ID SMF_ECOLI Reviewed; 374 AA.
AC P30852; P76682; P76683; Q2M6V0; Q47136;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein Smf;
GN Name=smf; OrderedLocusNames=b4473, JW5708;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / K37;
RX PubMed=8432722; DOI=10.1128/jb.175.4.993-1000.1993;
RA Meinnel T., Guillon J.-M., Mechulam Y., Blanquet S.;
RT "The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet)
RT formyltransferase, escapes metabolic control.";
RL J. Bacteriol. 175:993-1000(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8112305; DOI=10.1002/j.1460-2075.1994.tb06335.x;
RA Mazel D., Pochet S., Marliere P.;
RT "Genetic characterization of polypeptide deformylase, a distinctive enzyme
RT of eubacterial translation.";
RL EMBO J. 13:914-923(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP SEQUENCE REVISION.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12, KA797, and PC4020;
RX PubMed=16978360; DOI=10.1111/j.1574-6968.2006.00423.x;
RA Smeets L.C., Becker S.C., Barcak G.J., Vandenbroucke-Grauls C.M.,
RA Bitter W., Goosen N.;
RT "Functional characterization of the competence protein DprA/Smf in
RT Escherichia coli.";
RL FEMS Microbiol. Lett. 263:223-228(2006).
CC -!- FUNCTION: Partially complements natural chromosomal DNA transformation
CC defect of an H.influenzae dprA disruption mutant (PubMed:16978360). May
CC help load RecA onto ssDNA (By similarity).
CC {ECO:0000250|UniProtKB:Q8DPI7, ECO:0000269|PubMed:16978360}.
CC -!- DISRUPTION PHENOTYPE: No visible effect on transformation or growth in
CC the presence or absence of mitomycin C (in strain KA797), on plasmid
CC conjugation (in strain PC4020), on nucleotide excision repair, survival
CC of UV damage or DNA recombination (PubMed:16978360).
CC {ECO:0000269|PubMed:16978360}.
CC -!- SIMILARITY: Belongs to the DprA/Smf family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA58082.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA58083.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X65946; CAA46763.1; -; Genomic_DNA.
DR EMBL; X77091; CAA54366.1; -; Genomic_DNA.
DR EMBL; X77800; CAA54827.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58083.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18997; AAA58082.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAT48176.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78006.1; -; Genomic_DNA.
DR RefSeq; WP_000228551.1; NZ_SSUW01000045.1.
DR RefSeq; YP_026211.1; NC_000913.3.
DR AlphaFoldDB; P30852; -.
DR SMR; P30852; -.
DR BioGRID; 4263397; 17.
DR STRING; 511145.b4473; -.
DR PaxDb; P30852; -.
DR PRIDE; P30852; -.
DR EnsemblBacteria; AAT48176; AAT48176; b4473.
DR EnsemblBacteria; BAE78006; BAE78006; BAE78006.
DR GeneID; 2847708; -.
DR KEGG; ecj:JW5708; -.
DR KEGG; eco:b4473; -.
DR PATRIC; fig|511145.12.peg.3379; -.
DR EchoBASE; EB1561; -.
DR eggNOG; COG0758; Bacteria.
DR HOGENOM; CLU_029601_1_1_6; -.
DR InParanoid; P30852; -.
DR OMA; SEMPFGW; -.
DR PhylomeDB; P30852; -.
DR BioCyc; EcoCyc:EG11604-MON; -.
DR PRO; PR:P30852; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009294; P:DNA-mediated transformation; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003488; DprA.
DR InterPro; IPR041614; DprA_WH.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43022; PTHR43022; 1.
DR Pfam; PF02481; DNA_processg_A; 1.
DR Pfam; PF17782; DprA_WH; 1.
DR TIGRFAMs; TIGR00732; dprA; 1.
PE 3: Inferred from homology;
KW Reference proteome.
FT CHAIN 1..374
FT /note="Protein Smf"
FT /id="PRO_0000209157"
FT CONFLICT 240
FT /note="A -> R (in Ref. 2; CAA54827)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 374 AA; 40955 MW; 478F522ECB71FA41 CRC64;
MVDTEIWLRL MSISSLYGDD MVRIAHWVAK QSHIDAVVLQ QTGLTLRQAQ RFLSFPRKSI
ESSLCWLEQP NHHLIPADSE FYPPQLLATT DYPGALFVEG ELHALHSFQL AVVGSRAHSW
YGERWGRLFC ETLATRGVTI TSGLARGIDG VAHKAALQVN GVSIAVLGNG LNTIHPRRHA
RLAASLLEQG GALVSEFPLD VPPLAYNFPR RNRIISGLSK GVLVVEAALR SGSLVTARCA
LEQGREVFAL PGPIGNPGSE GPHWLIKQGA ILVTEPEEIL ENLQFGLHWL PDAPENSFYS
PDQQDVALPF PELLANVGDE VTPVDVVAER AGQPVPEVVT QLLELELAGW IAAVPGGYVR
LRRACHVRRT NVFV
