SMG7_MOUSE
ID SMG7_MOUSE Reviewed; 1138 AA.
AC Q5RJH6; Q63ZW5; Q6ZQF3;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Nonsense-mediated mRNA decay factor SMG7 {ECO:0000312|MGI:MGI:2682334};
DE AltName: Full=SMG-7 homolog {ECO:0000250|UniProtKB:Q92540};
GN Name=Smg7 {ECO:0000312|MGI:MGI:2682334};
GN Synonyms=Est1c {ECO:0000250|UniProtKB:Q92540},
GN Kiaa0250 {ECO:0000312|MGI:MGI:2682334};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Embryonic germ cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a role in nonsense-mediated mRNA decay. Recruits UPF1
CC to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to
CC provide a link to the mRNA degradation machinery involving
CC exonucleolytic pathways, and to serve as an adapter for UPF1 to protein
CC phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of a complex that contains SMG5, SMG7, PPP2CA, a short
CC isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and
CC phosphorylated UPF1 (By similarity). Interacts with DHX34; the
CC interaction is RNA-independent (By similarity).
CC {ECO:0000250|UniProtKB:Q92540}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q92540}. Nucleus
CC {ECO:0000250|UniProtKB:Q92540}. Note=Predominantly cytoplasmic, and
CC nuclear. Shuttles between nucleus and cytoplasm.
CC {ECO:0000250|UniProtKB:Q92540}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5RJH6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5RJH6-2; Sequence=VSP_016577;
CC Name=3;
CC IsoId=Q5RJH6-3; Sequence=VSP_016578, VSP_016579;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC97911.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK129101; BAC97911.1; ALT_INIT; mRNA.
DR EMBL; BC082789; AAH82789.1; -; mRNA.
DR EMBL; BC086651; AAH86651.1; -; mRNA.
DR CCDS; CCDS35740.1; -. [Q5RJH6-3]
DR CCDS; CCDS48394.1; -. [Q5RJH6-2]
DR CCDS; CCDS48395.1; -. [Q5RJH6-1]
DR RefSeq; NP_001005507.1; NM_001005507.2. [Q5RJH6-3]
DR RefSeq; NP_001153728.1; NM_001160256.1. [Q5RJH6-1]
DR RefSeq; NP_001153729.1; NM_001160257.1. [Q5RJH6-2]
DR RefSeq; XP_006529515.1; XM_006529452.3.
DR AlphaFoldDB; Q5RJH6; -.
DR SMR; Q5RJH6; -.
DR BioGRID; 230520; 6.
DR STRING; 10090.ENSMUSP00000041241; -.
DR iPTMnet; Q5RJH6; -.
DR PhosphoSitePlus; Q5RJH6; -.
DR EPD; Q5RJH6; -.
DR MaxQB; Q5RJH6; -.
DR PaxDb; Q5RJH6; -.
DR PeptideAtlas; Q5RJH6; -.
DR PRIDE; Q5RJH6; -.
DR ProteomicsDB; 261259; -. [Q5RJH6-1]
DR ProteomicsDB; 261260; -. [Q5RJH6-2]
DR ProteomicsDB; 261261; -. [Q5RJH6-3]
DR Antibodypedia; 34445; 110 antibodies from 23 providers.
DR DNASU; 226517; -.
DR Ensembl; ENSMUST00000043560; ENSMUSP00000041241; ENSMUSG00000042772. [Q5RJH6-2]
DR Ensembl; ENSMUST00000073441; ENSMUSP00000073144; ENSMUSG00000042772. [Q5RJH6-3]
DR Ensembl; ENSMUST00000111836; ENSMUSP00000107467; ENSMUSG00000042772. [Q5RJH6-1]
DR GeneID; 226517; -.
DR KEGG; mmu:226517; -.
DR UCSC; uc007czn.1; mouse. [Q5RJH6-2]
DR UCSC; uc007czo.2; mouse. [Q5RJH6-3]
DR UCSC; uc007czp.2; mouse. [Q5RJH6-1]
DR CTD; 9887; -.
DR MGI; MGI:2682334; Smg7.
DR VEuPathDB; HostDB:ENSMUSG00000042772; -.
DR eggNOG; KOG2162; Eukaryota.
DR GeneTree; ENSGT00940000158333; -.
DR HOGENOM; CLU_009299_0_0_1; -.
DR InParanoid; Q5RJH6; -.
DR OMA; TWAGHGP; -.
DR OrthoDB; 556396at2759; -.
DR PhylomeDB; Q5RJH6; -.
DR TreeFam; TF327119; -.
DR Reactome; R-MMU-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 226517; 20 hits in 76 CRISPR screens.
DR ChiTaRS; Smg7; mouse.
DR PRO; PR:Q5RJH6; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q5RJH6; protein.
DR Bgee; ENSMUSG00000042772; Expressed in embryonic post-anal tail and 224 other tissues.
DR Genevisible; Q5RJH6; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:HGNC-UCL.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0045111; C:intermediate filament cytoskeleton; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:HGNC-UCL.
DR GO; GO:0005697; C:telomerase holoenzyme complex; IBA:GO_Central.
DR GO; GO:0051721; F:protein phosphatase 2A binding; ISS:HGNC-UCL.
DR GO; GO:0070034; F:telomerase RNA binding; IBA:GO_Central.
DR GO; GO:0042162; F:telomeric DNA binding; ISO:MGI.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR018834; DNA/RNA-bd_Est1-type.
DR InterPro; IPR045153; Est1/Ebs1-like.
DR InterPro; IPR019458; Est1_N.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR15696; PTHR15696; 1.
DR Pfam; PF10374; EST1; 1.
DR Pfam; PF10373; EST1_DNA_bind; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Alternative splicing; Cytoplasm; Nonsense-mediated mRNA decay;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT CHAIN 2..1138
FT /note="Nonsense-mediated mRNA decay factor SMG7"
FT /id="PRO_0000076325"
FT REPEAT 152..185
FT /note="TPR 1"
FT REPEAT 187..219
FT /note="TPR 2"
FT REGION 515..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 649..745
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 838..871
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 990..1090
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1106..1138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 544..578
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 580..599
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..682
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..697
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 698..726
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 990..1031
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1061..1088
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT MOD_RES 519
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT MOD_RES 575
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT MOD_RES 732
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT MOD_RES 848
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92540"
FT VAR_SEQ 1..9
FT /note="MSLQSAQYL -> MRTENLKSEEHLKSSNI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_016577"
FT VAR_SEQ 566
FT /note="V -> VRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQV
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_016578"
FT VAR_SEQ 866..915
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_016579"
FT CONFLICT 552
FT /note="P -> S (in Ref. 1; BAC97911)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1138 AA; 126841 MW; 330576E241E35AD4 CRC64;
MSLQSAQYLR QAEVLKAEMT DSKLGPAEVW TSRQALQDLY QKMLVTDLEY ALDKKVEQDL
WNHAFKNQIT TLQGQAKNRA NPNRSEVQAN LSLFLEAASG FYTQLLQELC TVFNVDLPCR
VKSSQLGIIS NKQTHSSTIV KPQSSSCSYI CQHCLVHLGD IARYRNQTSQ AESYYRHAAQ
LVPSNGQPYN QLAILASSKG DHLTTIFYYC RSIAVKFPFP AASTNLQKAL SKALESRDEL
KTKWGVSDFI KAFIKFHGHV YLSKSLEKLS PLREKLEEQF KRLLFQKAFN SQQLVHVTVI
NLFQLHHLRD FSNETEQHSY SQDEQLCWTQ LLALFMSFLG ILCKCPLQND SQESNNAYPL
PAVKVSMDWL RLRPRVFQEA VVDERQYIWP WLISLLNSFH PREDDLSNTN ATPLPEEFEL
QGFLALRPSF RNLDFSKGHQ GITGDKEGQQ RRIRQQRLIS IGKWIADNQP RLIQCENEVG
KLLFITEIPE LILEDPSEAK ENLILQETSV VESLATDGSP GLKSVLSTGR NPSNSCDSGE
KPVVTFKENI KPREVNQGRS FPPKEVKSQT ELRKTPVSEA RKTPVTQTPS QTSNSQFIPI
HHPGAFPPLP SRPGFPPPTY VIPPPVAFSM GSGYTFPAGV SVPGTFLQST AHSPAGNQVQ
AGKQSHIPYS QQRPSGPGPM NQGPQQSQPP SQPPLTSLPA QPTAQSTSQL QVQALAQQQQ
SPTKVIPALG KSPPHHSGFQ QYQQADASKQ LWNPPQVQSP LGKIMPVKQS YYLQTQDPIK
LFEPSLQPPV IQQQPLEKKM KPFPMEPYNH NPSEVKVPEF YWDSSYSMAD NRAVMAQQPN
MDRRSKRSPG VFRPEQDPVP RMPFEDPKSS PLLPPDLLKS LAALEEEEEL IFSNPPDLYP
ALLGPLASLP GRSLFKSLLE KPSELMSHSS SFLSLTGFSV NQERYPNSSM FNEVYGKNLT
TSSKAELNPS VASQETSLYS LFEGTPWSPS LPASSDHSTP ASQSPHSSNP SSLPSSPPTH
NHNSAPFSNF GPIGTPDNRD RRPADRWKTD KPAMGGFGVD YLSATSSSES SWHQASTPSG
TWTGHGPSME DSSAVLMESL KSIWSSSMMH PGPSALEQLL MQQKQKQQRG QGAMNPPH
