SMG8_CAEEL
ID SMG8_CAEEL Reviewed; 873 AA.
AC O62301;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 3.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Nonsense-mediated mRNA decay factor SMG8 {ECO:0000250|UniProtKB:Q8ND04};
DE AltName: Full=Suppressor with morphogenetic effect on genitalia protein 8;
GN Name=smg-8; ORFNames=K04B12.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19417104; DOI=10.1101/gad.1767209;
RA Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y.,
RA Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H.,
RA Anderson P., Ohno S.;
RT "SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate
RT remodeling of the mRNA surveillance complex during nonsense-mediated mRNA
RT decay.";
RL Genes Dev. 23:1091-1105(2009).
CC -!- FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing
CC premature stop codons. Probable component of kinase complex containing
CC smg-1 and recruited to stalled ribosomes.
CC {ECO:0000269|PubMed:19417104}.
CC -!- DISRUPTION PHENOTYPE: Defects in nonsense-mediated mRNA decay (NMD).
CC {ECO:0000269|PubMed:19417104}.
CC -!- SIMILARITY: Belongs to the SMG8 family. {ECO:0000305}.
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DR EMBL; Z83232; CAB05756.3; -; Genomic_DNA.
DR RefSeq; NP_497003.3; NM_064602.4.
DR PDB; 5NKK; X-ray; 2.64 A; A/C/E=1-423.
DR PDB; 5NKM; X-ray; 2.49 A; A/C=1-423, E=1-419.
DR PDBsum; 5NKK; -.
DR PDBsum; 5NKM; -.
DR AlphaFoldDB; O62301; -.
DR SMR; O62301; -.
DR BioGRID; 40384; 1.
DR STRING; 6239.K04B12.3a; -.
DR EPD; O62301; -.
DR PaxDb; O62301; -.
DR PeptideAtlas; O62301; -.
DR EnsemblMetazoa; K04B12.3a.1; K04B12.3a.1; WBGene00010551.
DR GeneID; 175103; -.
DR KEGG; cel:CELE_K04B12.3; -.
DR UCSC; K04B12.3; c. elegans.
DR CTD; 175103; -.
DR WormBase; K04B12.3a; CE41824; WBGene00010551; smg-8.
DR eggNOG; KOG3692; Eukaryota.
DR HOGENOM; CLU_375675_0_0_1; -.
DR InParanoid; O62301; -.
DR OMA; HCTVKDS; -.
DR OrthoDB; 645776at2759; -.
DR PhylomeDB; O62301; -.
DR Reactome; R-CEL-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:O62301; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00010551; Expressed in germ line (C elegans) and 4 other tissues.
DR ExpressionAtlas; O62301; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; HDA:WormBase.
DR GO; GO:0055120; C:striated muscle dense body; HDA:WormBase.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:UniProtKB.
DR DisProt; DP03047; -.
DR InterPro; IPR028802; SMG8.
DR InterPro; IPR019354; SMG8/SMG9.
DR PANTHER; PTHR13091; PTHR13091; 1.
DR Pfam; PF10220; Smg8_Smg9; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Nonsense-mediated mRNA decay; Reference proteome.
FT CHAIN 1..873
FT /note="Nonsense-mediated mRNA decay factor SMG8"
FT /id="PRO_0000378169"
FT REGION 531..604
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..604
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 3..13
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 39..45
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 56..58
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5NKM"
FT TURN 68..71
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 83..91
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 98..117
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 121..131
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 134..160
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 175..178
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 211..228
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 232..234
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 244..246
FT /evidence="ECO:0007829|PDB:5NKM"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 290..299
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 308..317
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 319..323
FT /evidence="ECO:0007829|PDB:5NKM"
FT TURN 324..326
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 334..354
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 384..398
FT /evidence="ECO:0007829|PDB:5NKM"
FT HELIX 403..417
FT /evidence="ECO:0007829|PDB:5NKM"
SQ SEQUENCE 873 AA; 99970 MW; 179C81DDE3731776 CRC64;
MDIAKWVEHA RTCYSTQLDT KIKVIGVIGK DYPDHGKGDN INCYLRENVF PVAATEDETC
TIRGHFSEDD QILFLVMNGV DDVANIRKCL KSNPKSNYFD AMAESECQQI RMLHFLFISC
HFIIIFEQTS RIDLELMRFL KKVNSARIQL RKKINQRLVA SDLRDVSFNN RILSSAESEG
RMVVPRLLIA FQRNNIRPDV NPGKKLQREL YEKLEKNLDN QFSDILKLYD LIDCGASSLC
QLNETIPVVH LLNPKIVKRD IIGEMFEILM ADAENTKISG NAGTLPSNNS FVKFLEDNFR
SEKNEISLEN VIELMNCLQC VLDGDLEEKH EKTAIQTFIK RIQNDHMEEA RRLYTNAQRP
GERRGADRFK DSEKPVKIRS KEEHLMRFNE ATHYIDSVVG VNSREALSQL QAQCNEMWQS
DMRACESVSM MGRSCVRKIH PTFGDQTAPE HRWTAHDASN TMISTCVCGR KQLIRPEPFS
VKEANSDFYD HPDFKCCRRL WRYQFQLYQE DSEEKDDIMW ADRESNSLRA AKKMAQREDE
LAEEDTDLDI PESLLDPDST SPSDEDDVRV RTMSSSESSS QESDAYLRPT SRRDESMASK
TERELTIDHV KRMQKLELAG KSEDFLITVP NSMTTGKLPI FPSWHLTSLG DSSIYSHGAG
LKNQPNFKIG GDYLSPVVVL LDVNFDVWNR DLNKIRSEDF SRKCGKDNKD DSARVKLFVG
FEYECSRGHR FFVDYNGEPL IYSKGSNVIR ESAHRSSLGN VLQADLPIRR PCTCRKLPLQ
SAQLQKVHVV TPKAPVHITI DPKVLIPTHE GVYGTGQEPL ELHHSKYYIL HLPTVYSGPS
GAWMPGEFNP EKQGVWMGGA LKVAYKPVMS FKW
