SMG8_DROME
ID SMG8_DROME Reviewed; 944 AA.
AC Q9VKQ6; B5RIT4; Q8T4F6;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Nonsense-mediated mRNA decay factor SMG8 {ECO:0000250|UniProtKB:Q8ND04};
DE AltName: Full=Protein smg-8 homolog;
GN ORFNames=CG6729;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing
CC premature stop codons. Probable component of kinase complex containing
CC nonC and recruited to stalled ribosomes (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SMG8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACH92273.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF53005.1; -; Genomic_DNA.
DR EMBL; AY084211; AAL89949.1; -; mRNA.
DR EMBL; BT044208; ACH92273.1; ALT_INIT; mRNA.
DR RefSeq; NP_609449.1; NM_135605.3.
DR AlphaFoldDB; Q9VKQ6; -.
DR SMR; Q9VKQ6; -.
DR STRING; 7227.FBpp0079708; -.
DR PaxDb; Q9VKQ6; -.
DR DNASU; 34484; -.
DR EnsemblMetazoa; FBtr0080119; FBpp0079708; FBgn0032296.
DR GeneID; 34484; -.
DR KEGG; dme:Dmel_CG6729; -.
DR UCSC; CG6729-RA; d. melanogaster.
DR FlyBase; FBgn0032296; CG6729.
DR VEuPathDB; VectorBase:FBgn0032296; -.
DR eggNOG; KOG3692; Eukaryota.
DR GeneTree; ENSGT00390000018533; -.
DR HOGENOM; CLU_008116_0_0_1; -.
DR InParanoid; Q9VKQ6; -.
DR OMA; HCTVKDS; -.
DR OrthoDB; 645776at2759; -.
DR PhylomeDB; Q9VKQ6; -.
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 34484; 0 hits in 1 CRISPR screen.
DR ChiTaRS; CG6729; fly.
DR GenomeRNAi; 34484; -.
DR PRO; PR:Q9VKQ6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032296; Expressed in adult Malpighian tubule (Drosophila) and 23 other tissues.
DR Genevisible; Q9VKQ6; DM.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
DR InterPro; IPR028802; SMG8.
DR InterPro; IPR019354; SMG8/SMG9.
DR PANTHER; PTHR13091; PTHR13091; 1.
DR Pfam; PF10220; Smg8_Smg9; 1.
PE 2: Evidence at transcript level;
KW Nonsense-mediated mRNA decay; Reference proteome.
FT CHAIN 1..944
FT /note="Nonsense-mediated mRNA decay factor SMG8"
FT /id="PRO_0000304977"
FT REGION 559..601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 629..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..587
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 477
FT /note="L -> Q (in Ref. 3; AAL89949)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 944 AA; 106828 MW; E02D6405B35645BF CRC64;
MLDDYYTWTY PDIPENVAQE LLQLNGSLVV VGIVGRSDCD QANKMVAFGM EPPSEHTPKD
GQMQCYYKPG TSILLLHFES TYDAEIAGQM IDVCIEDVDT PFDIDSFFEG IRCRFVRMML
LALHVCHIVV YVETGQTFDP TLITVFQLAK FAREQHLMQF LPQMLRETPA ARMSERTRLC
TPRILFLFEN FPSDEPKTRE CVSTYEFQME DCIYELLRHH NIVTNSSSNS LVALPNNKQF
VFFNAHEELR EDTLMKAVEC LNETMYKPDL KEEEEDLEIL ALAPFDGFVK PFALPVDEKE
WDNQQYKKDH TVWNFLERHV QDALMGCFEA GSFKQHAQQG TFQLLNSKEW HDCMATMHTL
LVENTKDPNL ETSNEEYKNF LKNFDESLNY EKKFWAHLCE LGLKKGIAAY KNAAPENYGS
ATHRQLLAEA TLAFEEEGRG PQAQAALAKL ASICHKHWQD GRQQCEQLSL RSHPCTLPKK
VPHEKHNSGV IHISSCNCGR TQGRREDPFN LRQANYEFYE HIAQMCNLCV KVKQYQFPIF
EPSVSDYRAA AFEAAFPLLN NGKSGAPQDE DAEEDEAEEE EGQEQEQPTE EQLQNTASNC
CSQPLSPTFG SDLNMSIAGF GASLKESQAS SEQLLNSEQN TTSSGTSSAD TDNELVVELQ
EPAKKEARED AGPAHAVSTS TTEYLPGLVH TVSNFGLLPL FPSWSLACVG PSSIYSHNTG
LQEHFQSGFL SGANFLLPWD VQLRLVHAPK QQYQQQHHGK KQQRWKKQGD RLSLKIFVGM
EYECSRGHRF MMCAPDRVLR GGADIERETC SMVVHNNMPL YYPCPCRSQN NFLAQLMRIH
VVTPKAPVNI IVDPKVCVGR YTFTMGSIVP PRLSQSAYWI IRLPYVYQGD DVLIAPPEQL
DPGYPLTGGY LLPGMFGVVE TDPTLDLNEP DKMGASAAGN FTRI
