SMG8_MOUSE
ID SMG8_MOUSE Reviewed; 991 AA.
AC Q8VE18; B2KGQ4; Q8BS62; Q8BSP7; Q9DBW6;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Nonsense-mediated mRNA decay factor SMG8 {ECO:0000312|MGI:MGI:1921383};
DE AltName: Full=Protein smg-8 homolog;
GN Name=Smg8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Forelimb, and Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Liver, Pancreas, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-898, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing
CC premature stop codons. Is recruited by release factors to stalled
CC ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase
CC complex) and, in the SMG1C complex, is required to mediate the
CC recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1
CC kinase activity until the ribosome:SURF complex locates the exon
CC junction complex (EJC). Acts as a regulator of kinase activity (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the SMG1C complex composed of SMG1, SMG8 and
CC SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large
CC conformational change in the SMG1 C-terminal head domain containing the
CC catalytic domain. Forms heterodimers with SMG9; this assembly form may
CC represent a SMG1C intermediate form (By similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated by SMG1. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SMG8 family. {ECO:0000305}.
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DR EMBL; AK004714; BAB23498.2; -; mRNA.
DR EMBL; AK031106; BAC27255.1; -; mRNA.
DR EMBL; AK035089; BAC28941.1; -; mRNA.
DR EMBL; AL713917; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU407131; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC020005; AAH20005.1; -; mRNA.
DR CCDS; CCDS25207.1; -.
DR RefSeq; NP_077224.1; NM_024262.1.
DR AlphaFoldDB; Q8VE18; -.
DR SMR; Q8VE18; -.
DR BioGRID; 216516; 1.
DR STRING; 10090.ENSMUSP00000020801; -.
DR iPTMnet; Q8VE18; -.
DR PhosphoSitePlus; Q8VE18; -.
DR EPD; Q8VE18; -.
DR jPOST; Q8VE18; -.
DR MaxQB; Q8VE18; -.
DR PaxDb; Q8VE18; -.
DR PeptideAtlas; Q8VE18; -.
DR PRIDE; Q8VE18; -.
DR ProteomicsDB; 261262; -.
DR Antibodypedia; 31075; 55 antibodies from 18 providers.
DR DNASU; 74133; -.
DR Ensembl; ENSMUST00000020801; ENSMUSP00000020801; ENSMUSG00000020495.
DR GeneID; 74133; -.
DR KEGG; mmu:74133; -.
DR UCSC; uc007ktg.1; mouse.
DR CTD; 55181; -.
DR MGI; MGI:1921383; Smg8.
DR VEuPathDB; HostDB:ENSMUSG00000020495; -.
DR eggNOG; KOG3692; Eukaryota.
DR GeneTree; ENSGT00390000018533; -.
DR HOGENOM; CLU_008116_0_0_1; -.
DR InParanoid; Q8VE18; -.
DR OMA; HCTVKDS; -.
DR OrthoDB; 645776at2759; -.
DR PhylomeDB; Q8VE18; -.
DR TreeFam; TF323445; -.
DR Reactome; R-MMU-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 74133; 13 hits in 74 CRISPR screens.
DR ChiTaRS; Smg8; mouse.
DR PRO; PR:Q8VE18; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8VE18; protein.
DR Bgee; ENSMUSG00000020495; Expressed in secondary oocyte and 184 other tissues.
DR ExpressionAtlas; Q8VE18; baseline and differential.
DR Genevisible; Q8VE18; MM.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
DR GO; GO:0045859; P:regulation of protein kinase activity; ISS:UniProtKB.
DR InterPro; IPR028802; SMG8.
DR InterPro; IPR019354; SMG8/SMG9.
DR PANTHER; PTHR13091; PTHR13091; 1.
DR Pfam; PF10220; Smg8_Smg9; 1.
PE 1: Evidence at protein level;
KW Methylation; Nonsense-mediated mRNA decay; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..991
FT /note="Nonsense-mediated mRNA decay factor SMG8"
FT /id="PRO_0000304975"
FT REGION 18..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..738
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 655..669
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 671..685
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 686..709
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 724..738
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND04"
FT MOD_RES 469
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND04"
FT MOD_RES 668
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND04"
FT MOD_RES 742
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND04"
FT MOD_RES 895
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND04"
FT MOD_RES 898
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT CONFLICT 222
FT /note="T -> N (in Ref. 1; BAC27255)"
FT /evidence="ECO:0000305"
FT CONFLICT 829
FT /note="S -> R (in Ref. 1; BAC27255)"
FT /evidence="ECO:0000305"
FT CONFLICT 845
FT /note="A -> D (in Ref. 1; BAC27255)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 991 AA; 109667 MW; 14A43C60C8054413 CRC64;
MAGPVSLREL LMGASAWLGS ESPGGPSAEG GGNAAGTPEP PWREDEICVV GIFGKTALRL
NSEKFSLVNT VCDRQVFPLF HHQDPGDPGT GIKTKAVSVG EAGGAGDPGA AAGDSLRGGM
ATAEGNRAEP GPQDFSLLQA YYNQESKVLY LILTSICDNS QLLRACRALQ SGEAGGGLSL
PHAETHEFWK HQEKLQCLSL LYLFSVCHIL LLVHPTCSFD ITYDRVFRAL DGLRQKVLPL
LKTAIKDCPV GKDWKLNCRP CPPRLLFLFQ LNGALKVEPP RSQDTAHPDK PKKHSPKRRL
QHALEDQIYR IFRKSRVLTN QSINCLFTVP ANQAFVYIVP GSQEEDPIGM LLDQLRSHCT
VKDPESLLVP APLSGPRRYQ AMRQHSRQQL SFHIDSSTSS SSGQLVDFTL REFLWQHVEL
VLSKKGFDDS VGRNPQPSHF ELPTYQKWIS AAAKLYEVAI DGKEEDLGSP TGELTSKILS
SIKVLEGFLD IDTKFSENRC QKALPMAHSA YQSNLPHNYT MTVHKNQLAQ ALRVYSQHAR
GPAFHKYAMQ LHEDCYKFWS NGHQLCEERS LTDQHCVHKF HSLPKSGEKP EADRNPPVLY
HNSRARSTGA CNCGRKQAPR DDPFDIKAAN YDFYQLLEEK CCGKLDHINF PVFEPSTPDP
APAKNEPSPA PPDSDAEKLK EKEPQTQGES TSLSLALSLG QSTDSLGTYP ADPQAGGDNP
EVHGQGEGKS EKRPNLVDRQ ASTVEYLPGM LHSNCPKGLL PKFSSWSLVK LGPAKSYNFH
TGLDQQGFVP GTNYLMPWDI VIRTRAEDEG DLDTNSWPAP NKAIPGKRSA VVMGRGRRRD
DIARAFVGFE YEDSRGRRFM CSGPDKVMKV MGSGPKESAL KALNSDMPLY ILSSSQGRGL
KPHYAQLMRL FVVVPDAPLQ IILMPQVQPG PPPCPVFYPE KQEITLPPDG LWVLRFPYAY
VTERGPCFPP KENVQLMSYK VLRGVLKAVT Q
