ID   SMG8_XENTR              Reviewed;         915 AA.
AC   Q0VA04;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Nonsense-mediated mRNA decay factor SMG8 {ECO:0000250|UniProtKB:Q8ND04};
DE   AltName: Full=Protein smg-8 homolog;
GN   Name=smg8;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing
CC       premature stop codons. Is recruited by release factors to stalled
CC       ribosomes together with smg1 and smg9 (forming the SMG1C protein kinase
CC       complex) and, in the SMG1C complex, is required to mediate the
CC       recruitment of smg1 to the ribosome:SURF complex and to suppress smg1
CC       kinase activity until the ribosome:SURF complex locates the exon
CC       junction complex (EJC). Acts as a regulator of kinase activity (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SMG1C complex composed of smg1, smg8 and
CC       smg9. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SMG8 family. {ECO:0000305}.
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DR   EMBL; BC121322; AAI21323.1; -; mRNA.
DR   RefSeq; NP_001072291.1; NM_001078823.1.
DR   AlphaFoldDB; Q0VA04; -.
DR   SMR; Q0VA04; -.
DR   STRING; 8364.ENSXETP00000002451; -.
DR   PaxDb; Q0VA04; -.
DR   PRIDE; Q0VA04; -.
DR   Ensembl; ENSXETT00000002451; ENSXETP00000002451; ENSXETG00000001117.
DR   GeneID; 779744; -.
DR   KEGG; xtr:779744; -.
DR   CTD; 55181; -.
DR   Xenbase; XB-GENE-965595; smg8.
DR   eggNOG; KOG3692; Eukaryota.
DR   HOGENOM; CLU_008116_0_0_1; -.
DR   InParanoid; Q0VA04; -.
DR   OMA; HCTVKDS; -.
DR   OrthoDB; 645776at2759; -.
DR   PhylomeDB; Q0VA04; -.
DR   TreeFam; TF323445; -.
DR   Reactome; R-XTR-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000001117; Expressed in ovary and 12 other tissues.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
DR   GO; GO:0045859; P:regulation of protein kinase activity; ISS:UniProtKB.
DR   InterPro; IPR028802; SMG8.
DR   InterPro; IPR019354; SMG8/SMG9.
DR   PANTHER; PTHR13091; PTHR13091; 1.
DR   Pfam; PF10220; Smg8_Smg9; 1.
PE   2: Evidence at transcript level;
KW   Nonsense-mediated mRNA decay; Reference proteome.
FT   CHAIN           1..915
FT                   /note="Nonsense-mediated mRNA decay factor SMG8"
FT                   /id="PRO_0000304976"
FT   REGION          207..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          520..541
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          574..661
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          733..755
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        591..608
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        609..631
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        646..661
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   915 AA;  103027 MW;  BA85B63E19B2AC91 CRC64;
     MVVCGGPTVL RDLLGADTEP TWKDEEVCVV GLFGKTALAQ GSWHKCSLIN SLCDRHIFPL
     FHRAPERPSE RSLFQTYYEQ ESRVLYVLLA GLSDTGSLLK ACEELSRGVS HAEAHEWWKD
     EEKLYCMHLL YLFSVCHILV LVHPTCCFDI TYEKLFRALD SLRQKMLPSL KPSLKDCAVG
     LDWKLNARPC PPRLLFIFQL NGALKVEPKS QGPQTNEKPK KHSPKRRLQH ALEDQIYRIF
     RKSRVLTNQS INCLFTVPAN QAFVYIVADE DEDPVNMLLE GLRHNCTLKD TESLVPISGP
     RRYQMMRHTR QLSFTVENNT SLSGQLVDCT LREFLFQHVE LVLTKKGFDD SVGRNPQPSH
     FELPTYQKWV SVALKLYEII IENKDDDPPA FPGGFPPKLL ANMKVLEGYL DADTKFSENR
     CQKALPMAHS AYQSNLPHNY TTTVHKNQLA QALRVYSQHA RGPAFHKYAM VLNEDCYKFW
     SSGHQLCEER SLTDQHCVHK FHLLPKSGEK IEPERNPPIL FHNSRARSTG SCNCGKKQAP
     REDPFDIKSA NYDFYQILEE KCCGKLDHIT FPIFQPSTPD PAPAKNEASP AAHDGEVEGE
     KMKDKEPQTQ GESTSLSLAL SLGQSTDSLG TFPEGPKAGG DNTEASGHGT DEKVEKRPSL
     VDRQASTVEY LPGMVHLNSP KGLLPKFSSW ALVKLGPAKS YNFHTGLDHL GFIPGTSYLM
     PWDIVIRTKP EDESDLDTNS WPAPNKSIPG KRNSVVMGRG RRRDDIARAF VGFEYEDSRG
     RRFMCSGPDK IMKAIGNGPK ESAIKALNTD MPLYMLSPSQ GRGLKPHYAQ LMRLFVVVPD
     APVQIILAPQ VQPGPPPCPV FVPEKQEITL PSDGLWVLRF PFSYASERGP CYSPKENQQL
     PSYKVMKGIL RTVTQ