BIM2_ARATH
ID BIM2_ARATH Reviewed; 311 AA.
AC Q9CAA4; Q8LC47; Q8RXV7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Transcription factor BIM2;
DE AltName: Full=BES1-interacting Myc-like protein 2;
DE AltName: Full=Basic helix-loop-helix protein 102;
DE Short=AtbHLH102;
DE Short=bHLH 102;
DE AltName: Full=Transcription factor EN 125;
DE AltName: Full=bHLH transcription factor bHLH102;
GN Name=BIM2; Synonyms=BHLH102, EN125; OrderedLocusNames=At1g69010;
GN ORFNames=T6L1.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 52-311, TISSUE SPECIFICITY, INDUCTION, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP IDENTIFICATION, AND INTERACTION WITH BZR2/BES1.
RX PubMed=15680330; DOI=10.1016/j.cell.2004.11.044;
RA Yin Y., Vafeados D., Tao Y., Yoshida S., Asami T., Chory J.;
RT "A new class of transcription factors mediates brassinosteroid-regulated
RT gene expression in Arabidopsis.";
RL Cell 120:249-259(2005).
CC -!- FUNCTION: Positive brassinosteroid-signaling protein.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with the N-terminus of
CC BZR2/BES1. {ECO:0000269|PubMed:15680330, ECO:0000305}.
CC -!- INTERACTION:
CC Q9CAA4; Q84R27: At3g18960; NbExp=4; IntAct=EBI-4476396, EBI-2309076;
CC Q9CAA4; Q9LEZ3: BIM1; NbExp=14; IntAct=EBI-4476396, EBI-617095;
CC Q9CAA4; Q9CAA4: BIM2; NbExp=6; IntAct=EBI-4476396, EBI-4476396;
CC Q9CAA4; Q9FMB6: BIM3; NbExp=6; IntAct=EBI-4476396, EBI-617113;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Repressed by heat treatment. {ECO:0000269|PubMed:12679534}.
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DR EMBL; AC011665; AAG51594.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34875.1; -; Genomic_DNA.
DR EMBL; BT002352; AAN86185.1; -; mRNA.
DR EMBL; AY080650; AAL85017.1; -; mRNA.
DR EMBL; AY086798; AAM63847.1; -; mRNA.
DR EMBL; AF488627; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; E96714; E96714.
DR RefSeq; NP_177064.1; NM_105572.5.
DR AlphaFoldDB; Q9CAA4; -.
DR SMR; Q9CAA4; -.
DR BioGRID; 28454; 9.
DR IntAct; Q9CAA4; 7.
DR STRING; 3702.AT1G69010.1; -.
DR iPTMnet; Q9CAA4; -.
DR PaxDb; Q9CAA4; -.
DR PRIDE; Q9CAA4; -.
DR ProteomicsDB; 240837; -.
DR EnsemblPlants; AT1G69010.1; AT1G69010.1; AT1G69010.
DR GeneID; 843233; -.
DR Gramene; AT1G69010.1; AT1G69010.1; AT1G69010.
DR KEGG; ath:AT1G69010; -.
DR Araport; AT1G69010; -.
DR TAIR; locus:2205455; AT1G69010.
DR eggNOG; ENOG502QUDM; Eukaryota.
DR HOGENOM; CLU_030221_0_0_1; -.
DR InParanoid; Q9CAA4; -.
DR OMA; GSHNMID; -.
DR OrthoDB; 893619at2759; -.
DR PhylomeDB; Q9CAA4; -.
DR PRO; PR:Q9CAA4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAA4; baseline and differential.
DR Genevisible; Q9CAA4; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR044295; BIM1/2/3.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR46412; PTHR46412; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..311
FT /note="Transcription factor BIM2"
FT /id="PRO_0000127154"
FT DOMAIN 45..95
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..311
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 6
FT /note="G -> R (in Ref. 4; AAM63847)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="V -> F (in Ref. 3; AAL85017)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="G -> R (in Ref. 4; AAM63847)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 311 AA; 34487 MW; CC72A040A1A2E427 CRC64;
MRTGKGNQEE EDYGEEDFNS KREGPSSNTT VHSNRDSKEN DKASAIRSKH SVTEQRRRSK
INERFQILRE LIPNSEQKRD TASFLLEVID YVQYLQEKVQ KYEGSYPGWS QEPTKLTPWR
NNHWRVQSLG NHPVAINNGS GPGIPFPGKF EDNTVTSTPA IIAEPQIPIE SDKARAITGI
SIESQPELDD KGLPPLQPIL PMVQGEQANE CPATSDGLGQ SNDLVIEGGT ISISSAYSHE
LLSSLTQALQ NAGIDLSQAK LSVQIDLGKR ANQGLTHEEP SSKNPLSYDT QGRDSSVEEE
SEHSHKRMKT L
