BIME_EMENI
ID BIME_EMENI Reviewed; 2067 AA.
AC P24686; C8VJM8; Q5B9K8;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Negative regulator of mitosis;
DE AltName: Full=Anaphase-promoting complex subunit 1;
GN Name=bimE; ORFNames=AN2772;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1697851; DOI=10.1016/s0021-9258(17)46198-9;
RA Engle D.B., Osmani S.A., Osmani A.H., Rosborough S., Xiang X., Morris N.R.;
RT "A negative regulator of mitosis in Aspergillus is a putative membrane-
RT spanning protein.";
RL J. Biol. Chem. 265:16132-16137(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
CC -!- FUNCTION: Negative regulator of mitosis in E.nidulans. This protein is
CC part of a regulatory pathway that includes the nimA protein kinase. It
CC is required to prevent premature entry into mitosis. Mutations to this
CC protein both cause cells to enter mitosis and prevent them from leaving
CC mitosis.
CC -!- SIMILARITY: Belongs to the APC1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA51478.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CBF84043.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=EAA63206.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M59705; AAA51478.1; ALT_INIT; mRNA.
DR EMBL; AACD01000049; EAA63206.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BN001306; CBF84043.1; ALT_FRAME; Genomic_DNA.
DR PIR; A37879; BWASBE.
DR RefSeq; XP_660376.1; XM_655284.1.
DR AlphaFoldDB; P24686; -.
DR SMR; P24686; -.
DR STRING; 162425.CADANIAP00010334; -.
DR PRIDE; P24686; -.
DR EnsemblFungi; EAA63206; EAA63206; AN2772.2.
DR GeneID; 2874447; -.
DR KEGG; ani:AN2772.2; -.
DR eggNOG; KOG1858; Eukaryota.
DR HOGENOM; CLU_000746_0_0_1; -.
DR InParanoid; P24686; -.
DR OrthoDB; 738153at2759; -.
DR Proteomes; UP000000560; Chromosome VI.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0060090; F:molecular adaptor activity; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR024990; Apc1.
DR InterPro; IPR011989; ARM-like.
DR PANTHER; PTHR12827; PTHR12827; 1.
DR Pfam; PF12859; ANAPC1; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Mitosis; Reference proteome; Repeat.
FT CHAIN 1..2067
FT /note="Negative regulator of mitosis"
FT /id="PRO_0000215873"
FT REPEAT 1434..1465
FT /note="PC 1"
FT REPEAT 1482..1520
FT /note="PC 2"
FT REPEAT 1532..1562
FT /note="PC 3"
FT REPEAT 1625..1659
FT /note="PC 4"
FT REGION 100..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2020..2042
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 336..347
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 100..118
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..370
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 452..479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2021..2035
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1620
FT /note="F -> K (in Ref. 2; EAA63206/CBF84043)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2067 AA; 228577 MW; C7E3CE59C28A60AC CRC64;
MSTVRSLGLH DSSAISFLIA EDLLPAEPSE ELYSWTTSIN NGPNGPVEDE LVWTKSCVVW
TRAGVIKRVF RLDFEKEDIK YALLTNFAVN NVKRTSDLSS LAIPQTTSQQ SNRPSGSESL
DGKESRRSST SKHSSRAVVV VLKTQAHIFF LEGNSHVVPL PFEVDSVFAT PRGLLFQRKV
ADESNTSSYP MVPPNSFMSF SQDFCASQFL DYTSGKVQRP SLSTIPAQSP SWKSRPNKRA
DLPRVFSLMD PHSEMGLVVT NQASRWLHTS LSGRPSGFDV LDPADEIVYV SPRDELAGTF
RADPKSPLIL VLTVNTITGL YTLWTARYRD NESIPSHRKK KRRDTGGTRS KRRSSHFGMA
TGTTTPGARP SAGRESFGPR GDNWNASVMS HSQYSTEGRP DDDEDDFASK LGQDFGEIGV
PSKTSRRVSS MLARADLATS QDRITFSDLV TGSQSSTIHP GGLRQSIGAG STRGSFGFNP
RSSLPPGAGS IYSTTSSFVD PPVDKLLEEL NNESLFEGIE NMDLKESAAG LPEEVFLSKV
ESFSSKFSGS FLAPSKIKSS KRLKVVTLCP TDYASSHVGE STSMALYLVD QEAKSLTVVN
IRVESVKKPA KDVVFLKRSK NKATSDERAL LVQASGIQHV SGTLDVCKVV DGGLSRIITL
SVNDAGATVL HLQMPWNDPI IIELPSKFML HEMDALSSIM TVNTSREGSV NRVMADFSMT
MTGLDHPAVD GKFDVVDSAK RRHKLQLCME PTNPLVRRAF GVCRFALCGY FPDKVADGLL
MGWWKTLKWL QEREVCENDL EWTALVVTLF AQAIPFIEGD QSGGTTRLTR RKRGLLRSSS
GSYVDTESWE SMLEQESGSA GVVASWMNTA SWGWVVEQDA EDESTASYGR KPNKEPLSSS
RSTCRKNTYL LRCATLTREF LRTPQGIAAV GCDGHLRKAL SNSEHSIHTP LCTILVALHL
LREEQKLSAC DEEQSHKTLG LLAPVLAQLG GWLGWPSWNW AEDSYYGSEI ASINRWQFEN
TRMAGPDLPA EPLPPPSIFA YLEKAWRGES CQFWTLLNLV NFDGHPRRGR LWQWCSTLTP
RTLALEGFVS EMRHKLSVLE RIQLLHRWGL TRSVIETFPA GISTPLYEAI IESQTHASTS
WSSSFLGLID RDDLNISSQS STTRPPPPLS LNVSHDAIRD YHQISNSTLD IDAINSFEAS
AEADRFSVTR LIFREDKRFI EAARLLNQSK APAAECHPEP EWTDSDLLEA QKEVVQLVTL
RTLSIPTGRA MLAFSGRLPL LTEKLPIPSF SLQCVMKPSN VTISADRASF HEEKICWAFF
HNGVSTGLAI SKNSKGIDTS WILFNKPQEL TNRHAGFLLA LGLNGHLRSL AKWVAFKYLT
PKHTMTSIGL LLGLSASYLG TMDTLVTRLL SVHVTRMLPM GAAELNLSPL TQTAGIMGIG
LLYCNSQHRR MSEVMLSEIE NADQEEGSAT NDYLRNEGYR LAAGFALGFI NLGKGKDLKG
MRDMHIVERL LAVAVGTKNV DLAHVLDRAT AGATIALAII FMKTNDETLA QKVDIPDTTV
RFDYVRPDLF LLRTLARHII MWDRIQACDE WFIGSLPEVY RRRYRLTGVR RLKSNDMPFF
NIIAGLCFAL GLRFAGSPDP TVRDILLSYL DQFIRISRLP APNYDARLAR NSVRHCQDVV
ALSLAAVMAG TGDLALFRRL RSLHGRVDPD TPYGSHMAAH MAIGMLFLGG GSYTLGTSNL
AVASLICSLY PIFPTTVLDN ECHLQAFRHL WVLAAEPRCI VPRDLDSRRP ISMPITVTDS
DGVSGTLTAP CLLPDLNRIA KVEVLSPDYW PLVLDFDSNP GVREKFQQGD QSIYLRRKAT
YNPTGSSFFS STLAGLSSAQ DILPSASTSA SNQGKGLSPS ALPNVSALLS KESHRFTPKP
AAQSIWDWIF QLDSLHGILD AREKELVLPS SFPARPRPSS TPHDAPWLRQ SAVDSKLLAE
SIAQNVIQSA TGRGGNPDEV RDRLWQLRLL FAWIDAGSSF PSESDEEKRD RQETGSMPSS
GHWLRRDYVE DLRWKIWGVQ ARGSEGS
