SMIM4_MOUSE
ID SMIM4_MOUSE Reviewed; 80 AA.
AC Q8C1Q6; Q8BZ92; Q8C1P1;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Small integral membrane protein 4 {ECO:0000305};
DE AltName: Full=Small nucleolar RNA host gene 8;
GN Name=Smim4 {ECO:0000312|MGI:MGI:1913737};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Pancreas, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mitochondrial ribosome-associated protein involved in the
CC first steps of cytochrome c oxidase complex (complex IV) assembly.
CC {ECO:0000250|UniProtKB:Q8WVI0}.
CC -!- SUBUNIT: Associates with the mitochondrial ribosome. Interacts with
CC BRAWNIN. Interacts with MT-CYB; interacts with newly synthesizes MT-
CC CYB. {ECO:0000250|UniProtKB:Q8WVI0}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8WVI0}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8C1Q6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C1Q6-2; Sequence=VSP_035265;
CC Name=3;
CC IsoId=Q8C1Q6-3; Sequence=VSP_035266, VSP_035267;
CC -!- SIMILARITY: Belongs to the SMIM4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25173.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC25215.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK007443; BAC25173.1; ALT_INIT; mRNA.
DR EMBL; AK008341; BAC25215.1; ALT_INIT; mRNA.
DR EMBL; AK036279; BAC29371.1; -; mRNA.
DR CCDS; CCDS84104.1; -. [Q8C1Q6-2]
DR CCDS; CCDS84105.1; -. [Q8C1Q6-1]
DR RefSeq; NP_001295020.1; NM_001308091.1. [Q8C1Q6-1]
DR RefSeq; NP_001295393.1; NM_001308464.1. [Q8C1Q6-2]
DR RefSeq; NP_001295395.1; NM_001308466.1.
DR AlphaFoldDB; Q8C1Q6; -.
DR STRING; 10090.ENSMUSP00000067418; -.
DR iPTMnet; Q8C1Q6; -.
DR PhosphoSitePlus; Q8C1Q6; -.
DR EPD; Q8C1Q6; -.
DR MaxQB; Q8C1Q6; -.
DR PaxDb; Q8C1Q6; -.
DR PeptideAtlas; Q8C1Q6; -.
DR PRIDE; Q8C1Q6; -.
DR ProteomicsDB; 261435; -. [Q8C1Q6-1]
DR Antibodypedia; 64270; 40 antibodies from 7 providers.
DR Ensembl; ENSMUST00000049732; ENSMUSP00000050634; ENSMUSG00000058351. [Q8C1Q6-1]
DR Ensembl; ENSMUST00000090205; ENSMUSP00000087673; ENSMUSG00000058351. [Q8C1Q6-3]
DR Ensembl; ENSMUST00000203261; ENSMUSP00000145018; ENSMUSG00000058351. [Q8C1Q6-2]
DR GeneID; 66487; -.
DR KEGG; mmu:66487; -.
DR UCSC; uc007swr.2; mouse. [Q8C1Q6-1]
DR CTD; 440957; -.
DR MGI; MGI:1913737; Smim4.
DR VEuPathDB; HostDB:ENSMUSG00000058351; -.
DR GeneTree; ENSGT00390000013390; -.
DR HOGENOM; CLU_2276535_0_0_1; -.
DR InParanoid; Q8C1Q6; -.
DR OMA; GMYRFLP; -.
DR OrthoDB; 1589691at2759; -.
DR PhylomeDB; Q8C1Q6; -.
DR TreeFam; TF324402; -.
DR BioGRID-ORCS; 66487; 0 hits in 2 CRISPR screens.
DR ChiTaRS; Smim4; mouse.
DR PRO; PR:Q8C1Q6; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8C1Q6; protein.
DR Bgee; ENSMUSG00000058351; Expressed in facial nucleus and 223 other tissues.
DR ExpressionAtlas; Q8C1Q6; baseline and differential.
DR Genevisible; Q8C1Q6; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR InterPro; IPR028183; UPF0640.
DR PANTHER; PTHR35250; PTHR35250; 1.
DR Pfam; PF15114; UPF0640; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..80
FT /note="Small integral membrane protein 4"
FT /id="PRO_0000349244"
FT TOPO_DOM 1..19
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:Q8WVI0"
FT TRANSMEM 20..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..80
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:Q8WVI0"
FT VAR_SEQ 51..80
FT /note="YDVYRRKASERQYQRRLEDTSETNLHKLIK -> CFLPEMFKMKTTKHLSLD
FT LANIPSKQRITDLCARSSTKVLSRCDVVLSYLST (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035265"
FT VAR_SEQ 51..60
FT /note="YDVYRRKASE -> FSGLVRWLSS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035266"
FT VAR_SEQ 61..80
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035267"
SQ SEQUENCE 80 AA; 9746 MW; 66D1F74058F82515 CRC64;
MFSRAQVRRA LQRVPGKQRF GIYRFLPFFF VLGGAMEWIM IKVRVGQETF YDVYRRKASE
RQYQRRLEDT SETNLHKLIK
