SMK3A_MOUSE
ID SMK3A_MOUSE Reviewed; 504 AA.
AC C0HKC8; Q9QYZ5;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 1.
DT 03-AUG-2022, entry version 28.
DE RecName: Full=Sperm motility kinase 3A {ECO:0000312|MGI:MGI:3693943};
DE EC=2.7.11.1 {ECO:0000250|UniProtKB:Q9QYZ3};
GN Name=Smok3a {ECO:0000312|MGI:MGI:3693943};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis {ECO:0000312|EMBL:CAB61342.1};
RX PubMed=10647005; DOI=10.1038/45970;
RA Herrmann B.G., Koschorz B., Wertz K., McLaughlin K.J., Kispert A.;
RT "A protein kinase encoded by the t complex responder gene causes non-
RT Mendelian inheritance.";
RL Nature 402:141-146(1999).
CC -!- FUNCTION: May play a role in sperm motility, especially in the
CC regulation of flagellar function. {ECO:0000269|PubMed:10647005}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000250|UniProtKB:Q9QYZ3};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000250|UniProtKB:Q9QYZ3};
CC -!- TISSUE SPECIFICITY: Testis-specific. Expressed in the testis from 22
CC days postpartum (22 dpp). {ECO:0000269|PubMed:10647005}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr
CC protein kinase family. Smok subfamily. {ECO:0000305}.
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DR EMBL; AJ245454; CAB61342.1; -; mRNA.
DR CCDS; CCDS51678.1; -.
DR RefSeq; NP_001034978.1; NM_001039889.3.
DR RefSeq; NP_001119517.1; NM_001126045.1.
DR RefSeq; NP_001162073.1; NM_001168602.1.
DR AlphaFoldDB; C0HKC8; -.
DR SMR; C0HKC8; -.
DR STRING; 10090.ENSMUSP00000083049; -.
DR iPTMnet; C0HKC8; -.
DR PhosphoSitePlus; C0HKC8; -.
DR DNASU; 545814; -.
DR Ensembl; ENSMUST00000085886; ENSMUSP00000083049; ENSMUSG00000075599.
DR Ensembl; ENSMUST00000100537; ENSMUSP00000098105; ENSMUSG00000079156.
DR Ensembl; ENSMUST00000166206; ENSMUSP00000129450; ENSMUSG00000079156.
DR Ensembl; ENSMUST00000171498; ENSMUSP00000125938; ENSMUSG00000075599.
DR GeneID; 545814; -.
DR GeneID; 622474; -.
DR KEGG; mmu:545814; -.
DR KEGG; mmu:622474; -.
DR CTD; 545814; -.
DR CTD; 622474; -.
DR MGI; MGI:3693943; Smok3a.
DR VEuPathDB; HostDB:ENSMUSG00000075599; -.
DR VEuPathDB; HostDB:ENSMUSG00000079156; -.
DR eggNOG; KOG0586; Eukaryota.
DR OMA; PCKELIP; -.
DR OrthoDB; 1127668at2759; -.
DR BioGRID-ORCS; 545814; 1 hit in 33 CRISPR screens.
DR BioGRID-ORCS; 622474; 2 hits in 32 CRISPR screens.
DR PRO; PR:C0HKC8; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; C0HKC8; protein.
DR Bgee; ENSMUSG00000075599; Expressed in testis and 2 other tissues.
DR ExpressionAtlas; C0HKC8; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0050321; F:tau-protein kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..504
FT /note="Sperm motility kinase 3A"
FT /id="PRO_0000307871"
FT DOMAIN 28..276
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 294..334
FT /note="UBA"
FT /evidence="ECO:0000255"
FT REGION 389..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 441..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..406
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 147
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 34..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 57
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 504 AA; 56487 MW; BD5FAEB0C61270C4 CRC64;
MGPGSQQKSE KLRSKSPLAD MDGLHAQYVM LETIGHGGCA TVKLAQHRLT GTHVAVKTIR
KREYWCNRVI SEVELLMMAD HPNIISLLQV IETKKKVYLI MELCKGKSLY QHIRKAGYLQ
EHEARALFKQ LLSAMNYCHN QGIVHRDLKP DNIMVEKDGK VKIIDFGLGT KVKPGQKLNL
FCGTYPFSAP EVLLSTPYDG PKIDVWTLGV VLYFMVTGKI PFDACSIKKL VKRILAGKYS
IPSRLSAELQ DLLSLLMTAN PKLRPTVAEV MVHPWVTEGS GVFPDPCEEQ TPLKPDPAIV
KAMGHIGFQA QDIEDSLRQR KFNQTMASYC LLKKQILKEC DRPTRARPVN PSVTPFPSLV
DTATTRLGLR RRENEPTCPW SSANRQVSVC GKSTSKKRDR RVSWPSVLGR PRHTAPTMDH
TRTRTRSVPC ICSMFCTVQP NSSEESTQGH TRASAADKPV HSRGWPRGIK GWTRMIGNAM
RKLCCCIPSK ETSHLGQNRV SPKK
