SMOK1_MOUSE
ID SMOK1_MOUSE Reviewed; 484 AA.
AC Q9QYZ4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Sperm motility kinase 1;
DE EC=2.7.11.1;
GN Name=Smok1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND FUNCTION.
RC TISSUE=Testis;
RX PubMed=10647005; DOI=10.1038/45970;
RA Herrmann B.G., Koschorz B., Wertz K., McLaughlin K.J., Kispert A.;
RT "A protein kinase encoded by the t complex responder gene causes non-
RT Mendelian inheritance.";
RL Nature 402:141-146(1999).
CC -!- FUNCTION: May play a role in sperm motility, especially in the
CC regulation of flagellar function. {ECO:0000269|PubMed:10647005}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- TISSUE SPECIFICITY: Testis-specific. Expressed in the testis from 22
CC days postpartum (22 dpp). {ECO:0000269|PubMed:10647005}.
CC -!- MISCELLANEOUS: Tw12 allele.
CC -!- MISCELLANEOUS: Encoded on the T-complex, a region of 20-30 Mb on
CC proximal third of mouse chromosome 17. Naturally occurring variant
CC forms of the T-complex, known as complete t-haplotypes, are found in
CC wild mouse populations. The t-haplotypes contain at least four
CC nonoverlapping inversions that suppress recombination with the wild-
CC type chromosome, and lock into strong linkage disequilibrium loci
CC affecting normal transmission of the chromosome, male gametogenesis and
CC embryonic development.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein
CC kinase family. Smok subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ245455; CAB61343.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9QYZ4; -.
DR SMR; Q9QYZ4; -.
DR PRIDE; Q9QYZ4; -.
DR MGI; MGI:1351488; Smok1.
DR InParanoid; Q9QYZ4; -.
DR PRO; PR:Q9QYZ4; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9QYZ4; protein.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IDA:MGI.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IDA:MGI.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.
FT CHAIN 1..484
FT /note="Sperm motility kinase 1"
FT /id="PRO_0000307870"
FT DOMAIN 8..256
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 274..314
FT /note="UBA"
FT REGION 423..447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 430..444
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 127
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10028"
FT BINDING 14..22
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 484 AA; 54794 MW; 4244F91CF74D50AA CRC64;
MEKFHAQYEM LETIGQGGCA KVKLARHRLT GTHVAVKMIP KREYWCKLLM FEAELLMMFN
HPNIISLLQV IETKKKVYLI MELCEGKSLY QHIQNAGYLQ EDEARPLFKQ LLSAMNYCHN
QGIVHRDLTP DNIMVEKDGR VKNIDFGLST HVKPGQKLNL FCGTYPFSAP EVLLSRPYGG
PKIDVWTLGV VLYFMVIGKI PFDAASIEKL RKQIVAGKYS APCRLSVKLQ HLINLLMTDN
PELRPTVAEV MVHPWITKGS GVFPDPCEEQ IPLKPDPAIV KPMGHIGFQA QDIEDSLRQR
KFNETMASYC LLKKQILKEC DRPIRDQPMN PSVTPFPSLV DTPTFHLGLR RRETEPTGLR
LSANRQVSVC GKSTSKKRDR SFIWPGVLSR PINTTPTMDQ THTRTRSVPC IYSNVCTIHP
NSIDESTEGH TSASAEDKPV HSRGWPRGIK GWTRKIGNAM RKLCCCIPSK ETSHLGQSRV
CPKK