SMP1_YEAST
ID SMP1_YEAST Reviewed; 452 AA.
AC P38128; D6VQH6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Transcription factor SMP1;
GN Name=SMP1; OrderedLocusNames=YBR182C; ORFNames=YBR1245;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-242.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7871891; DOI=10.1002/yea.320101116;
RA Demolis N., Jacquet M., Mallet L.;
RT "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes
RT encoding ribosomal proteins and six putative new genes, one of which
RT encodes a putative transcriptional factor.";
RL Yeast 10:1511-1525(1994).
RN [5]
RP CHARACTERIZATION.
RX PubMed=9121433; DOI=10.1128/mcb.17.4.1848;
RA Dodou E., Treisman R.;
RT "The Saccharomyces cerevisiae MADS-box transcription factor Rlm1 is a
RT target for the Mpk1 mitogen-activated protein kinase pathway.";
RL Mol. Cell. Biol. 17:1848-1859(1997).
RN [6]
RP FUNCTION, PHOSPHORYLATION, AND INTERACTION WITH HOG1.
RX PubMed=12482976; DOI=10.1128/mcb.23.1.229-237.2003;
RA de Nadal E., Casadome L., Posas F.;
RT "Targeting the MEF2-like transcription factor Smp1 by the stress-activated
RT Hog1 mitogen-activated protein kinase.";
RL Mol. Cell. Biol. 23:229-237(2003).
CC -!- FUNCTION: Transcription factor that controls part of the HOG1-mediated
CC osmostress responses. Binds to the DNA sequence 5'-ACTACTA[TA](4)TAG-
CC 3'. Does not appear to function in the MPK1 pathway.
CC {ECO:0000269|PubMed:12482976}.
CC -!- SUBUNIT: Can heterodimerize with RLM1. Interacts with HOG1.
CC {ECO:0000269|PubMed:12482976}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- PTM: Phosphorylated by HOG1. {ECO:0000269|PubMed:12482976}.
CC -!- SIMILARITY: Belongs to the MEF2 family. {ECO:0000305}.
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DR EMBL; Z36051; CAA85143.1; -; Genomic_DNA.
DR EMBL; AY693072; AAT93091.1; -; Genomic_DNA.
DR EMBL; U02073; AAB60287.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07296.1; -; Genomic_DNA.
DR PIR; S46054; S46054.
DR RefSeq; NP_009741.3; NM_001178530.3.
DR AlphaFoldDB; P38128; -.
DR SMR; P38128; -.
DR BioGRID; 32880; 115.
DR IntAct; P38128; 4.
DR MINT; P38128; -.
DR STRING; 4932.YBR182C; -.
DR iPTMnet; P38128; -.
DR PaxDb; P38128; -.
DR PRIDE; P38128; -.
DR EnsemblFungi; YBR182C_mRNA; YBR182C; YBR182C.
DR GeneID; 852480; -.
DR KEGG; sce:YBR182C; -.
DR SGD; S000000386; SMP1.
DR VEuPathDB; FungiDB:YBR182C; -.
DR eggNOG; KOG0014; Eukaryota.
DR GeneTree; ENSGT00940000169350; -.
DR HOGENOM; CLU_605820_0_0_1; -.
DR InParanoid; P38128; -.
DR OMA; ATNDACQ; -.
DR BioCyc; YEAST:G3O-29126-MON; -.
DR Reactome; R-SCE-525793; Myogenesis.
DR PRO; PR:P38128; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38128; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0008301; F:DNA binding, bending; IDA:SGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IC:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..452
FT /note="Transcription factor SMP1"
FT /id="PRO_0000199441"
FT DOMAIN 3..57
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DNA_BIND 58..87
FT /note="Mef2-type"
FT /evidence="ECO:0000255"
FT REGION 97..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 452 AA; 49414 MW; 3D53A859D468227C CRC64;
MGRRKIEIEP IKDDRNRTVT FIKRKAGLFK KAHELSVLCQ VDIAVIILGS NNTFYEYSSV
DMSNLLNVHQ NNTDLPHNII EPSDYGDYVK KPRVVLNERK RRRRRATVLQ PASHSGSCTV
SSQDSSSVQN NGNLSAPLAS NDAGNAGVST PLVHCHGAIS RSGSNHSDCA RNSADYQMLQ
GGLNSGGSFH ANDYKESVDQ QHVANEAIHR NFMNKRIRPD THLLLSESNH SNYHNFYPSP
YENLPKPSLP ASLVGNIPSF QSQFVQVIPA NSNPMGKGFN GTGDSESFEA KQKIHPTVAI
SNTLEGPAPV QAMVHHLHQL NSNRGKLSGK PYLKLNIPKA TNDACQRSPA MYSGTASPKT
DVQATPNQML ASNMSSPLSR SKFLGFKNND MDDLYHNGRC GSTYVNNKTF FLKPPIGRPP
KFPKSPSSSI VVFPSSVASS TLKSTSSTNS PD
