SMP3_CANAL
ID SMP3_CANAL Reviewed; 498 AA.
AC Q5A0L9; A0A1D8PGU9;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=GPI mannosyltransferase 4;
DE EC=2.4.1.-;
DE AltName: Full=CaSMP3;
DE AltName: Full=GPI mannosyltransferase IV;
DE Short=GPI-MT-IV;
GN Name=SMP3; OrderedLocusNames=CAALFM_C203070CA;
GN ORFNames=CaO19.13214, CaO19.5792;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP IDENTIFICATION.
RX PubMed=15470093; DOI=10.1099/mic.0.27254-0;
RA Grimme S.J., Colussi P.A., Taron C.H., Orlean P.;
RT "Deficiencies in the essential Smp3 mannosyltransferase block
RT glycosylphosphatidylinositol assembly and lead to defects in growth and
RT cell wall biogenesis in Candida albicans.";
RL Microbiology 150:3115-3128(2004).
CC -!- FUNCTION: Alpha-1,2-mannosyltransferase involved in
CC glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth
CC mannose to trimannosyl-GPIs during GPI precursor assembly. The presence
CC of a fourth mannose in GPI is essential in fungi (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- MISCELLANEOUS: May be used as a target for the development of some new
CC fungicide, due the fact the presence of a fourth mannose in GPI-anchor
CC proteins is essential for viability in fungi but not in mammals.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGZ
CC subfamily. {ECO:0000305}.
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DR EMBL; CP017624; AOW27344.1; -; Genomic_DNA.
DR RefSeq; XP_715333.2; XM_710240.2.
DR AlphaFoldDB; Q5A0L9; -.
DR STRING; 237561.Q5A0L9; -.
DR GeneID; 3642980; -.
DR KEGG; cal:CAALFM_C203070CA; -.
DR CGD; CAL0000175734; SMP3.
DR VEuPathDB; FungiDB:C2_03070C_A; -.
DR HOGENOM; CLU_022957_2_0_1; -.
DR InParanoid; Q5A0L9; -.
DR OrthoDB; 821144at2759; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:Q5A0L9; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0000030; F:mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR GO; GO:0006276; P:plasmid maintenance; IEA:EnsemblFungi.
DR InterPro; IPR005599; GPI_mannosylTrfase.
DR PANTHER; PTHR22760; PTHR22760; 1.
DR Pfam; PF03901; Glyco_transf_22; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..498
FT /note="GPI mannosyltransferase 4"
FT /id="PRO_0000246274"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 408
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 473
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 498 AA; 57971 MW; 98A3C4952410BCE5 CRC64;
MIKINFNWRT FYLLTIVFRF VFTLSDSYIH PDEHFQSLEV LTNRILNYST NIPWEFQDDP
ARSLAPLYFI YGPLLYFIKF FKLNLTALQI WYIARLQISI LSWIITDFCL YWMLPSKPER
IKAIFFTSTS YITLVYQNHL FSNSIETLLL LVTILLIDDL RYVQESKDQD VQNLNKNKNL
FYTGVLISLG IFNRITFPAF LILPGWFVMK YVLKHYVSGL YLVMGFFSTT ALLILVDTIL
FGNINNVVAE PFNVSSYIIA PLNNLLYNAR YENLAQHGIH PYYTHILVNM PQILGPGLIF
FVSKSYTKTT PFLTVISGLL FLSVIPHQEL RFLIPLLPLA CCSFDFTLKW VQPWMLYTWY
IFNIFMSILM GKLHQGGVVP VLDHIKSEAS VQVWWRTYTP PSWILGSNST ETTHLGEKLN
DNKFINIVDC MGADSKEVQQ ILQTISTNKP VYLITPIASF KHFDESRFSP VWNYTFHLDL
DHLDFADIQP GLGVYQLL
