SMR3_ARATH
ID SMR3_ARATH Reviewed; 115 AA.
AC Q9LZ60; Q8LD37;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Cyclin-dependent protein kinase inhibitor SMR3 {ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
DE AltName: Full=Protein SIAMESE-RELATED 3 {ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
GN Name=SMR3 {ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
GN OrderedLocusNames=At5g02420; ORFNames=T22P11.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17098811; DOI=10.1105/tpc.106.044834;
RA Churchman M.L., Brown M.L., Kato N., Kirik V., Huelskamp M., Inze D.,
RA De Veylder L., Walker J.D., Zheng Z., Oppenheimer D.G., Gwin T.,
RA Churchman J., Larkin J.C.;
RT "SIAMESE, a plant-specific cell cycle regulator, controls endoreplication
RT onset in Arabidopsis thaliana.";
RL Plant Cell 18:3145-3157(2006).
RN [5]
RP INTERACTION WITH CDKA-1 AND D-TYPE CYCLINS.
RX PubMed=20706207; DOI=10.1038/msb.2010.53;
RA Van Leene J., Hollunder J., Eeckhout D., Persiau G., Van De Slijke E.,
RA Stals H., Van Isterdael G., Verkest A., Neirynck S., Buffel Y., De Bodt S.,
RA Maere S., Laukens K., Pharazyn A., Ferreira P.C.G., Eloy N., Renne C.,
RA Meyer C., Faure J.-D., Steinbrenner J., Beynon J., Larkin J.C.,
RA Van de Peer Y., Hilson P., Kuiper M., De Veylder L., Van Onckelen H.,
RA Inze D., Witters E., De Jaeger G.;
RT "Targeted interactomics reveals a complex core cell cycle machinery in
RT Arabidopsis thaliana.";
RL Mol. Syst. Biol. 6:397-397(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24399300; DOI=10.1105/tpc.113.118943;
RA Yi D., Alvim Kamei C.L., Cools T., Vanderauwera S., Takahashi N.,
RA Okushima Y., Eekhout T., Yoshiyama K.O., Larkin J., Van den Daele H.,
RA Conklin P., Britt A., Umeda M., De Veylder L.;
RT "The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5
RT and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen
RT species.";
RL Plant Cell 26:296-309(2014).
RN [7]
RP FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=26546445; DOI=10.1105/tpc.15.00489;
RA Kumar N., Harashima H., Kalve S., Bramsiepe J., Wang K., Sizani B.L.,
RA Bertrand L.L., Johnson M.C., Faulk C., Dale R., Simmons L.A.,
RA Churchman M.L., Sugimoto K., Kato N., Dasanayake M., Beemster G.,
RA Schnittger A., Larkin J.C.;
RT "Functional conservation in the SIAMESE-RELATED family of cyclin-dependent
RT kinase inhibitors in land plants.";
RL Plant Cell 27:3065-3080(2015).
CC -!- FUNCTION: Probable cyclin-dependent protein kinase (CDK) inhibitor that
CC functions as a repressor of mitosis in the endoreduplication cell
CC cycle. {ECO:0000269|PubMed:26546445}.
CC -!- SUBUNIT: Interacts with CDKA-1 and D-type cyclins (PubMed:20706207).
CC {ECO:0000269|PubMed:20706207}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17098811}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots and stems.
CC {ECO:0000269|PubMed:17098811}.
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DR EMBL; AL162971; CAB85979.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90470.1; -; Genomic_DNA.
DR EMBL; AY086229; AAM64305.1; -; mRNA.
DR PIR; T48263; T48263.
DR RefSeq; NP_195862.1; NM_120320.3.
DR AlphaFoldDB; Q9LZ60; -.
DR SMR; Q9LZ60; -.
DR BioGRID; 17112; 4.
DR IntAct; Q9LZ60; 4.
DR STRING; 3702.AT5G02420.1; -.
DR PaxDb; Q9LZ60; -.
DR PRIDE; Q9LZ60; -.
DR ProteomicsDB; 234573; -.
DR EnsemblPlants; AT5G02420.1; AT5G02420.1; AT5G02420.
DR GeneID; 831836; -.
DR Gramene; AT5G02420.1; AT5G02420.1; AT5G02420.
DR KEGG; ath:AT5G02420; -.
DR Araport; AT5G02420; -.
DR TAIR; locus:2181728; AT5G02420.
DR eggNOG; ENOG502S6WR; Eukaryota.
DR HOGENOM; CLU_2174555_0_0_1; -.
DR InParanoid; Q9LZ60; -.
DR OMA; ATILKCP; -.
DR OrthoDB; 1472198at2759; -.
DR PhylomeDB; Q9LZ60; -.
DR PRO; PR:Q9LZ60; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZ60; baseline and differential.
DR Genevisible; Q9LZ60; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0045839; P:negative regulation of mitotic nuclear division; IBA:GO_Central.
DR GO; GO:0032875; P:regulation of DNA endoreduplication; IEA:InterPro.
DR InterPro; IPR040389; SMR.
DR PANTHER; PTHR33142; PTHR33142; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Nucleus; Protein kinase inhibitor; Reference proteome.
FT CHAIN 1..115
FT /note="Cyclin-dependent protein kinase inhibitor SMR3"
FT /id="PRO_0000418066"
FT REGION 17..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 30
FT /note="D -> V (in Ref. 3; AAM64305)"
FT /evidence="ECO:0000305"
FT CONFLICT 87
FT /note="V -> L (in Ref. 3; AAM64305)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 115 AA; 13308 MW; D2605DF0B75EAE86 CRC64;
MAEICCVKEI QEEDVEKIRL PTRPELDIPD SDHEDPTVNE EEGCKTPTSS DHKIPEVKYT
LCPPAPRKPK PNRSSGTKRK LTPVNVVNRI PIDLSREIEM FFEDLDRRIK KSRKQ
