ID   SMR4_ARATH              Reviewed;          72 AA.
AC   Q1JPP8; Q8LEM3; Q9LZL0;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Cyclin-dependent protein kinase inhibitor SMR4 {ECO:0000303|PubMed:17599908, ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
DE   AltName: Full=Protein SIAMESE-RELATED 4 {ECO:0000303|PubMed:17599908, ECO:0000303|PubMed:24399300, ECO:0000303|PubMed:26546445};
GN   Name=SMR4 {ECO:0000303|PubMed:17599908, ECO:0000303|PubMed:24399300,
GN   ECO:0000303|PubMed:26546445};
GN   OrderedLocusNames=At5g02220 {ECO:0000312|Araport:AT5G02220};
GN   ORFNames=T7H20.270 {ECO:0000312|EMBL:CAB82995.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|EMBL:ABF47121.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INDUCTION BY DOUBLE-STRANDED DNA BREAKS-INDUCING TREATMENTS.
RX   PubMed=17227549; DOI=10.1111/j.1365-313x.2006.02931.x;
RA   Culligan K.M., Robertson C.E., Foreman J., Doerner P., Britt A.B.;
RT   "ATR and ATM play both distinct and additive roles in response to ionizing
RT   radiation.";
RL   Plant J. 48:947-961(2006).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17599908; DOI=10.1074/jbc.m703326200;
RA   Peres A., Churchman M.L., Hariharan S., Himanen K., Verkest A.,
RA   Vandepoele K., Magyar Z., Hatzfeld Y., Van Der Schueren E., Beemster G.T.,
RA   Frankard V., Larkin J.C., Inze D., De Veylder L.;
RT   "Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic
RT   and abiotic stresses.";
RL   J. Biol. Chem. 282:25588-25596(2007).
RN   [7]
RP   INTERACTION WITH CDKA-1 AND D-TYPE CYCLINS.
RX   PubMed=20706207; DOI=10.1038/msb.2010.53;
RA   Van Leene J., Hollunder J., Eeckhout D., Persiau G., Van De Slijke E.,
RA   Stals H., Van Isterdael G., Verkest A., Neirynck S., Buffel Y., De Bodt S.,
RA   Maere S., Laukens K., Pharazyn A., Ferreira P.C.G., Eloy N., Renne C.,
RA   Meyer C., Faure J.-D., Steinbrenner J., Beynon J., Larkin J.C.,
RA   Van de Peer Y., Hilson P., Kuiper M., De Veylder L., Van Onckelen H.,
RA   Inze D., Witters E., De Jaeger G.;
RT   "Targeted interactomics reveals a complex core cell cycle machinery in
RT   Arabidopsis thaliana.";
RL   Mol. Syst. Biol. 6:397-397(2010).
RN   [8]
RP   FUNCTION, TISSUE SPECIFICITY, INDUCTION BY DNA DAMAGE, GENE FAMILY, AND
RP   NOMENCLATURE.
RX   PubMed=24399300; DOI=10.1105/tpc.113.118943;
RA   Yi D., Alvim Kamei C.L., Cools T., Vanderauwera S., Takahashi N.,
RA   Okushima Y., Eekhout T., Yoshiyama K.O., Larkin J., Van den Daele H.,
RA   Conklin P., Britt A., Umeda M., De Veylder L.;
RT   "The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5
RT   and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen
RT   species.";
RL   Plant Cell 26:296-309(2014).
RN   [9]
RP   FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=26546445; DOI=10.1105/tpc.15.00489;
RA   Kumar N., Harashima H., Kalve S., Bramsiepe J., Wang K., Sizani B.L.,
RA   Bertrand L.L., Johnson M.C., Faulk C., Dale R., Simmons L.A.,
RA   Churchman M.L., Sugimoto K., Kato N., Dasanayake M., Beemster G.,
RA   Schnittger A., Larkin J.C.;
RT   "Functional conservation in the SIAMESE-RELATED family of cyclin-dependent
RT   kinase inhibitors in land plants.";
RL   Plant Cell 27:3065-3080(2015).
CC   -!- FUNCTION: Probable cyclin-dependent protein kinase (CDK) inhibitor that
CC       functions as a repressor of mitosis in the endoreduplication cell cycle
CC       (PubMed:26546445). Acts as a potent cell cycle inhibitor
CC       (PubMed:24399300). {ECO:0000269|PubMed:24399300,
CC       ECO:0000269|PubMed:26546445}.
CC   -!- SUBUNIT: Interacts with CDKA-1 and D-type cyclins (PubMed:20706207).
CC       {ECO:0000269|PubMed:20706207}.
CC   -!- TISSUE SPECIFICITY: Expressed in root meristems after induction.
CC       {ECO:0000269|PubMed:24399300}.
CC   -!- INDUCTION: Up-regulated by double-stranded DNA breaks-inducing
CC       treatments (PubMed:17227549). Up-regulated by DNA damage
CC       (PubMed:24399300). {ECO:0000269|PubMed:17227549,
CC       ECO:0000269|PubMed:24399300}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82995.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL162508; CAB82995.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90446.1; -; Genomic_DNA.
DR   EMBL; BT025305; ABF47121.1; -; mRNA.
DR   EMBL; AY085350; AAM62581.1; -; mRNA.
DR   PIR; T48243; T48243.
DR   RefSeq; NP_568097.1; NM_120300.2.
DR   AlphaFoldDB; Q1JPP8; -.
DR   IntAct; Q1JPP8; 8.
DR   STRING; 3702.AT5G02220.1; -.
DR   PaxDb; Q1JPP8; -.
DR   PRIDE; Q1JPP8; -.
DR   EnsemblPlants; AT5G02220.1; AT5G02220.1; AT5G02220.
DR   GeneID; 831792; -.
DR   Gramene; AT5G02220.1; AT5G02220.1; AT5G02220.
DR   KEGG; ath:AT5G02220; -.
DR   Araport; AT5G02220; -.
DR   TAIR; locus:2185213; AT5G02220.
DR   eggNOG; ENOG502S9X0; Eukaryota.
DR   HOGENOM; CLU_189464_0_0_1; -.
DR   InParanoid; Q1JPP8; -.
DR   OMA; GCKTPRR; -.
DR   OrthoDB; 1593759at2759; -.
DR   PhylomeDB; Q1JPP8; -.
DR   PRO; PR:Q1JPP8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q1JPP8; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045839; P:negative regulation of mitotic nuclear division; IBA:GO_Central.
DR   GO; GO:0032875; P:regulation of DNA endoreduplication; IEA:InterPro.
DR   InterPro; IPR040389; SMR.
DR   PANTHER; PTHR33142; PTHR33142; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Protein kinase inhibitor; Reference proteome.
FT   CHAIN           1..72
FT                   /note="Cyclin-dependent protein kinase inhibitor SMR4"
FT                   /id="PRO_0000438463"
FT   CONFLICT        71
FT                   /note="C -> S (in Ref. 4; AAM62581)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   72 AA;  8324 MW;  6B27592CFDB37542 CRC64;
     MEVVERKTEM AEEGCTTPRS TMYRIPVASV CPPPPRKKLM VVRKRDPPRN GYFQPPDLET
     LFYAQPRREA CA