SMRA_ECOLI
ID SMRA_ECOLI Reviewed; 187 AA.
AC P76053; Q2MBF1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Probable DNA endonuclease SmrA;
DE EC=3.1.-.-;
GN Name=smrA; Synonyms=ydaL; OrderedLocusNames=b1340, JW1334;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 39-175, PROTEIN SEQUENCE OF 39-45,
RP DNA-BINDING, AND FUNCTION.
RX PubMed=21276852; DOI=10.1016/j.jsb.2011.01.008;
RA Gui W.J., Qu Q.H., Chen Y.Y., Wang M., Zhang X.E., Bi L.J., Jiang T.;
RT "Crystal structure of YdaL, a stand-alone small MutS-related protein from
RT Escherichia coli.";
RL J. Struct. Biol. 174:282-289(2011).
CC -!- FUNCTION: Has DNA endonuclease activity. Binds DNA.
CC {ECO:0000269|PubMed:21276852}.
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DR EMBL; U00096; AAC74422.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76405.1; -; Genomic_DNA.
DR PIR; G64883; G64883.
DR RefSeq; NP_415856.1; NC_000913.3.
DR RefSeq; WP_001046829.1; NZ_SSZK01000012.1.
DR PDB; 3QD7; X-ray; 2.30 A; X=39-175.
DR PDBsum; 3QD7; -.
DR AlphaFoldDB; P76053; -.
DR SMR; P76053; -.
DR BioGRID; 4262880; 6.
DR IntAct; P76053; 1.
DR STRING; 511145.b1340; -.
DR jPOST; P76053; -.
DR PaxDb; P76053; -.
DR PRIDE; P76053; -.
DR EnsemblBacteria; AAC74422; AAC74422; b1340.
DR EnsemblBacteria; BAE76405; BAE76405; BAE76405.
DR GeneID; 67417350; -.
DR GeneID; 945953; -.
DR KEGG; ecj:JW1334; -.
DR KEGG; eco:b1340; -.
DR PATRIC; fig|1411691.4.peg.937; -.
DR EchoBASE; EB3137; -.
DR eggNOG; COG2840; Bacteria.
DR HOGENOM; CLU_055978_1_2_6; -.
DR InParanoid; P76053; -.
DR OMA; MIKSHVN; -.
DR PhylomeDB; P76053; -.
DR BioCyc; EcoCyc:G6672-MON; -.
DR EvolutionaryTrace; P76053; -.
DR PRO; PR:P76053; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:EcoCyc.
DR Gene3D; 3.30.1370.110; -; 1.
DR InterPro; IPR002625; Smr_dom.
DR InterPro; IPR036063; Smr_dom_sf.
DR Pfam; PF01713; Smr; 1.
DR SMART; SM00463; SMR; 1.
DR SUPFAM; SSF160443; SSF160443; 1.
DR PROSITE; PS50828; SMR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-binding; Endonuclease;
KW Hydrolase; Nuclease; Reference proteome.
FT CHAIN 1..187
FT /note="Probable DNA endonuclease SmrA"
FT /id="PRO_0000168906"
FT DOMAIN 88..169
FT /note="Smr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00321"
FT CONFLICT 40
FT /note="D -> E (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="D -> E (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 43..45
FT /evidence="ECO:0007829|PDB:3QD7"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:3QD7"
FT TURN 71..74
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 75..78
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:3QD7"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 95..111
FT /evidence="ECO:0007829|PDB:3QD7"
FT STRAND 115..120
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 130..143
FT /evidence="ECO:0007829|PDB:3QD7"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 156..158
FT /evidence="ECO:0007829|PDB:3QD7"
FT HELIX 160..162
FT /evidence="ECO:0007829|PDB:3QD7"
FT STRAND 163..168
FT /evidence="ECO:0007829|PDB:3QD7"
SQ SEQUENCE 187 AA; 21512 MW; 5FC4F67116D92129 CRC64;
MNLDDKSLFL DAMEDVQPLK RATDVHWHPT RNQRAPQRID TLQLDNFLTT GFLDIIPLSQ
PLEFRREGLQ HGVLDKLRSG KYPQQASLNL LRQPVEECRK MVFSFIQQAL ADGLRNVLII
HGKGRDDKSH ANIVRSYVAR WLTEFDDVQA YCTALPHHGG SGACYVALRK TAQAKQENWE
RHAKRSR
