SMRP1_MOUSE
ID SMRP1_MOUSE Reviewed; 260 AA.
AC Q2MH31; Q0GC36; Q9D1B3;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Spermatid-specific manchette-related protein 1;
DE AltName: Full=Ciliated bronchial epithelial protein 1;
GN Name=Smrp1; Synonyms=Cbe1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH
RP ALPHA-TUBULIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Testis;
RX PubMed=18163442; DOI=10.1002/mrd.20835;
RA Matsuoka Y., Miyagawa Y., Tokuhiro K., Kitamura K., Iguchi N., Maekawa M.,
RA Takahashi T., Tsujimura A., Matsumiya K., Okuyama A., Nishimune Y.,
RA Tanaka H.;
RT "Isolation and characterization of the spermatid-specific Smrp1 gene
RT encoding a novel manchette protein.";
RL Mol. Reprod. Dev. 75:967-975(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING
RP (ISOFORMS 1; 2; 3 AND 4), TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=MF1;
RX PubMed=19213785; DOI=10.1183/09031936.00157108;
RA Haitchi H.M., Yoshisue H., Ribbene A., Wilson S.J., Holloway J.W.,
RA Bucchieri F., Hanley N.A., Wilson D.I., Zummo G., Holgate S.T.,
RA Davies D.E.;
RT "Chronological expression of Ciliated Bronchial Epithelium 1 during
RT pulmonary development.";
RL Eur. Respir. J. 33:1095-1104(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: May be involved in differentiation or function of ciliated
CC cells (By similarity). May play a role in spermatogenesis.
CC {ECO:0000250, ECO:0000269|PubMed:18163442}.
CC -!- SUBUNIT: Interacts with alpha-tubulin. {ECO:0000269|PubMed:18163442}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:18163442}. Nucleus {ECO:0000250}. Cytoplasm
CC {ECO:0000250}. Note=Localizes to the manchette in elongating
CC spermatids. Predominantly perinuclear in bronchial epithelial cells but
CC also detected in the nucleus in some primary epithelial cells and in a
CC number of cell lines.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=Cbe1-long-ORF1;
CC IsoId=Q2MH31-1; Sequence=Displayed;
CC Name=2; Synonyms=Cbe1-long-ORF2;
CC IsoId=Q2MH31-4; Sequence=VSP_027173;
CC Name=3; Synonyms=Cbe1-ORF1;
CC IsoId=Q2MH31-2; Sequence=VSP_027172;
CC Name=4; Synonyms=Cbe1-ORF2;
CC IsoId=Q2MH31-3; Sequence=VSP_027172, VSP_027173;
CC -!- TISSUE SPECIFICITY: Isoforms 1 is testis-specific where it is expressed
CC exclusively in germ cells (at protein level). Isoform 2 is testis-
CC specific. Isoform 3 and isoform 4 are expressed in both lung and
CC testis. {ECO:0000269|PubMed:18163442, ECO:0000269|PubMed:19213785}.
CC -!- DEVELOPMENTAL STAGE: During male germ cell development, expression of
CC isoform 1 begins at day 21, increases after day 30 and continues to
CC increase in the adult. Expressed in elongating spermatids from steps 9-
CC 12 (at protein level). Expression in the lung increases at 16 dpc.
CC {ECO:0000269|PubMed:18163442, ECO:0000269|PubMed:19213785}.
CC -!- SIMILARITY: Belongs to the SMRP1 family. {ECO:0000305}.
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DR EMBL; AB231294; BAE78528.1; -; mRNA.
DR EMBL; DQ873295; ABI35956.1; -; mRNA.
DR EMBL; DQ873296; ABI35957.1; -; mRNA.
DR EMBL; AK003742; BAB22974.1; -; mRNA.
DR EMBL; AL831723; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS38717.1; -. [Q2MH31-1]
DR RefSeq; NP_001041470.1; NM_001048005.1. [Q2MH31-1]
DR RefSeq; NP_001240706.1; NM_001253777.1. [Q2MH31-4]
DR RefSeq; NP_001240707.1; NM_001253778.1. [Q2MH31-3]
DR RefSeq; NP_082900.1; NM_028624.1. [Q2MH31-2]
DR AlphaFoldDB; Q2MH31; -.
DR STRING; 10090.ENSMUSP00000030152; -.
DR iPTMnet; Q2MH31; -.
DR PhosphoSitePlus; Q2MH31; -.
DR PaxDb; Q2MH31; -.
DR PRIDE; Q2MH31; -.
DR ProteomicsDB; 261455; -. [Q2MH31-1]
DR ProteomicsDB; 261457; -. [Q2MH31-2]
DR ProteomicsDB; 261458; -. [Q2MH31-3]
DR TopDownProteomics; Q2MH31-1; -. [Q2MH31-1]
DR Antibodypedia; 55618; 21 antibodies from 13 providers.
DR Ensembl; ENSMUST00000030152; ENSMUSP00000030152; ENSMUSG00000028441. [Q2MH31-1]
DR GeneID; 73721; -.
DR KEGG; mmu:73721; -.
DR UCSC; uc008siu.1; mouse. [Q2MH31-2]
DR UCSC; uc008siv.1; mouse. [Q2MH31-3]
DR UCSC; uc008six.1; mouse. [Q2MH31-1]
DR UCSC; uc008siy.1; mouse. [Q2MH31-4]
DR MGI; MGI:1920971; 1110017D15Rik.
DR VEuPathDB; HostDB:ENSMUSG00000028441; -.
DR eggNOG; ENOG502QRFQ; Eukaryota.
DR GeneTree; ENSGT00390000000945; -.
DR HOGENOM; CLU_093197_0_0_1; -.
DR InParanoid; Q2MH31; -.
DR OMA; ANKFVTR; -.
DR OrthoDB; 942401at2759; -.
DR PhylomeDB; Q2MH31; -.
DR TreeFam; TF335656; -.
DR BioGRID-ORCS; 73721; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q2MH31; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q2MH31; protein.
DR Bgee; ENSMUSG00000028441; Expressed in seminiferous tubule of testis and 117 other tissues.
DR ExpressionAtlas; Q2MH31; baseline and differential.
DR Genevisible; Q2MH31; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0002177; C:manchette; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
DR GO; GO:0043014; F:alpha-tubulin binding; IDA:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0065003; P:protein-containing complex assembly; IDA:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR028195; SMRP1.
DR PANTHER; PTHR35664; PTHR35664; 1.
DR Pfam; PF15181; SMRP1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Differentiation; Nucleus;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..260
FT /note="Spermatid-specific manchette-related protein 1"
FT /id="PRO_0000296260"
FT VAR_SEQ 1..134
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072,
FT ECO:0000303|PubMed:19213785"
FT /id="VSP_027172"
FT VAR_SEQ 233..260
FT /note="ECVALRSPARNAMCCYNSPAIILPVSQP -> VRSVSPFGHRPGMQCAVTTP
FT PPSFYPYPNLRWDTSHFKKTGGSQRNNYVVHPEFVSETCPVYPS (in isoform 2
FT and isoform 4)"
FT /evidence="ECO:0000303|PubMed:19213785"
FT /id="VSP_027173"
SQ SEQUENCE 260 AA; 29939 MW; 3BAE6877F4490A0E CRC64;
MFLFSRKTKT PISTYSDSYR APTSIKEVYK DPPLWAWEAN KFVTPGLTQT MHRHVDPEAL
QKMTKCAAQD YTYKSSISGH PYLPEKYWLS PDEEDKCCPS YLDNDRYNTW KTSPCSNYWN
KYTGCLPRLS KDTGMESVRG MPLEYPPKQE RLNAYEREVV VNMLNSLSRN RTLPQIVPRC
GCVDPLPGRL PYQGYESPCS GRHYCLRGMD YCTTREPSTE RRLRPLCSQQ PTECVALRSP
ARNAMCCYNS PAIILPVSQP
