ID   SMS2_ICTPU              Reviewed;         105 AA.
AC   P01172;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Somatostatin-2;
DE   AltName: Full=Somatostatin II;
DE   Contains:
DE     RecName: Full=Somatostatin-22;
DE              Short=SS-22;
DE   Flags: Precursor;
GN   Name=sst2;
OS   Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Ictaluridae; Ictalurus.
OX   NCBI_TaxID=7998;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2863931; DOI=10.1007/978-1-4615-7886-4_2;
RA   Dixon J.E., Andrews P.C.;
RT   "Somatostatins of the channel catfish.";
RL   Adv. Exp. Med. Biol. 188:19-29(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6127673; DOI=10.1073/pnas.79.17.5152;
RA   Magazin M.D., Minth C.D., Funckes C.L., Deschenes R.J., Tavianini M.A.,
RA   Dixon J.E.;
RT   "Sequence of a cDNA encoding pancreatic preprosomatostatin-22.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:5152-5156(1982).
RN   [3]
RP   PROTEIN SEQUENCE OF 84-105, GLYCOSYLATION AT THR-88, AND MASS SPECTROMETRY.
RX   PubMed=6149220; DOI=10.1016/s0021-9258(18)90688-5;
RA   Andrews P.C., Pubols M.H., Hermodson M.A., Sheares B.T., Dixon J.E.;
RT   "Structure of the 22-residue somatostatin from catfish. An O-glycosylated
RT   peptide having multiple forms.";
RL   J. Biol. Chem. 259:13267-13272(1984).
RN   [4]
RP   PROTEIN SEQUENCE OF 84-105.
RX   PubMed=7358665; DOI=10.1016/s0021-9258(19)85880-5;
RA   Oyama H., Bradshaw R.A., Bates O.J., Permutt A.;
RT   "Amino acid sequence of catfish pancreatic somatostatin I.";
RL   J. Biol. Chem. 255:2251-2254(1980).
CC   -!- FUNCTION: Somatostatin inhibits the release of somatotropin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Pancreas.
CC   -!- PTM: O-linked glycan consists of Gal-GalNAc disaccharide which is
CC       modified with sialic acid residues (microheterogeneity).
CC       {ECO:0000269|PubMed:6149220}.
CC   -!- MASS SPECTROMETRY: Mass=2943; Mass_error=2; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:6149220};
CC   -!- MISCELLANEOUS: Biological activity of somatostatin-22 on rat pituitary
CC       cells was found to be only 0.01-0.1% of somatostatin-14 activity.
CC   -!- SIMILARITY: Belongs to the somatostatin family. {ECO:0000305}.
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DR   EMBL; M25904; AAA49341.1; -; mRNA.
DR   EMBL; J00945; AAA49340.1; -; mRNA.
DR   PIR; S00293; RIIDS2.
DR   RefSeq; XP_017351534.1; XM_017496045.1.
DR   AlphaFoldDB; P01172; -.
DR   iPTMnet; P01172; -.
DR   GeneID; 100305027; -.
DR   KEGG; ipu:100305027; -.
DR   CTD; 79186; -.
DR   OMA; WKSRTAC; -.
DR   OrthoDB; 1483326at2759; -.
DR   Proteomes; UP000221080; Chromosome 20.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004250; Somatostatin.
DR   InterPro; IPR018142; Somatostatin/Cortistatin_C.
DR   Pfam; PF03002; Somatostatin; 1.
DR   PIRSF; PIRSF001814; Somatostatin; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hormone; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..81
FT                   /id="PRO_0000033126"
FT   PEPTIDE         84..105
FT                   /note="Somatostatin-22"
FT                   /id="PRO_0000033127"
FT   CARBOHYD        88
FT                   /note="O-linked (GalNAc...) threonine"
FT                   /evidence="ECO:0000269|PubMed:6149220"
FT   DISULFID        94..105
FT                   /evidence="ECO:0000250|UniProtKB:P61278"
FT   CONFLICT        88
FT                   /note="T -> R (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        102
FT                   /note="R -> S (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   105 AA;  11769 MW;  E4948F90853AC57C CRC64;
     MSSSPLRLAL ALMCLVSAVG VISCGRPHVV LNSALEEARN VPFGEEVPER LTLPELQWML
     SNNELTPVQV EEAPRSRLEL VRRDNTVTSK PLNCMNYFWK SRTAC