ID   SMSY_SANMU              Reviewed;         569 AA.
AC   F6M8H8;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Probable santalene synthase;
DE            Short=SmSSY;
DE            EC=4.2.3.-;
GN   Name=SSY;
OS   Santalum murrayanum (Bitter quandong).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=453085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Catalyzes the formation of santalene. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- MISCELLANEOUS: The oil-deficient phenotype of this species is not due
CC       to the absence of the genes encoding enzymes for santalene
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JF746811; AEF32533.1; -; Genomic_DNA.
DR   AlphaFoldDB; F6M8H8; -.
DR   SMR; F6M8H8; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..569
FT                   /note="Probable santalene synthase"
FT                   /id="PRO_0000419329"
FT   MOTIF           321..325
FT                   /note="DDXXD motif"
FT   BINDING         321
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         321
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         325
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         325
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         467
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         471
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   569 AA;  65307 MW;  198FFF036D46F496 CRC64;
     MDSSTATAMT APFIDPTDHV NLKTDTDASE NRRMGNYKPS IWNYDFLQSL ATHHNIVEER
     HLKLAEKLKG QVKFMFGAPM EPLAKLELVD VVQRLGLNHL FETEIKEVLF SIYKDGSNGW
     WFDHLHATSL RFRLLRQCGL FIPQDVFKMF QNKTGELDMK LCDNVKGLLS LYEASYLGWK
     GENILDEAKA FATKYLKSAW ENISEKWLAK RVKHALALPL HWRVPRIEAR WFIEAYEQEA
     NMNPTLLKLA KLDFNMVQSI HQKEIGELAR WWVTTGLDKL AFARNNLLQS YMWSCAIASD
     PKFKLARETI VEIGSVLTVV DDAYDVYGSM DELDLYTSSV ERWSCVEIDK LPNTLKLIFM
     SMFNKTNEVG LRVQHERGYN SIPTFIKAWV QQCKSYQKEA RWFHGGHTPP LEEYSLNGLV
     SIGFPLLLIT GYVAIAENEA ALDKVHPLPD LLHYSSLLSR LINDIGTSPD EMARGDNLKS
     IHCYMNETGA SEEVAREHIK GVIEENWKIL NQCCFDQSQF QEPFITFNLN SVRGSHFFYE
     FGDGFGVTDS WTKVDMKSVL IDPIPLGEE