ID   SMT1_ORYSJ              Reviewed;         344 AA.
AC   Q6ZIX2; O82426; Q0D7V5;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Cycloartenol-C-24-methyltransferase 1;
DE            EC=2.1.1.41;
DE   AltName: Full=24-sterol C-methyltransferase 1;
DE            Short=Sterol C-methyltransferase 1;
GN   Name=Smt1-1; OrderedLocusNames=Os07g0206700, LOC_Os07g10600;
GN   ORFNames=OJ1119_B04.19-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Root;
RX   PubMed=9746350; DOI=10.1046/j.1432-1327.1998.2560088.x;
RA   Bouvier-Nave P., Husselstein T., Benveniste P.;
RT   "Two families of sterol methyltransferases are involved in the first and
RT   the second methylation steps of plant sterol biosynthesis.";
RL   Eur. J. Biochem. 256:88-96(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the C-24 of cycloartenol to form 24-methylene cycloartenol.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + zymosterol = fecosterol + H(+) + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:21128, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17038, ChEBI:CHEBI:18252, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789; EC=2.1.1.41;
CC   -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR   EMBL; AF042332; AAC34988.1; -; mRNA.
DR   EMBL; AP003943; BAC83238.1; -; Genomic_DNA.
DR   EMBL; AP008213; BAF21068.1; -; Genomic_DNA.
DR   EMBL; AP014963; BAT00552.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6ZIX2; -.
DR   SMR; Q6ZIX2; -.
DR   IntAct; Q6ZIX2; 2.
DR   STRING; 4530.OS07T0206700-01; -.
DR   PaxDb; Q6ZIX2; -.
DR   PRIDE; Q6ZIX2; -.
DR   EnsemblPlants; Os07t0206700-01; Os07t0206700-01; Os07g0206700.
DR   Gramene; Os07t0206700-01; Os07t0206700-01; Os07g0206700.
DR   eggNOG; KOG1269; Eukaryota.
DR   InParanoid; Q6ZIX2; -.
DR   OMA; ISNMCKV; -.
DR   BioCyc; MetaCyc:MON-7706; -.
DR   PlantReactome; R-OSA-1119370; Sterol biosynthesis.
DR   UniPathway; UPA00766; -.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   ExpressionAtlas; Q6ZIX2; baseline and differential.
DR   Genevisible; Q6ZIX2; OS.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0003838; F:sterol 24-C-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   2: Evidence at transcript level;
KW   Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT   CHAIN           1..344
FT                   /note="Cycloartenol-C-24-methyltransferase 1"
FT                   /id="PRO_0000124803"
FT   CONFLICT        299
FT                   /note="Y -> YKALEY (in Ref. 1; AAC34988)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   344 AA;  38612 MW;  1510C2F576B47ADB CRC64;
     MSRSGAMDLA SGLGGKITKD EVKSAVDEYE KYHGYYGGKE EARKSNYTDM VNKYYDLATS
     FYEYGWGESF HFAHRWNGES LRESIKRHEH FLALQLGVKP GMKVLDVGCG IGGPLREIAK
     FSLASVTGLN NNEYQITRGK ELNRVAGVSG TCDFVKADFM KMPFSDNTFD AVYAIEATCH
     APDPVGCYKE IYRVLKPGQC FAVYEWCITD HYEPNNATHK RIKDEIELGN GLPDIRSTQQ
     CLQAAKDAGF EVIWDKDLAE DSPVPWYLPL DPSRFSLSSF RLTTVGRAIT RTMVKALEYV
     GLAPQGSERV SNFLEKAAEG LVEGGKKEIF TPMYFFLVRK PISE