ID   SMT2_ORYSJ              Reviewed;         363 AA.
AC   O82427;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=24-methylenesterol C-methyltransferase 2;
DE            Short=24-sterol C-methyltransferase 2;
DE            Short=Sterol-C-methyltransferase 2;
DE            EC=2.1.1.143;
GN   Name=Smt2-1; OrderedLocusNames=Os03g0136200, LOC_Os03g04340;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Callus;
RX   PubMed=9746350; DOI=10.1046/j.1432-1327.1998.2560088.x;
RA   Bouvier-Nave P., Husselstein T., Benveniste P.;
RT   "Two families of sterol methyltransferases are involved in the first and
RT   the second methylation steps of plant sterol biosynthesis.";
RL   Eur. J. Biochem. 256:88-96(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the methylene group of 24-methylene lophenol to form 24-ethylidene
CC       lophenol. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=24-methylidenelophenol + S-adenosyl-L-methionine = (Z)-24-
CC         ethylidenelophenol + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:21044, ChEBI:CHEBI:15378, ChEBI:CHEBI:29107,
CC         ChEBI:CHEBI:33203, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789;
CC         EC=2.1.1.143;
CC   -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR   EMBL; AF042333; AAC34989.1; -; mRNA.
DR   EMBL; AC118132; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP014959; BAS82182.1; -; Genomic_DNA.
DR   RefSeq; XP_015630743.1; XM_015775257.1.
DR   AlphaFoldDB; O82427; -.
DR   SMR; O82427; -.
DR   STRING; 4530.OS03T0136200-01; -.
DR   PaxDb; O82427; -.
DR   PRIDE; O82427; -.
DR   EnsemblPlants; Os03t0136200-01; Os03t0136200-01; Os03g0136200.
DR   GeneID; 4331540; -.
DR   Gramene; Os03t0136200-01; Os03t0136200-01; Os03g0136200.
DR   KEGG; osa:4331540; -.
DR   eggNOG; KOG1269; Eukaryota.
DR   HOGENOM; CLU_039068_5_2_1; -.
DR   InParanoid; O82427; -.
DR   OMA; EYFQHWD; -.
DR   OrthoDB; 661953at2759; -.
DR   PlantReactome; R-OSA-1119370; Sterol biosynthesis.
DR   UniPathway; UPA00766; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; O82427; baseline and differential.
DR   Genevisible; O82427; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030797; F:24-methylenesterol C-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR044625; SMT2/3-like.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   PANTHER; PTHR44742; PTHR44742; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   2: Evidence at transcript level;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..363
FT                   /note="24-methylenesterol C-methyltransferase 2"
FT                   /id="PRO_0000124804"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        214
FT                   /note="D -> E (in Ref. 1; AAC34989)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        284
FT                   /note="L -> V (in Ref. 1; AAC34989)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        291..292
FT                   /note="AL -> GF (in Ref. 1; AAC34989)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   363 AA;  40619 MW;  ED7398EAC1D1B000 CRC64;
     MEAATMAWTA AGVGMALVYW FVWVMGAAEV KGKRAVDLKM GSITNDKVKD KYTQYWSFFR
     RPKETATTEA SAEKVPAFVD TFYNLVTDIY EWGWGQSFHF SPSLPGRSHR EATRVHEERV
     ADLLQAKPGH RLLDVGCGVG GPMRAIAAHS GSNVVGITIN EYQVNRARAH NRKAGLDSRC
     EVVCGNFLSM PFSDASFDGA YSIEATCHAP RLQDVYGEVF RVLKPGGLYV SYEWVTTSLY
     RADNPEHVEA IHGIERGDAL PGLRRQDEIA SIAKEVGFEV LKELDLALPP ALPWWTRLKM
     GRIAYWRNSL VVRVLTMLRI APKGVCEVHE MLYETAQHLT RGGETGIFTP MHMVLLRKPV
     ESK