ID   SMT3B_ARATH             Reviewed;         359 AA.
AC   Q94JS4; O23655;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=24-methylenesterol C-methyltransferase 3;
DE            Short=24-sterol C-methyltransferase 3;
DE            Short=Sterol-C-methyltransferase 3;
DE            EC=2.1.1.143;
GN   Name=SMT3; OrderedLocusNames=At1g76090; ORFNames=T23E18.40;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Seedling;
RX   PubMed=9208946; DOI=10.1111/j.1432-1033.1997.t01-1-00518.x;
RA   Bouvier-Nave P., Husselstein T., Desprez T., Benveniste P.;
RT   "Identification of cDNAs encoding sterol methyl-transferases involved in
RT   the second methylation step of plant sterol biosynthesis.";
RL   Eur. J. Biochem. 246:518-529(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the methylene group of 24-methylene lophenol to form 24-ethylidene
CC       lophenol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=24-methylidenelophenol + S-adenosyl-L-methionine = (Z)-24-
CC         ethylidenelophenol + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:21044, ChEBI:CHEBI:15378, ChEBI:CHEBI:29107,
CC         ChEBI:CHEBI:33203, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789;
CC         EC=2.1.1.143;
CC   -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR   EMBL; U71400; AAB62809.1; -; mRNA.
DR   EMBL; AC009978; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002684; AEE35795.1; -; Genomic_DNA.
DR   EMBL; AF375397; AAK52981.1; -; mRNA.
DR   EMBL; AY113031; AAM47339.1; -; mRNA.
DR   RefSeq; NP_177736.1; NM_106258.4.
DR   AlphaFoldDB; Q94JS4; -.
DR   SMR; Q94JS4; -.
DR   BioGRID; 29160; 1.
DR   STRING; 3702.AT1G76090.1; -.
DR   PaxDb; Q94JS4; -.
DR   PRIDE; Q94JS4; -.
DR   ProteomicsDB; 245319; -.
DR   EnsemblPlants; AT1G76090.1; AT1G76090.1; AT1G76090.
DR   GeneID; 843941; -.
DR   Gramene; AT1G76090.1; AT1G76090.1; AT1G76090.
DR   KEGG; ath:AT1G76090; -.
DR   Araport; AT1G76090; -.
DR   TAIR; locus:2199777; AT1G76090.
DR   eggNOG; KOG1269; Eukaryota.
DR   HOGENOM; CLU_039068_5_2_1; -.
DR   InParanoid; Q94JS4; -.
DR   OMA; DWVITDK; -.
DR   OrthoDB; 661953at2759; -.
DR   PhylomeDB; Q94JS4; -.
DR   BioCyc; ARA:AT1G76090-MON; -.
DR   BioCyc; MetaCyc:AT1G76090-MON; -.
DR   UniPathway; UPA00766; -.
DR   PRO; PR:Q94JS4; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q94JS4; baseline and differential.
DR   Genevisible; Q94JS4; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030797; F:24-methylenesterol C-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003838; F:sterol 24-C-methyltransferase activity; TAS:TAIR.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; TAS:TAIR.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR044625; SMT2/3-like.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   PANTHER; PTHR44742; PTHR44742; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   2: Evidence at transcript level;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..359
FT                   /note="24-methylenesterol C-methyltransferase 3"
FT                   /id="PRO_0000124802"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        84
FT                   /note="I -> T (in Ref. 1; AAB62809)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   359 AA;  40124 MW;  3C5B6B53CE622989 CRC64;
     MDSVALYCTA GLIAGAVYWF ICVLGPAERK GKRASDLSGG SISAEKVKDN YNQYWSFFRK
     PKEIESAEKV PDFVDTFYNL VTDIYEWGWG QSFHFSPHVP GKSDKDATRI HEEMAVDLIK
     VKPGQKILDA GCGVGGPMRA IAAHSKAQVT GITINEYQVQ RAKLHNKKAG LDSLCNVVCG
     NFLKMPFDEN TFDGAYSIEA TCHAPKLEEV YSEIFRVMKP GSLFVSYEWV TTEKYRDDDE
     EHKDVIQGIE RGDALPGLRS YADIAVTAKK VGFEVVKEKD LAKPPSKPWW NRLKMGRIAY
     WRNHVVVVIL SAIGVAPKGT VDVHKMLFKT ADYLTRGGET GIFSPMHMIL CRKPEKASE