SMTN_MOUSE
ID SMTN_MOUSE Reviewed; 923 AA.
AC Q921U8; Q3TCV2; Q6NSW1; Q8CD93; Q9JHG8; Q9JLU7; Q9R0D0; Q9R253; Q9Z0Q2;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Smoothelin;
GN Name=Smtn; Synonyms=Smsmo;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=L129;
RX PubMed=10965129; DOI=10.1159/000015619;
RA Rensen S., Merkx G., Doevendans P., Geurts Van Kessel A., van Eys G.J.J.M.;
RT "Structure and chromosome location of Smtn, the mouse smoothelin gene.";
RL Cytogenet. Cell Genet. 89:225-229(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L1 AND L2).
RC STRAIN=129; TISSUE=Heart;
RX PubMed=10023782; DOI=10.1007/s001090050352;
RA Kraemer J., Aguirre-Arteta A.M., Thiel C., Gross M.C., Dietz R.,
RA Cardoso M.C., Leonhardt H.;
RT "A novel isoform of the smooth muscle cell differentiation marker
RT smoothelin.";
RL J. Mol. Med. 77:294-298(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=129;
RA Kraemer J., Cardoso M.C., Gross C.M., Dietz R., Leonhardt H.;
RT "Mouse smoothelin gene structure and its splicing variants.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Blastocyst, Embryo, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Structural protein of the cytoskeleton. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC Note=Exhibits a filamentous organization. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=L1; Synonyms=B;
CC IsoId=Q921U8-1; Sequence=Displayed;
CC Name=L2;
CC IsoId=Q921U8-2; Sequence=VSP_031244;
CC Name=S1; Synonyms=A;
CC IsoId=Q921U8-3; Sequence=VSP_031243;
CC Name=S2;
CC IsoId=Q921U8-4; Sequence=VSP_031243, VSP_031244;
CC -!- SIMILARITY: Belongs to the smoothelin family. {ECO:0000305}.
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DR EMBL; AF218834; AAD29628.2; -; Genomic_DNA.
DR EMBL; AF116517; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF116518; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF195524; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF195525; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218829; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218830; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218831; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218832; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218833; AAD29628.2; JOINED; Genomic_DNA.
DR EMBL; AF218834; AAF67392.1; -; Genomic_DNA.
DR EMBL; AF116518; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF195525; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF218830; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF218831; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF218832; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF218833; AAF67392.1; JOINED; Genomic_DNA.
DR EMBL; AF064236; AAF01480.1; -; mRNA.
DR EMBL; AJ010305; CAA09076.1; -; mRNA.
DR EMBL; AF132449; AAF25577.2; -; Genomic_DNA.
DR EMBL; AF132449; AAF25578.2; -; Genomic_DNA.
DR EMBL; AF132449; AAF25579.2; -; Genomic_DNA.
DR EMBL; AF132449; AAF25580.2; -; Genomic_DNA.
DR EMBL; AK030932; BAC27188.1; -; mRNA.
DR EMBL; AK170516; BAE41853.1; -; mRNA.
DR EMBL; BC010599; AAH10599.1; -; mRNA.
DR EMBL; BC066192; AAH66192.1; -; mRNA.
DR EMBL; BC069836; AAH69836.1; -; mRNA.
DR EMBL; BC069839; AAH69839.1; -; mRNA.
DR CCDS; CCDS24366.1; -. [Q921U8-2]
DR CCDS; CCDS70129.1; -. [Q921U8-1]
DR RefSeq; NP_001152756.1; NM_001159284.1. [Q921U8-2]
DR RefSeq; NP_001271356.1; NM_001284427.1. [Q921U8-1]
DR RefSeq; NP_001271357.1; NM_001284428.1. [Q921U8-2]
DR RefSeq; NP_001271358.1; NM_001284429.1.
DR RefSeq; NP_038898.2; NM_013870.3. [Q921U8-2]
DR RefSeq; XP_017170075.1; XM_017314586.1. [Q921U8-1]
DR AlphaFoldDB; Q921U8; -.
DR SMR; Q921U8; -.
DR BioGRID; 205923; 4.
DR IntAct; Q921U8; 2.
DR STRING; 10090.ENSMUSP00000020721; -.
DR iPTMnet; Q921U8; -.
DR PhosphoSitePlus; Q921U8; -.
DR EPD; Q921U8; -.
DR MaxQB; Q921U8; -.
DR PaxDb; Q921U8; -.
DR PeptideAtlas; Q921U8; -.
DR PRIDE; Q921U8; -.
DR ProteomicsDB; 261378; -. [Q921U8-1]
DR ProteomicsDB; 261379; -. [Q921U8-2]
DR ProteomicsDB; 261380; -. [Q921U8-3]
DR ProteomicsDB; 261381; -. [Q921U8-4]
DR Antibodypedia; 3445; 177 antibodies from 24 providers.
DR DNASU; 29856; -.
DR Ensembl; ENSMUST00000020721; ENSMUSP00000020721; ENSMUSG00000020439. [Q921U8-2]
DR Ensembl; ENSMUST00000075118; ENSMUSP00000074621; ENSMUSG00000020439. [Q921U8-1]
DR Ensembl; ENSMUST00000170588; ENSMUSP00000133155; ENSMUSG00000020439. [Q921U8-2]
DR GeneID; 29856; -.
DR KEGG; mmu:29856; -.
DR UCSC; uc007htf.2; mouse. [Q921U8-2]
DR UCSC; uc007hti.2; mouse. [Q921U8-1]
DR CTD; 6525; -.
DR MGI; MGI:1354727; Smtn.
DR VEuPathDB; HostDB:ENSMUSG00000020439; -.
DR eggNOG; KOG4678; Eukaryota.
DR GeneTree; ENSGT00940000161655; -.
DR HOGENOM; CLU_014660_0_0_1; -.
DR InParanoid; Q921U8; -.
DR OMA; KTPYPGF; -.
DR OrthoDB; 168604at2759; -.
DR TreeFam; TF316716; -.
DR BioGRID-ORCS; 29856; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Smtn; mouse.
DR PRO; PR:Q921U8; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q921U8; protein.
DR Bgee; ENSMUSG00000020439; Expressed in aorta tunica media and 167 other tissues.
DR ExpressionAtlas; Q921U8; baseline and differential.
DR Genevisible; Q921U8; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0031941; C:filamentous actin; IBA:GO_Central.
DR GO; GO:0005815; C:microtubule organizing center; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; TAS:MGI.
DR GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0003085; P:negative regulation of systemic arterial blood pressure; IMP:MGI.
DR GO; GO:0060452; P:positive regulation of cardiac muscle contraction; IMP:MGI.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR022189; SMTN.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF12510; Smoothelin; 3.
DR SMART; SM00033; CH; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT CHAIN 2..923
FT /note="Smoothelin"
FT /id="PRO_0000318593"
FT DOMAIN 805..912
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REGION 134..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 542..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 615..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 24..89
FT /evidence="ECO:0000255"
FT COILED 601..628
FT /evidence="ECO:0000255"
FT COMPBIAS 161..188
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..253
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..322
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 363..389
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 615..637
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..724
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..758
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 301
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 304
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 340
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 359
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 372
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 501
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 521
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 574
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 641
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 735
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT MOD_RES 798
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53814"
FT VAR_SEQ 1..454
FT /note="Missing (in isoform S1 and isoform S2)"
FT /evidence="ECO:0000305"
FT /id="VSP_031243"
FT VAR_SEQ 875..923
FT /note="MLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV ->
FT THADCPQLLDTEDMVRLREPDWKCVYTYIQEFYRCLVQKGLVKTKKS (in isoform
FT L2 and isoform S2)"
FT /evidence="ECO:0000303|PubMed:10023782,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:16141072"
FT /id="VSP_031244"
FT CONFLICT 173
FT /note="P -> S (in Ref. 1; AAD29628, 2; AAF01480/CAA09076
FT and 3; AAF25577/AAF25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 379
FT /note="Missing (in Ref. 5; AAH69839/AAH69836/AAH66192)"
FT /evidence="ECO:0000305"
FT CONFLICT 441
FT /note="P -> Q (in Ref. 4; BAE41853)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="T -> N (in Ref. 4; BAC27188)"
FT /evidence="ECO:0000305"
FT CONFLICT 450
FT /note="E -> G (in Ref. 5; AAH69839/AAH69836/AAH66192)"
FT /evidence="ECO:0000305"
FT CONFLICT 711
FT /note="S -> N (in Ref. 1; AAD29628/AAF67392, 2; AAF01480/
FT CAA09076 and 3; AAF25577/AAF25578/AAF25579/AAF25580)"
FT /evidence="ECO:0000305"
FT CONFLICT 756
FT /note="M -> V (in Ref. 5; AAH69839/AAH69836/AAH66192)"
FT /evidence="ECO:0000305"
FT CONFLICT 837
FT /note="G -> R (in Ref. 1; AAD29628/AAF67392, 2; AAF01480/
FT CAA09076 and 3; AAF25577/AAF25578/AAF25579/AAF25580)"
FT /evidence="ECO:0000305"
FT MOD_RES Q921U8-2:372
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 923 AA; 100289 MW; 2E70E413C8B487C7 CRC64;
MADEALAGLD EGALRKLLEV TADLAERRRI RSAIRELQRQ ELEREEEALA SKRFRAERQD
NKENWLHSQQ REAEQQAALA RLAGRLESMN DVEELTTLLR SAGEYEERKL IRAAIRRVRA
QEIKAATLAG RLCSRLPSSG PREDSRRQAA HTLDPGKVPE PEQQEQQTEV LEPTPTPEDT
SQDVTTVTLL LRAPPGGRPS SPASPHNSPT SASPEPLLEP AGAQCPAVEA PVSSEPLPHP
SEAPSPEPPM SPVPSSSRGR VISKPLPGPT EPSDTLDSIR GFSNTKRADP SETKSCQRSL
SVLSPRQPTP NREPTSLAGP SQFRRVGSVR DRVQKFTSDS PVVARLQDGP PRTALASPTP
TRLPGPSLIS TTPASSSSSN SSSPSPSDTS SHKKQRELAH SLAELQSCPQ EEGPGGRGLA
LRSLENRAGG PKPCSEEPST PPPVAVGTGE PGGSMKTTFT IEIKDGRGQA STGRVLLPTG
NQRAELTLGL RAPPTLLSTS SGGKNTITHI SNPGTVTRLG SVTHVTTFSH ASPGNRGGCN
FKMEPDPAEP PSTTVEAANG AEQARVDKGP EGRSPLSAEE LTAIEDEGVL DKMLDQTTNF
EERKLIRAAL RELRQRKRDQ RDKERERRLR EARARPGESR SNVATETTTR HSQRAADGST
VGTVTKTERL VHSNDGTQTA RTTTVESSFM RRLENGSSSS STTTTTVQTK SFSSSSSSSS
SKKMGSIFDR EDQTSSRPGS LAALERRQAE KKKELMKAQS LPKTSASQAR KAMIEKLEKE
GSAGGPGTPR TAVQRSTSFG VPNANSIKQM LLDWCRAKTR GYEHVDIQNF SSSWSDGMAF
CALVHNFFPE AFDYGQLSPQ NRRQNFEMAF SSAEMLVDCV PLVEVEDMMI MGKKPDPKCV
FTYVQSLYNH LRRHELRLRG KNV
