SMU1_ARATH
ID SMU1_ARATH Reviewed; 511 AA.
AC Q8W117; Q9C9T9;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Suppressor of mec-8 and unc-52 protein homolog 1;
DE Short=AtSMU-1;
DE AltName: Full=RNA splicing protein SMU1;
DE AltName: Full=WD40 repeat-containing protein SMU1;
GN Name=SMU1; OrderedLocusNames=At1g73720; ORFNames=F25P22.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH SMU2, TISSUE SPECIFICITY,
RP AND SUBCELLULAR LOCATION.
RX PubMed=19734266; DOI=10.1104/pp.109.141705;
RA Chung T., Wang D., Kim C.S., Yadegari R., Larkins B.A.;
RT "Plant SMU-1 and SMU-2 homologues regulate pre-mRNA splicing and multiple
RT aspects of development.";
RL Plant Physiol. 151:1498-1512(2009).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Auxiliary spliceosomal protein involved in splicing of
CC specific pre-mRNAs that affect multiple aspects of development.
CC Required for proper accumulation of SMU2.
CC {ECO:0000269|PubMed:19734266}.
CC -!- SUBUNIT: Component of the spliceosome B complex (By similarity).
CC Interacts with SMU2 (PubMed:19734266). {ECO:0000250|UniProtKB:Q2TAY7,
CC ECO:0000269|PubMed:19734266}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19734266}.
CC -!- TISSUE SPECIFICITY: Expressed in actively dividing cells, such as newly
CC emerged leaves and root tips. Detected in embryo, female gametophyte
CC including egg cell, central cell, synergid cells and the antipodal
CC cells, and mature pollen. {ECO:0000269|PubMed:19734266}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous.
CC {ECO:0000269|PubMed:19734266}.
CC -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52064.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012679; AAG52064.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35501.1; -; Genomic_DNA.
DR EMBL; AF462796; AAL58892.1; -; mRNA.
DR EMBL; AY102129; AAM26696.1; -; mRNA.
DR PIR; D96764; D96764.
DR RefSeq; NP_177513.2; NM_106031.4.
DR AlphaFoldDB; Q8W117; -.
DR SMR; Q8W117; -.
DR BioGRID; 28926; 20.
DR IntAct; Q8W117; 3.
DR STRING; 3702.AT1G73720.1; -.
DR iPTMnet; Q8W117; -.
DR PaxDb; Q8W117; -.
DR PRIDE; Q8W117; -.
DR ProteomicsDB; 228449; -.
DR EnsemblPlants; AT1G73720.1; AT1G73720.1; AT1G73720.
DR GeneID; 843707; -.
DR Gramene; AT1G73720.1; AT1G73720.1; AT1G73720.
DR KEGG; ath:AT1G73720; -.
DR Araport; AT1G73720; -.
DR TAIR; locus:2027749; AT1G73720.
DR eggNOG; KOG0275; Eukaryota.
DR HOGENOM; CLU_000288_57_38_1; -.
DR InParanoid; Q8W117; -.
DR OMA; FIEVWNY; -.
DR OrthoDB; 1467963at2759; -.
DR PhylomeDB; Q8W117; -.
DR PRO; PR:Q8W117; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8W117; baseline and differential.
DR Genevisible; Q8W117; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0016607; C:nuclear speck; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0008380; P:RNA splicing; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR045184; SMU1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR22848; PTHR22848; 1.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome; WD repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..511
FT /note="Suppressor of mec-8 and unc-52 protein homolog 1"
FT /id="PRO_0000429841"
FT DOMAIN 6..38
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 40..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 211..252
FT /note="WD 1"
FT REPEAT 261..300
FT /note="WD 2"
FT REPEAT 303..344
FT /note="WD 3"
FT REPEAT 345..384
FT /note="WD 4"
FT REPEAT 393..434
FT /note="WD 5"
FT REPEAT 480..511
FT /note="WD 6"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 511 AA; 57724 MW; 042D5AC50ED94F8A CRC64;
MALEIEARDV IKIMLQFCKE NSLNQTFQTL QSECQVSLNT VDSVETFISD INSGRWDSVL
PQVSQLKLPR NKLEDLYEQI VLEMIELREL DTARAILRQT QVMGVMKQEQ AERYLRMEHL
LVRSYFDPHE AYGDSTKERK RAQIAQAVAA EVTVVPPSRL MALIGQALKW QQHQGLLPPG
TQFDLFRGTA AMKQDVEDTH PNVLTHTIKF GKKSHAECAR FSPDGQFLAS SSVDGFIEVW
DYISGKLKKD LQYQADESFM MHDDPVLCID FSRDSEMLAS GSQDGKIKIW RIRTGVCIRR
FDAHSQGVTS LSFSRDGSQL LSTSFDQTAR IHGLKSGKLL KEFRGHTSYV NHAIFTSDGS
RIITASSDCT VKVWDSKTTD CLQTFKPPPP LRGTDASVNS IHLFPKNTEH IVVCNKTSSI
YIMTLQGQVV KSFSSGNREG GDFVAACVST KGDWIYCIGE DKKLYCFNYQ SGGLEHFMMV
HEKDVIGITH HPHRNLLATY SEDCTMKLWK P
