SMU1_CRIGR
ID SMU1_CRIGR Reviewed; 513 AA.
AC Q76B40;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=WD40 repeat-containing protein SMU1;
DE AltName: Full=Smu-1 suppressor of mec-8 and unc-52 protein homolog;
DE Contains:
DE RecName: Full=WD40 repeat-containing protein SMU1, N-terminally processed;
GN Name=SMU1;
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF GLY-489.
RX PubMed=15878348; DOI=10.1016/j.yexcr.2005.02.017;
RA Sugaya K., Hongo E., Tsuji H.;
RT "A temperature-sensitive mutation in the WD repeat-containing protein Smu1
RT is related to maintenance of chromosome integrity.";
RL Exp. Cell Res. 306:242-251(2005).
RN [2]
RP FUNCTION.
RX PubMed=17105761; DOI=10.1242/jcs.03288;
RA Sugaya K., Hongo E., Ishihara Y., Tsuji H.;
RT "The conserved role of Smu1 in splicing is characterized in its mammalian
RT temperature-sensitive mutant.";
RL J. Cell Sci. 119:4944-4951(2006).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21519198; DOI=10.4161/rna.8.3.14656;
RA Sugaya K., Ishihara Y., Sugaya K.;
RT "Enlargement of speckles of SF2/ASF due to loss of function of Smu1 is
RT characterized in the mammalian temperature-sensitive mutant.";
RL RNA Biol. 8:488-495(2011).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC spliceosome (Probable). Regulates alternative splicing of the HSPG2
CC pre-mRNA (PubMed:17105761). Required for normal accumulation of IK (By
CC similarity). Required for normal mitotic spindle assembly and normal
CC progress through mitosis (PubMed:15878348).
CC {ECO:0000250|UniProtKB:Q2TAY7, ECO:0000269|PubMed:15878348,
CC ECO:0000269|PubMed:17105761, ECO:0000305|PubMed:21519198}.
CC -!- SUBUNIT: Component of the spliceosome B complex. Interacts with IK.
CC {ECO:0000250|UniProtKB:Q2TAY7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99M63}. Nucleus
CC {ECO:0000269|PubMed:21519198}. Nucleus speckle
CC {ECO:0000269|PubMed:21519198}. Note=Colocalizes with SRSF1 in nuclear
CC speckles. {ECO:0000269|PubMed:21519198}.
CC -!- DOMAIN: The WD repeats assemble into a seven-bladed WD propeller.
CC {ECO:0000250|UniProtKB:G5EEG7}.
CC -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
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DR EMBL; AB111945; BAD04854.1; -; mRNA.
DR RefSeq; NP_001231781.1; NM_001244852.1.
DR AlphaFoldDB; Q76B40; -.
DR SMR; Q76B40; -.
DR STRING; 10029.NP_001231781.1; -.
DR GeneID; 100736555; -.
DR KEGG; cge:100736555; -.
DR CTD; 55234; -.
DR eggNOG; KOG0275; Eukaryota.
DR OrthoDB; 1467963at2759; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; IDA:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR045184; SMU1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR22848; PTHR22848; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Isopeptide bond; mRNA processing; mRNA splicing;
KW Nucleus; Repeat; Spliceosome; Ubl conjugation; WD repeat.
FT CHAIN 1..513
FT /note="WD40 repeat-containing protein SMU1"
FT /id="PRO_0000424519"
FT INIT_MET 1
FT /note="Removed; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT CHAIN 2..513
FT /note="WD40 repeat-containing protein SMU1, N-terminally
FT processed"
FT /id="PRO_0000237589"
FT DOMAIN 6..38
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 40..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 212..253
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 262..303
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 305..346
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 347..386
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 395..436
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 440..479
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 482..513
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 2..315
FT /note="Required for interaction with IK"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT MOD_RES 2
FT /note="N-acetylserine; in WD40 repeat-containing protein
FT SMU1, N-terminally processed"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT CROSSLNK 379
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT MUTAGEN 489
FT /note="G->R: Temperature-sensitive mutation in tsTM18 cell
FT line with chromosomal instability and cell cycle arrest at
FT S and G2 phases with decreased DNA synthesis at the
FT nonpermissive temperature of 39 degrees Celsius."
FT /evidence="ECO:0000269|PubMed:15878348"
SQ SEQUENCE 513 AA; 57544 MW; CB201E939DCCA188 CRC64;
MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL
QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL
LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP
GMTIDLFRGK AAVKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV
WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD
GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN
TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL
TVHEKDVIGI AHHPHQNLIA TYSEDGLLKL WKP
