ID   SMU1_DANRE              Reviewed;         513 AA.
AC   Q7ZVA0;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=WD40 repeat-containing protein SMU1;
DE   AltName: Full=Smu-1 suppressor of mec-8 and unc-52 protein homolog;
GN   Name=smu1; ORFNames=zgc:56147;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC       spliceosome (By similarity). Required for normal accumulation of ik (By
CC       similarity). Required for normal mitotic spindle assembly and normal
CC       progress through mitosis (By similarity).
CC       {ECO:0000250|UniProtKB:Q2TAY7, ECO:0000250|UniProtKB:Q76B40}.
CC   -!- SUBUNIT: Component of the spliceosome B complex. Interacts with ik.
CC       {ECO:0000250|UniProtKB:Q2TAY7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99M63}. Nucleus
CC       {ECO:0000250|UniProtKB:Q76B40}. Nucleus speckle
CC       {ECO:0000250|UniProtKB:Q76B40}. Note=Colocalizes with srsf1 in nuclear
CC       speckles. {ECO:0000250|UniProtKB:Q76B40}.
CC   -!- DOMAIN: The WD repeats assemble into a seven-bladed WD propeller.
CC       {ECO:0000250|UniProtKB:G5EEG7}.
CC   -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
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DR   EMBL; BC045945; AAH45945.1; -; mRNA.
DR   RefSeq; NP_956493.1; NM_200199.1.
DR   AlphaFoldDB; Q7ZVA0; -.
DR   SMR; Q7ZVA0; -.
DR   STRING; 7955.ENSDARP00000089117; -.
DR   PaxDb; Q7ZVA0; -.
DR   GeneID; 393168; -.
DR   KEGG; dre:393168; -.
DR   CTD; 393168; -.
DR   ZFIN; ZDB-GENE-040426-916; smu1a.
DR   eggNOG; KOG0275; Eukaryota.
DR   InParanoid; Q7ZVA0; -.
DR   OrthoDB; 1467963at2759; -.
DR   PhylomeDB; Q7ZVA0; -.
DR   PRO; PR:Q7ZVA0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR006595; CTLH_C.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR006594; LisH.
DR   InterPro; IPR045184; SMU1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22848; PTHR22848; 1.
DR   Pfam; PF00400; WD40; 5.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00668; CTLH; 1.
DR   SMART; SM00667; LisH; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50897; CTLH; 1.
DR   PROSITE; PS50896; LISH; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..513
FT                   /note="WD40 repeat-containing protein SMU1"
FT                   /id="PRO_0000237594"
FT   DOMAIN          6..38
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          40..92
FT                   /note="CTLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT   REPEAT          212..253
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          262..303
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          305..346
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          347..386
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          395..436
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          440..479
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          482..513
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REGION          1..315
FT                   /note="Required for interaction with ik"
FT                   /evidence="ECO:0000250|UniProtKB:Q2TAY7"
SQ   SEQUENCE   513 AA;  57531 MW;  A0E11AA1760F3F4B CRC64;
     MSIEIESADV IRLIMQYLKE NSLHRTLAML QEETTVSLNT VDSIESFVAD INSGHWDTVL
     QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL
     LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQSLK WQQHQGLLPP
     GMTIDLFRGK AAVKDVEEER FPTQLARHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV
     WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM SFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
     RYERAHSKGV TCLSFSKDST QILSASFDQT IRIHGLKSGK CLKEFRGHSS FVNEATLTPD
     GHHAISASSD GTVKVWNMKT TECTSTFKSL GTSAGTDITV NNVILLPKNP EHFVVCNRSN
     TVVIMNMQGQ IVRSFSSGKR EGGDFVCCTL SPRGEWIYCV GEDYVLYCFS TVTGKLERTL
     TVHEKDVIGI AHHPHQNLIG TYSEDGLLKL WKP