SMU1_RAT
ID SMU1_RAT Reviewed; 513 AA.
AC Q99M63;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=WD40 repeat-containing protein SMU1;
DE AltName: Full=Brain-enriched WD repeat-containing protein;
DE AltName: Full=Smu-1 suppressor of mec-8 and unc-52 protein homolog;
DE Contains:
DE RecName: Full=WD40 repeat-containing protein SMU1, N-terminally processed;
GN Name=Smu1; Synonyms=Bwd;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11410362; DOI=10.1016/s0378-1119(01)00483-8;
RA Di Benedetto A.J., Klick Stoddard J., Glavan B.J.;
RT "Cloning and molecular characterization of a novel gene encoding a WD-
RT repeat protein expressed in restricted areas of adult rat brain.";
RL Gene 271:21-31(2001).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC spliceosome (By similarity). Regulates alternative splicing of the
CC HSPG2 pre-mRNA (By similarity). Required for normal accumulation of IK
CC (By similarity). Required for normal mitotic spindle assembly and
CC normal progress through mitosis (By similarity).
CC {ECO:0000250|UniProtKB:Q2TAY7, ECO:0000250|UniProtKB:Q76B40}.
CC -!- SUBUNIT: Component of the spliceosome B complex. Interacts with IK.
CC {ECO:0000250|UniProtKB:Q2TAY7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11410362}. Nucleus
CC {ECO:0000269|PubMed:11410362}. Nucleus speckle
CC {ECO:0000250|UniProtKB:Q76B40}. Note=Colocalizes with SRSF1 in nuclear
CC speckles. {ECO:0000250|UniProtKB:Q76B40}.
CC -!- TISSUE SPECIFICITY: Highly expressed in cerebellum, midbrain and heart.
CC Moderately expressed in brain stem, cerebral cortex and liver. Detected
CC in dentate gyrus, pyramidal layer of the hippocampus and Purkinje and
CC granule neurons of cerebellum. {ECO:0000269|PubMed:11410362}.
CC -!- DOMAIN: The WD repeats assemble into a seven-bladed WD propeller.
CC {ECO:0000250|UniProtKB:G5EEG7}.
CC -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY029526; AAK33013.1; -; mRNA.
DR RefSeq; NP_476543.1; NM_057195.1.
DR AlphaFoldDB; Q99M63; -.
DR SMR; Q99M63; -.
DR STRING; 10116.ENSRNOP00000010463; -.
DR jPOST; Q99M63; -.
DR PaxDb; Q99M63; -.
DR GeneID; 117541; -.
DR KEGG; rno:117541; -.
DR UCSC; RGD:620694; rat.
DR CTD; 55234; -.
DR RGD; 620694; Smu1.
DR eggNOG; KOG0275; Eukaryota.
DR InParanoid; Q99M63; -.
DR OrthoDB; 1467963at2759; -.
DR PhylomeDB; Q99M63; -.
DR PRO; PR:Q99M63; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR045184; SMU1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR22848; PTHR22848; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Isopeptide bond; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Repeat; Spliceosome; Ubl conjugation;
KW WD repeat.
FT CHAIN 1..513
FT /note="WD40 repeat-containing protein SMU1"
FT /id="PRO_0000424522"
FT INIT_MET 1
FT /note="Removed; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT CHAIN 2..513
FT /note="WD40 repeat-containing protein SMU1, N-terminally
FT processed"
FT /id="PRO_0000237592"
FT DOMAIN 6..38
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 40..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 212..253
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 262..303
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 305..346
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 347..386
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 395..436
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 440..479
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 482..513
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 2..315
FT /note="Required for interaction with IK"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT MOD_RES 2
FT /note="N-acetylserine; in WD40 repeat-containing protein
FT SMU1, N-terminally processed"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
FT CROSSLNK 379
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
SQ SEQUENCE 513 AA; 57551 MW; 9BE313C1AFA1875A CRC64;
MSIEIESSDV IRLIMQYLKE NSLHRALATF TEETTVSLNT VDSIESFVAD INSGHWDTVL
QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL
LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP
GMTIDLFRGK AAVKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV
WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD
GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN
TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL
TVHEKDVIGI AHHPHQNLIA TYSEDGLLKL WKP
