ID   SMU1_XENLA              Reviewed;         513 AA.
AC   Q6NRT3;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=WD40 repeat-containing protein SMU1;
DE   AltName: Full=Smu-1 suppressor of mec-8 and unc-52 protein homolog;
GN   Name=smu1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC       spliceosome (By similarity). Required for normal accumulation of ik (By
CC       similarity). Required for normal mitotic spindle assembly and normal
CC       progress through mitosis (By similarity).
CC       {ECO:0000250|UniProtKB:Q2TAY7, ECO:0000250|UniProtKB:Q76B40}.
CC   -!- SUBUNIT: Component of the spliceosome B complex. Interacts with ik.
CC       {ECO:0000250|UniProtKB:Q2TAY7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99M63}. Nucleus
CC       {ECO:0000250|UniProtKB:Q76B40}. Nucleus speckle
CC       {ECO:0000250|UniProtKB:Q76B40}. Note=Colocalizes with srsf1 in nuclear
CC       speckles. {ECO:0000250|UniProtKB:Q76B40}.
CC   -!- DOMAIN: The WD repeats assemble into a seven-bladed WD propeller.
CC       {ECO:0000250|UniProtKB:G5EEG7}.
CC   -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
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DR   EMBL; BC070636; AAH70636.1; -; mRNA.
DR   RefSeq; NP_001084865.1; NM_001091396.1.
DR   AlphaFoldDB; Q6NRT3; -.
DR   SMR; Q6NRT3; -.
DR   BioGRID; 101280; 1.
DR   IntAct; Q6NRT3; 3.
DR   MaxQB; Q6NRT3; -.
DR   DNASU; 431914; -.
DR   GeneID; 431914; -.
DR   KEGG; xla:431914; -.
DR   CTD; 431914; -.
DR   Xenbase; XB-GENE-6251564; smu1.S.
DR   OMA; IEIESSX; -.
DR   OrthoDB; 1467963at2759; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 431914; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR006595; CTLH_C.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR006594; LisH.
DR   InterPro; IPR045184; SMU1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22848; PTHR22848; 1.
DR   Pfam; PF00400; WD40; 5.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00668; CTLH; 1.
DR   SMART; SM00667; LisH; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50897; CTLH; 1.
DR   PROSITE; PS50896; LISH; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..513
FT                   /note="WD40 repeat-containing protein SMU1"
FT                   /id="PRO_0000237595"
FT   DOMAIN          6..38
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          40..92
FT                   /note="CTLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT   REPEAT          212..253
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          262..303
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          305..346
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          347..386
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          395..436
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          440..479
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          482..513
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REGION          1..315
FT                   /note="Required for interaction with ik"
FT                   /evidence="ECO:0000250|UniProtKB:Q2TAY7"
SQ   SEQUENCE   513 AA;  57623 MW;  C160DCA8BDD2E29F CRC64;
     MSIEIESSDV IRLIMQYLKE NSLHRTLATL QEETTVSLNT VDSIESFVAD INSGHWDTVL
     QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQNQ SERYIHLENL
     LARSYFDPRE AYPDGSSKEK RRTAIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP
     GMTIDLFRGK AAVKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV
     WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
     RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD
     GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN
     TVVIMNMQGQ IVRSFSSGKR EGGDFVCCTL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL
     TVHEKDVIGI AHHPHQNLIG TYSEDGLLKL WKP