SMXL4_ARATH
ID SMXL4_ARATH Reviewed; 1017 AA.
AC Q9SZR3;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein SMAX1-LIKE 4 {ECO:0000303|PubMed:23893171};
DE Short=AtSMXL4 {ECO:0000303|PubMed:23893171};
DE AltName: Full=Protein HEAT SHOCK PROTEIN-RELATED {ECO:0000303|PubMed:26603028};
DE Short=AtHSPR {ECO:0000303|PubMed:26603028};
DE EC=3.6.3.- {ECO:0000269|PubMed:26603028};
GN Name=SMXL4 {ECO:0000303|PubMed:23893171};
GN Synonyms=HSPR {ECO:0000303|PubMed:26603028};
GN OrderedLocusNames=At4g29920 {ECO:0000312|Araport:AT4G29920};
GN ORFNames=F27B13.160 {ECO:0000312|EMBL:CAB43667.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23893171; DOI=10.1104/pp.113.221259;
RA Stanga J.P., Smith S.M., Briggs W.R., Nelson D.C.;
RT "SUPPRESSOR OF MORE AXILLARY GROWTH2 1 controls seed germination and
RT seedling development in Arabidopsis.";
RL Plant Physiol. 163:318-330(2013).
RN [4]
RP REVIEW.
RX PubMed=25179782; DOI=10.1016/j.pbi.2014.08.001;
RA Bennett T., Leyser O.;
RT "Strigolactone signalling: standing on the shoulders of DWARFs.";
RL Curr. Opin. Plant Biol. 22:7-13(2014).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=24675528; DOI=10.1016/j.plaphy.2014.02.019;
RA Zhang L., Yang T., Li X., Hao H., Xu S., Cheng W., Sun Y., Wang C.;
RT "Cloning and characterization of a novel Athspr promoter specifically
RT active in vascular tissue.";
RL Plant Physiol. Biochem. 78:88-96(2014).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY SLAT AND ABSCISIC ACID.
RX PubMed=26603028; DOI=10.1111/tpj.13080;
RA Yang T., Zhang L., Hao H., Zhang P., Zhu H., Cheng W., Wang Y., Wang X.,
RA Wang C.;
RT "Nuclear-localized AtHSPR links abscisic acid-dependent salt tolerance and
RT antioxidant defense in Arabidopsis.";
RL Plant J. 84:1274-1294(2015).
CC -!- FUNCTION: Probable component of a transcriptional corepressor complex
CC involved in salt tolerance through modulation of reactive oxygen
CC species levels, abscisic acid-dependent stomatal closure,
CC photosynthesis and K(+) /Na(+) homeostasis (PubMed:26603028). Has an in
CC vitro ATPase activity (PubMed:26603028). {ECO:0000269|PubMed:26603028}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=348 uM for ATP {ECO:0000269|PubMed:26603028};
CC Vmax=0.77 umol/h/mg enzyme {ECO:0000269|PubMed:26603028};
CC Note=kcat is 0.17 min(-1) for ATP. {ECO:0000269|PubMed:26603028};
CC -!- SUBUNIT: Interacts probably with TPL/TPR in an EAR-motif dependent
CC manner. {ECO:0000250|UniProtKB:Q9FHH2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26603028}.
CC -!- TISSUE SPECIFICITY: Detected in seedlings and roots (PubMed:23893171).
CC Expressed specifically in vascular tissues (PubMed:24675528). Expressed
CC in flowers, stems, siliques, leaves and roots, with the highest
CC expression in open flowers and the lowest in leaves (PubMed:26603028).
CC {ECO:0000269|PubMed:23893171, ECO:0000269|PubMed:24675528,
CC ECO:0000269|PubMed:26603028}.
CC -!- INDUCTION: Up-regulated by salt and abscisic acid treatment.
CC {ECO:0000269|PubMed:26603028}.
CC -!- DISRUPTION PHENOTYPE: Reduced drought tolerance and hypersensitivity to
CC salt stress, resulting in poor growth in normal soil.
CC {ECO:0000269|PubMed:26603028}.
CC -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR EMBL; AL050352; CAB43667.1; -; Genomic_DNA.
DR EMBL; AL161575; CAB79750.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85693.1; -; Genomic_DNA.
DR PIR; T08553; T08553.
DR RefSeq; NP_194721.1; NM_119138.2.
DR AlphaFoldDB; Q9SZR3; -.
DR STRING; 3702.AT4G29920.1; -.
DR iPTMnet; Q9SZR3; -.
DR PaxDb; Q9SZR3; -.
DR PRIDE; Q9SZR3; -.
DR EnsemblPlants; AT4G29920.1; AT4G29920.1; AT4G29920.
DR GeneID; 829115; -.
DR Gramene; AT4G29920.1; AT4G29920.1; AT4G29920.
DR KEGG; ath:AT4G29920; -.
DR Araport; AT4G29920; -.
DR TAIR; locus:2123944; AT4G29920.
DR eggNOG; KOG1051; Eukaryota.
DR HOGENOM; CLU_006575_0_0_1; -.
DR InParanoid; Q9SZR3; -.
DR PhylomeDB; Q9SZR3; -.
DR SABIO-RK; Q9SZR3; -.
DR PRO; PR:Q9SZR3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZR3; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0033500; P:carbohydrate homeostasis; IGI:TAIR.
DR GO; GO:0042631; P:cellular response to water deprivation; IMP:TAIR.
DR GO; GO:0010233; P:phloem transport; IGI:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR Gene3D; 1.10.1780.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR036628; Clp_N_dom_sf.
DR InterPro; IPR004176; Clp_R_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02861; Clp_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF81923; SSF81923; 1.
DR PROSITE; PS51903; CLP_R; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..1017
FT /note="Protein SMAX1-LIKE 4"
FT /id="PRO_0000435713"
FT DOMAIN 8..183
FT /note="Clp R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 12..91
FT /note="Repeat 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 105..183
FT /note="Repeat 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 196..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 859..863
FT /note="EAR"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1017 AA; 113784 MW; 90809F6CBCB99821 CRC64;
MRTGAYTVHQ TLTPEAASVL KQSLTLARRR GHSQVTPLHV ASTLLTSSRS NLFRRACLKS
NPFTALGRQM AHPSLHCRAL ELCFNVSLNR LPTNPNPLFQ TQPSLSNALV AALKRAQAHQ
RRGCVEQQQS QQNQPFLAVK VELEQLVVSI LDDPSVSRVM REAGLSSVSV KSNIEDDSSV
VSPVFYGSSS SVGVFSSPCS PSSSENNQGG GTLSPNPSKI WHAHLTNHHS FEQNPFFHFP
KGKTFTPDQA FPVREDANPV IEVLLGKKNN KKRNTVIVGD SVSLTEGVVA KLMGRIERGE
VPDDLKQTHF IKFQFSQVGL NFMKKEDIEG QVRELKRKID SFTSWGGKGV IVCLGDLDWA
VWGGGNSASS SNYSAADHLV EEIGRLVYDY SNTGAKVWLL GTASYQTYMR CQMKQPPLDV
HWALQAVSIP SGGLSLTLHA SSSEMASQVM EMKPFRVKEE EEGAREEEEE DKLNFCGECA
FNYEKEAKAF ISAQHKILPP WLQPHGDNNN INQKDELSGL RKKWNRFCQA LHHKKPSMTA
WRAEQSSSVL PGSLMDSSLK QNSRASSSVA KFRRQNSCTI EFSFGSNRQE GLKKTDELSL
DGFKSNNDEG VKTKITLALG HSPFPSDSEN SEEEEPEKAI KMSKLLEKLH ENIPWQKDVL
PSIVEAMEES VKRSKRKDAW MLVSGNDVTA KRRLAITLTT SLFGSHENML KINLRTSKAS
EACEELKNAL KKKEEVVILI ERVDLADAQF MNILVDRFEA GDLDGFQGKK SQIIFLLTRE
DDECVENEHF VIPMVLNCNK SGSGLVNNKR KPEYDAAPTM IKKKNPRIEE DDDESNVACD
ISNIKKEFSR QLKFESNALD LNLRVDADED EEEEAKPATE ISSGFEERFL DSIQNRFDFT
VLSDEDITKF FVTKIKDSCE EILGQREERF GFTVDAELIE KFYKGCGFFA NGLFEEWVKE
VFQRGLVTVK NGGKEGISVI NLCLGGIDMI DQGEVYEEEE GFMGTCLPNR IHVSFVD
