ID   SMXL7_ARATH             Reviewed;        1002 AA.
AC   O80875; Q94BS5;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Protein SMAX1-LIKE 7 {ECO:0000303|PubMed:23893171};
DE            Short=AtSMXL7 {ECO:0000303|PubMed:23893171};
DE   AltName: Full=Protein D53-like 1 {ECO:0000303|PubMed:24336200};
DE            Short=AtD53-like 1 {ECO:0000303|PubMed:24336200};
DE   AltName: Full=Protein D53-like SMXL 7 {ECO:0000303|PubMed:26546446};
GN   Name=SMXL7 {ECO:0000303|PubMed:23893171};
GN   OrderedLocusNames=At2g29970 {ECO:0000312|Araport:AT2G29970};
GN   ORFNames=F23F1.11 {ECO:0000312|EMBL:AAC31855.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=24336200; DOI=10.1038/nature12870;
RA   Jiang L., Liu X., Xiong G., Liu H., Chen F., Wang L., Meng X., Liu G.,
RA   Yu H., Yuan Y., Yi W., Zhao L., Ma H., He Y., Wu Z., Melcher K., Qian Q.,
RA   Xu H.E., Wang Y., Li J.;
RT   "DWARF 53 acts as a repressor of strigolactone signalling in rice.";
RL   Nature 504:401-405(2013).
RN   [5]
RP   TISSUE SPECIFICITY, INDUCTION BY STRIGOLACTONE, GENE FAMILY, AND
RP   NOMENCLATURE.
RX   PubMed=23893171; DOI=10.1104/pp.113.221259;
RA   Stanga J.P., Smith S.M., Briggs W.R., Nelson D.C.;
RT   "SUPPRESSOR OF MORE AXILLARY GROWTH2 1 controls seed germination and
RT   seedling development in Arabidopsis.";
RL   Plant Physiol. 163:318-330(2013).
RN   [6]
RP   REVIEW.
RX   PubMed=25179782; DOI=10.1016/j.pbi.2014.08.001;
RA   Bennett T., Leyser O.;
RT   "Strigolactone signalling: standing on the shoulders of DWARFs.";
RL   Curr. Opin. Plant Biol. 22:7-13(2014).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MAX2; TPR2 AND D14,
RP   MUTAGENESIS OF 854-LEU--PRO-859, AND UBIQUITINATION.
RX   PubMed=26546446; DOI=10.1105/tpc.15.00605;
RA   Wang L., Wang B., Jiang L., Liu X., Li X., Lu Z., Meng X., Wang Y.,
RA   Smith S.M., Li J.;
RT   "Strigolactone signaling in Arabidopsis regulates shoot development by
RT   targeting D53-like SMXL repressor proteins for ubiquitination and
RT   degradation.";
RL   Plant Cell 27:3128-3142(2015).
RN   [8]
RP   FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 718-PHE--ASP-725, TISSUE
RP   SPECIFICITY, INTERACTION WITH TPL; TPR1; TPR2 AND TPR4, AND EAR MOTIF.
RX   PubMed=26546447; DOI=10.1105/tpc.15.00562;
RA   Soundappan I., Bennett T., Morffy N., Liang Y., Stanga J.P., Abbas A.,
RA   Leyser O., Nelson D.C.;
RT   "SMAX1-LIKE/D53 family members enable distinct MAX2-dependent responses to
RT   strigolactones and karrikins in Arabidopsis.";
RL   Plant Cell 27:3143-3159(2015).
RN   [9]
RP   INTERACTION WITH D14.
RX   PubMed=25713176; DOI=10.1093/pcp/pcv028;
RA   Umehara M., Cao M., Akiyama K., Akatsu T., Seto Y., Hanada A., Li W.,
RA   Takeda-Kamiya N., Morimoto Y., Yamaguchi S.;
RT   "Structural requirements of strigolactones for shoot branching inhibition
RT   in rice and Arabidopsis.";
RL   Plant Cell Physiol. 56:1059-1072(2015).
CC   -!- FUNCTION: Probable component of a transcriptional corepressor complex
CC       involved in branching control. Regulates cotyledon expansion and
CC       lateral root growth, but not germination or hypocotyl elongation.
CC       Promotes auxin transport and PIN1 accumulation in the stem and
CC       represses BRC1/TCP18 expression in axillary buds (PubMed:26546447,
CC       PubMed:26546446). {ECO:0000269|PubMed:26546446,
CC       ECO:0000269|PubMed:26546447}.
CC   -!- SUBUNIT: Interacts with TPL/TPR in an EAR-motif dependent manner
CC       (PubMed:26546447). Interacts with TPL, TPR1, TPR2 and TPR4
CC       (PubMed:26546447). Interacts with MAX2 and TPR2 (PubMed:26546446).
CC       Interacts with D14 (PubMed:26546446, PubMed:25713176). The interaction
CC       with D14 occurs in the presence of (2'R) stereoisomers of
CC       strigolactones, but not (2'S) stereoisomers (PubMed:25713176).
CC       {ECO:0000269|PubMed:25713176, ECO:0000269|PubMed:26546446,
CC       ECO:0000269|PubMed:26546447}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26546446}.
CC   -!- TISSUE SPECIFICITY: Expressed in axillary branches and roots. Detected
CC       in seedlings and leaves (PubMed:23893171). Expressed in the primary
CC       rosette buds and expanding leaves of adult rosettes, the vasculature of
CC       the hypocotyls, cotyledons, and mature roots, and in the midvein and
CC       petioles of young leaves (PubMed:26546447).
CC       {ECO:0000269|PubMed:23893171, ECO:0000269|PubMed:26546447}.
CC   -!- INDUCTION: Up-regulated by strigolactone treatment.
CC       {ECO:0000269|PubMed:23893171}.
CC   -!- DOMAIN: Contains 1 EAR motif required for the interaction with TPR2.
CC       {ECO:0000269|PubMed:26546446, ECO:0000269|PubMed:26546447}.
CC   -!- PTM: Ubiquitinated upon strigolactone treatment. Strigolactone, but not
CC       karrikin, triggers rapid SCF(MAX2)-dependent degradation
CC       (PubMed:26546447, PubMed:26546446). {ECO:0000269|PubMed:26546446,
CC       ECO:0000269|PubMed:26546447}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype. Suppresses max2 phenotypes
CC       associated with strigolactone-D14-regulated growth. Smxl7 and max2
CC       double mutants have reduced branching and increased inflorescence
CC       heights compared with max2 mutants. {ECO:0000269|PubMed:26546447}.
CC   -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR   EMBL; AC004680; AAC31855.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08329.1; -; Genomic_DNA.
DR   EMBL; AY039922; AAK64026.1; -; mRNA.
DR   PIR; T02488; T02488.
DR   RefSeq; NP_565689.1; NM_128551.4.
DR   AlphaFoldDB; O80875; -.
DR   STRING; 3702.AT2G29970.1; -.
DR   iPTMnet; O80875; -.
DR   PaxDb; O80875; -.
DR   PRIDE; O80875; -.
DR   ProteomicsDB; 232477; -.
DR   EnsemblPlants; AT2G29970.1; AT2G29970.1; AT2G29970.
DR   GeneID; 817547; -.
DR   Gramene; AT2G29970.1; AT2G29970.1; AT2G29970.
DR   KEGG; ath:AT2G29970; -.
DR   Araport; AT2G29970; -.
DR   TAIR; locus:2045653; AT2G29970.
DR   eggNOG; KOG1051; Eukaryota.
DR   HOGENOM; CLU_006575_0_2_1; -.
DR   InParanoid; O80875; -.
DR   OMA; RTRCPPI; -.
DR   OrthoDB; 182446at2759; -.
DR   PhylomeDB; O80875; -.
DR   PRO; PR:O80875; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O80875; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.1780.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036628; Clp_N_dom_sf.
DR   InterPro; IPR004176; Clp_R_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51903; CLP_R; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Ubl conjugation.
FT   CHAIN           1..1002
FT                   /note="Protein SMAX1-LIKE 7"
FT                   /id="PRO_0000435716"
FT   DOMAIN          8..185
FT                   /note="Clp R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          12..86
FT                   /note="Repeat 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          103..185
FT                   /note="Repeat 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   MOTIF           854..858
FT                   /note="EAR"
FT                   /evidence="ECO:0000305|PubMed:26546447"
FT   MUTAGEN         718..725
FT                   /note="Missing: Resistant to degradation after
FT                   strigolactone treatment."
FT                   /evidence="ECO:0000269|PubMed:26546447"
FT   MUTAGEN         854..859
FT                   /note="Missing: Loss of interaction with TPR2."
FT                   /evidence="ECO:0000269|PubMed:26546446"
FT   CONFLICT        736
FT                   /note="D -> G (in Ref. 3; AAK64026)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1002 AA;  111924 MW;  225EC3EF7C8EC438 CRC64;
     MPTPVTTARQ CLTEETARAL DDAVSVARRR SHAQTTSLHA VSGLLTMPSS ILREVCISRA
     AHNTPYSSRL QFRALELCVG VSLDRLPSSK STPTTTVEED PPVSNSLMAA IKRSQATQRR
     HPETYHLHQI HGNNNTETTS VLKVELKYFI LSILDDPIVS RVFGEAGFRS TDIKLDVLHP
     PVTSQFSSRF TSRSRIPPLF LCNLPESDSG RVRFGFPFGD LDENCRRIGE VLARKDKKNP
     LLVGVCGVEA LKTFTDSINR GKFGFLPLEI SGLSVVSIKI SEVLVDGSRI DIKFDDLGRL
     KSGMVLNLGE LKVLASDVFS VDVIEKFVLK LADLLKLHRE KLWFIGSVSS NETYLKLIER
     FPTIDKDWNL HLLPITSSSQ GLYPKSSLMG SFVPFGGFFS STSDFRIPSS SSMNQTLPRC
     HLCNEKYEQE VTAFAKSGSM IDDQCSEKLP SWLRNVEHEH EKGNLGKVKD DPNVLASRIP
     ALQKKWDDIC QRIHQTPAFP KLSFQPVRPQ FPLQLGSSSQ TKMSLGSPTE KIVCTRTSES
     FQGMVALPQN PPHQPGLSVK ISKPKHTEDL SSSTTNSPLS FVTTDLGLGT IYASKNQEPS
     TPVSVERRDF EVIKEKQLLS ASRYCKDFKS LRELLSRKVG FQNEAVNAIS EIVCGYRDES
     RRRNNHVATT SNVWLALLGP DKAGKKKVAL ALAEVFCGGQ DNFICVDFKS QDSLDDRFRG
     KTVVDYIAGE VARRADSVVF IENVEKAEFP DQIRLSEAMR TGKLRDSHGR EISMKNVIVV
     ATISGSDKAS DCHVLEEPVK YSEERVLNAK NWTLQIKLAD TSNVNKNGPN KRRQEEAETE
     VTELRALKSQ RSFLDLNLPV DEIEANEDEA YTMSENTEAW LEDFVEQVDG KVTFKLIDFD
     ELAKNIKRNI LSLFHLSFGP ETHLEIENDV ILKILAALRW SSDEEKTFDQ WLQTVLAPSF
     AKARQKCVPA APFSVKLVAS RESPAEEETT GIQQFPARVE VI