SMXL8_ARATH
ID SMXL8_ARATH Reviewed; 910 AA.
AC F4IGZ2; Q940S4; Q9XEE5;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Protein SMAX1-LIKE 8 {ECO:0000303|PubMed:23893171};
DE Short=AtSMXL8 {ECO:0000303|PubMed:23893171};
DE AltName: Full=Protein D53-like 3 {ECO:0000303|PubMed:24336200};
DE Short=AtD53-like 3 {ECO:0000303|PubMed:24336200};
DE AltName: Full=Protein D53-like SMXL 8 {ECO:0000303|PubMed:26546446};
GN Name=SMXL8 {ECO:0000303|PubMed:23893171};
GN OrderedLocusNames=At2g40130 {ECO:0000312|Araport:AT2G40130};
GN ORFNames=T07M07.2 {ECO:0000312|EMBL:AAD25929.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10207155;
RA Wang M.L., Belmonte S., Kim U., Dolan M., Morris J.W., Goodman H.M.;
RT "A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07.";
RL Genome Res. 9:325-333(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION.
RX PubMed=24336200; DOI=10.1038/nature12870;
RA Jiang L., Liu X., Xiong G., Liu H., Chen F., Wang L., Meng X., Liu G.,
RA Yu H., Yuan Y., Yi W., Zhao L., Ma H., He Y., Wu Z., Melcher K., Qian Q.,
RA Xu H.E., Wang Y., Li J.;
RT "DWARF 53 acts as a repressor of strigolactone signalling in rice.";
RL Nature 504:401-405(2013).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION BY KARRIKINS AND STRIGOLACTONE, GENE FAMILY,
RP AND NOMENCLATURE.
RX PubMed=23893171; DOI=10.1104/pp.113.221259;
RA Stanga J.P., Smith S.M., Briggs W.R., Nelson D.C.;
RT "SUPPRESSOR OF MORE AXILLARY GROWTH2 1 controls seed germination and
RT seedling development in Arabidopsis.";
RL Plant Physiol. 163:318-330(2013).
RN [7]
RP REVIEW.
RX PubMed=25179782; DOI=10.1016/j.pbi.2014.08.001;
RA Bennett T., Leyser O.;
RT "Strigolactone signalling: standing on the shoulders of DWARFs.";
RL Curr. Opin. Plant Biol. 22:7-13(2014).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MAX2; TPR2 AND D14,
RP MUTAGENESIS OF 768-LEU--PRO-773, AND UBIQUITINATION.
RX PubMed=26546446; DOI=10.1105/tpc.15.00605;
RA Wang L., Wang B., Jiang L., Liu X., Li X., Lu Z., Meng X., Wang Y.,
RA Smith S.M., Li J.;
RT "Strigolactone signaling in Arabidopsis regulates shoot development by
RT targeting D53-like SMXL repressor proteins for ubiquitination and
RT degradation.";
RL Plant Cell 27:3128-3142(2015).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=26546447; DOI=10.1105/tpc.15.00562;
RA Soundappan I., Bennett T., Morffy N., Liang Y., Stanga J.P., Abbas A.,
RA Leyser O., Nelson D.C.;
RT "SMAX1-LIKE/D53 family members enable distinct MAX2-dependent responses to
RT strigolactones and karrikins in Arabidopsis.";
RL Plant Cell 27:3143-3159(2015).
RN [10]
RP INTERACTION WITH D14.
RX PubMed=25713176; DOI=10.1093/pcp/pcv028;
RA Umehara M., Cao M., Akiyama K., Akatsu T., Seto Y., Hanada A., Li W.,
RA Takeda-Kamiya N., Morimoto Y., Yamaguchi S.;
RT "Structural requirements of strigolactones for shoot branching inhibition
RT in rice and Arabidopsis.";
RL Plant Cell Physiol. 56:1059-1072(2015).
CC -!- FUNCTION: Probable component of a transcriptional corepressor complex
CC involved in branching control. Regulates cotyledon expansion and
CC lateral root growth, but not germination or hypocotyl elongation.
CC Promotes auxin transport and PIN1 accumulation in the stem and
CC represses BRC1/TCP18 expression in axillary buds (PubMed:26546447,
CC PubMed:26546446). {ECO:0000269|PubMed:26546446,
CC ECO:0000269|PubMed:26546447}.
CC -!- SUBUNIT: Interacts with TPL/TPR in an EAR-motif dependent manner.
CC Interacts with MAX2 and TPR2 (PubMed:26546446). Interacts with D14
CC (PubMed:26546446, PubMed:25713176). The interaction with D14 occurs in
CC the presence of (2'R) stereoisomers of strigolactones, but not (2'S)
CC stereoisomers (PubMed:25713176). {ECO:0000269|PubMed:25713176,
CC ECO:0000269|PubMed:26546446}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26546446}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IGZ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IGZ2-2; Sequence=VSP_058154;
CC -!- TISSUE SPECIFICITY: Detected inaxillary branches and leaves
CC (PubMed:23893171). Expressed in the primary rosette buds and expanding
CC leaves of adult rosettes, the vasculature of the hypocotyls,
CC cotyledons, and mature roots, and in the midvein and petioles of young
CC leaves (PubMed:26546447). {ECO:0000269|PubMed:23893171,
CC ECO:0000269|PubMed:26546447}.
CC -!- INDUCTION: Up-regulated by karrikins and strigolactone treatments.
CC {ECO:0000269|PubMed:23893171}.
CC -!- DOMAIN: Contains 1 EAR motif required for the interaction with TPR2.
CC {ECO:0000269|PubMed:26546446}.
CC -!- PTM: Ubiquitinated upon strigolactone treatment (PubMed:26546446).
CC Probable proteolytic target of SCF(MAX2)-mediated stigolactone
CC signaling (PubMed:26546447). {ECO:0000269|PubMed:26546446,
CC ECO:0000269|PubMed:26546447}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Suppresses max2 phenotypes
CC associated with strigolactone-D14-regulated growth. Smxl8 and max2
CC double mutants have branching and inflorescence heights similar to max2
CC mutants. {ECO:0000269|PubMed:26546447}.
CC -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25929.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF085279; AAD25929.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09786.1; -; Genomic_DNA.
DR EMBL; AY053407; AAK96637.1; -; mRNA.
DR EMBL; AY093959; AAM16220.1; -; mRNA.
DR RefSeq; NP_973646.1; NM_201917.1. [F4IGZ2-1]
DR AlphaFoldDB; F4IGZ2; -.
DR STRING; 3702.AT2G40130.2; -.
DR iPTMnet; F4IGZ2; -.
DR PaxDb; F4IGZ2; -.
DR PRIDE; F4IGZ2; -.
DR EnsemblPlants; AT2G40130.2; AT2G40130.2; AT2G40130. [F4IGZ2-1]
DR GeneID; 818604; -.
DR Gramene; AT2G40130.2; AT2G40130.2; AT2G40130. [F4IGZ2-1]
DR KEGG; ath:AT2G40130; -.
DR Araport; AT2G40130; -.
DR TAIR; locus:2065074; AT2G40130.
DR eggNOG; KOG1051; Eukaryota.
DR HOGENOM; CLU_006575_0_2_1; -.
DR InParanoid; F4IGZ2; -.
DR OMA; RRDIWFN; -.
DR OrthoDB; 182446at2759; -.
DR PRO; PR:F4IGZ2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IGZ2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:1902347; P:response to strigolactone; IMP:TAIR.
DR Gene3D; 1.10.1780.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR036628; Clp_N_dom_sf.
DR InterPro; IPR004176; Clp_R_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF07724; AAA_2; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF81923; SSF81923; 1.
DR PROSITE; PS51903; CLP_R; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..910
FT /note="Protein SMAX1-LIKE 8"
FT /id="PRO_0000435717"
FT DOMAIN 7..179
FT /note="Clp R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 12..84
FT /note="Repeat 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 98..179
FT /note="Repeat 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 504..524
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 768..772
FT /note="EAR"
FT /evidence="ECO:0000305|PubMed:26546446"
FT VAR_SEQ 460..910
FT /note="VVQTKEGLESVCGNKFTSSASASTCSAKSVTTDLNLRVSSVTTGSGLKKHLD
FT SKDFSQPQSVSSYSFDNPRDLNAESFKIIYRRLTDMVSGQDEAARVISCALSQPPKSVT
FT RRDVWLNLVGPDTVGKRRMSLVLAEIVYQSEHRFMAVDLGAAEQGMGGCDDPMRLRGKT
FT MVDHIFEVMCRNPFCVVFLENIEKADEKLQMSLSKAIETGKFMDSHGREVGIGNTIFVM
FT TSSSQGSATTTSYSEEKLLRVKGRQVEIRIETVSSLPMVRSVYGPTSVNKRKLMGLGNL
FT QETKDTVESVKRLNRTTNGVLDLNLPAQETEIEEKYHCEENSNVWLMNLKNHKRLIEVP
FT FKPFDFEGLAEKIKKSVKENFDKCVRSDCLLEVDPKIIERLLAAVYFSDSRKDIKELLE
FT NIMSPVFLRIKERYEITTSCVVKLVGRDLDIFLEDQMDLFFVKSQ -> CRPKKGWNQF
FT VEISSRAVLLHRPVQLNRSPLI (in isoform 2)"
FT /id="VSP_058154"
FT MUTAGEN 768..773
FT /note="Missing: Loss of interaction with TPR2."
FT /evidence="ECO:0000269|PubMed:26546446"
SQ SEQUENCE 910 AA; 101611 MW; 972473C91590CEA6 CRC64;
MPTAVNVAKQ CLTAEASYAL EEAVNVARRR GHSQTTSLHA ISALLSLPTS VLRDACARVR
NSAYSPRLQF KALDLCLSVS LDRIQSGHQL GSDDSPPVSN SLMAAIKRSQ AHQRRLPENF
RIYQEMSQSQ NQNSLSCVKV ELRQLILSIL DDPVVSRVFG EAGFRSSELK LSIIRPVPHL
LRYSSQQPLF LCNLTGNPEP NPVRWGFTVP SLNFNGDLDY RRISAVFTKD KGRNPLLVGV
SAYGVLTSYL NSLEKNQTDG MILPTKLHGL TAVNIGSEIS DQISVKFDKT YTDTRFHDLG
KLAEQGSGPG LLLHYGDLRV FTNGEGNVPA ANYIVNRISE LLRRHGRRVW LIGATTSNEV
YEKMMRRFPN VEKDWDLQLL TITSLKPCLP HNKSSLIGSF VPFGGFFSTT PSELKLPFSG
FKTEITGPVS SISDQTQSTL PPWLQMTTRT DLNQKSSAKV VQTKEGLESV CGNKFTSSAS
ASTCSAKSVT TDLNLRVSSV TTGSGLKKHL DSKDFSQPQS VSSYSFDNPR DLNAESFKII
YRRLTDMVSG QDEAARVISC ALSQPPKSVT RRDVWLNLVG PDTVGKRRMS LVLAEIVYQS
EHRFMAVDLG AAEQGMGGCD DPMRLRGKTM VDHIFEVMCR NPFCVVFLEN IEKADEKLQM
SLSKAIETGK FMDSHGREVG IGNTIFVMTS SSQGSATTTS YSEEKLLRVK GRQVEIRIET
VSSLPMVRSV YGPTSVNKRK LMGLGNLQET KDTVESVKRL NRTTNGVLDL NLPAQETEIE
EKYHCEENSN VWLMNLKNHK RLIEVPFKPF DFEGLAEKIK KSVKENFDKC VRSDCLLEVD
PKIIERLLAA VYFSDSRKDI KELLENIMSP VFLRIKERYE ITTSCVVKLV GRDLDIFLED
QMDLFFVKSQ
