SN25A_DANRE
ID SN25A_DANRE Reviewed; 204 AA.
AC Q5TZ66; O93578; Q5TZ65; Q6P3L7;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Synaptosomal-associated protein 25-A {ECO:0000250|UniProtKB:P36977};
DE Short=SNAP-25A {ECO:0000250|UniProtKB:P36977};
DE AltName: Full=Synaptosome-associated protein 25.1 {ECO:0000303|PubMed:9843147};
DE Short=SNAP-25.1 {ECO:0000303|PubMed:9843147};
GN Name=snap25a {ECO:0000312|ZFIN:ZDB-GENE-980526-468};
GN Synonyms=Snap {ECO:0000312|EMBL:AAC64289.1},
GN snap25.1 {ECO:0000303|PubMed:9843147}; ORFNames=si:dkeyp-8f4.6;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAH69031.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAC64289.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 81-204 (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=9843147;
RX DOI=10.1002/(sici)1097-4547(19981201)54:5<563::aid-jnr1>3.0.co;2-7;
RA Risinger C., Salaneck E., Soederberg C., Gates M., Postlethwait J.H.,
RA Larhammar D.;
RT "Cloning of two loci for synapse protein Snap25 in zebrafish: comparison of
RT paralogous linkage groups suggests loss of one locus in the mammalian
RT lineage.";
RL J. Neurosci. Res. 54:563-573(1998).
CC -!- FUNCTION: May play an important role in the synaptic function of
CC specific neuronal systems. Associates with proteins involved in vesicle
CC docking and membrane fusion. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Synapse, synaptosome
CC {ECO:0000250|UniProtKB:P36977, ECO:0000250|UniProtKB:P60880}. Cell
CC membrane {ECO:0000250|UniProtKB:P60881}. Note=Complexed with
CC macromolecular elements of the nerve terminal.
CC {ECO:0000250|UniProtKB:P36977, ECO:0000250|UniProtKB:P60880}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Isoforms differ by the usage of two alternative homologous
CC exons which code for positions 55 to 94 and differ only in 11
CC positions out of 40.;
CC Name=1 {ECO:0000269|PubMed:9843147};
CC IsoId=Q5TZ66-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5TZ66-2; Sequence=VSP_052974;
CC -!- TISSUE SPECIFICITY: Expressed in several regions throughout the adult
CC brain, including the mesencephalon. {ECO:0000269|PubMed:9843147}.
CC -!- SIMILARITY: Belongs to the SNAP-25 family. {ECO:0000255}.
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DR EMBL; BX465184; CAH69031.1; -; Genomic_DNA.
DR EMBL; BX470246; CAH69031.1; JOINED; Genomic_DNA.
DR EMBL; BX465184; CAH69032.1; -; Genomic_DNA.
DR EMBL; BX470246; CAH69032.1; JOINED; Genomic_DNA.
DR EMBL; BX470246; CAI21359.1; -; Genomic_DNA.
DR EMBL; BX465184; CAI21359.1; JOINED; Genomic_DNA.
DR EMBL; BX470246; CAI21360.1; -; Genomic_DNA.
DR EMBL; BX465184; CAI21360.1; JOINED; Genomic_DNA.
DR EMBL; BC063942; AAH63942.1; -; mRNA.
DR EMBL; AF091593; AAC64289.1; -; mRNA.
DR RefSeq; NP_571510.1; NM_131435.1.
DR RefSeq; XP_005160680.1; XM_005160623.3. [Q5TZ66-2]
DR AlphaFoldDB; Q5TZ66; -.
DR SMR; Q5TZ66; -.
DR STRING; 7955.ENSDARP00000019054; -.
DR PaxDb; Q5TZ66; -.
DR Ensembl; ENSDART00000007584; ENSDARP00000019054; ENSDARG00000020609. [Q5TZ66-1]
DR Ensembl; ENSDART00000062738; ENSDARP00000062737; ENSDARG00000020609. [Q5TZ66-2]
DR GeneID; 30712; -.
DR KEGG; dre:30712; -.
DR CTD; 30712; -.
DR ZFIN; ZDB-GENE-980526-468; snap25a.
DR eggNOG; KOG3065; Eukaryota.
DR GeneTree; ENSGT00950000182843; -.
DR HOGENOM; CLU_096939_0_0_1; -.
DR InParanoid; Q5TZ66; -.
DR OMA; WKASEDG; -.
DR OrthoDB; 1197028at2759; -.
DR PhylomeDB; Q5TZ66; -.
DR TreeFam; TF315125; -.
DR Reactome; R-DRE-181429; Serotonin Neurotransmitter Release Cycle.
DR Reactome; R-DRE-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-DRE-210500; Glutamate Neurotransmitter Release Cycle.
DR Reactome; R-DRE-212676; Dopamine Neurotransmitter Release Cycle.
DR Reactome; R-DRE-264642; Acetylcholine Neurotransmitter Release Cycle.
DR Reactome; R-DRE-449836; Other interleukin signaling.
DR Reactome; R-DRE-6798695; Neutrophil degranulation.
DR Reactome; R-DRE-888590; GABA synthesis, release, reuptake and degradation.
DR PRO; PR:Q5TZ66; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000020609; Expressed in brain and 31 other tissues.
DR ExpressionAtlas; Q5TZ66; baseline and differential.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:ZFIN.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0031201; C:SNARE complex; ISS:UniProtKB.
DR GO; GO:0070032; C:synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex; IBA:GO_Central.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0017075; F:syntaxin-1 binding; IBA:GO_Central.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0007626; P:locomotory behavior; IMP:ZFIN.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:ZFIN.
DR GO; GO:0031629; P:synaptic vesicle fusion to presynaptic active zone membrane; IBA:GO_Central.
DR GO; GO:0016082; P:synaptic vesicle priming; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR InterPro; IPR039077; SNAP-25.
DR InterPro; IPR000928; SNAP-25_dom.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19305:SF5; PTHR19305:SF5; 1.
DR Pfam; PF00835; SNAP-25; 1.
DR SMART; SM00397; t_SNARE; 2.
DR PROSITE; PS50192; T_SNARE; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Repeat; Synapse; Synaptosome.
FT CHAIN 1..204
FT /note="Synaptosomal-associated protein 25-A"
FT /id="PRO_0000355574"
FT DOMAIN 19..81
FT /note="t-SNARE coiled-coil homology 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT DOMAIN 138..200
FT /note="t-SNARE coiled-coil homology 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 58..89
FT /note="ERIEEGMDQINKDMKDAEKNLNDLGKFCGLCS -> DRVEDGMNHINQDMKE
FT AEKNLKDLGKCCGLFI (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_052974"
FT CONFLICT 190
FT /note="I -> F (in Ref. 2; AAH63942)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 204 AA; 22857 MW; B53CF5F3D1C69EA5 CRC64;
MAEDSDMRNE LADMQQRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI
EEGMDQINKD MKDAEKNLND LGKFCGLCSC PCNKMKSGAS KAWGNNQDGV VASQPARVVD
EREQMAISGG FIRRVTDDAR ENEMDENLEQ VGGIIGNLRH MALDMGNEID TQNRQIDRIM
EKADSNKTRI DEANQRATKM LGSG
