SNA3_YEAST
ID SNA3_YEAST Reviewed; 133 AA.
AC P14359; D6VW35; Q6B236;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 3.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Protein SNA3;
GN Name=SNA3; OrderedLocusNames=YJL151C; ORFNames=J0630;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2642902; DOI=10.1016/s0021-9258(19)85082-2;
RA Dean-Johnson M., Henry S.A.;
RT "Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-
RT phosphate synthase (EC 5.5.1.4) and functional analysis of its structural
RT gene, the INO1 locus.";
RL J. Biol. Chem. 264:1274-1283(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8813765;
RX DOI=10.1002/(sici)1097-0061(19960630)12:8<787::aid-yea954>3.0.co;2-4;
RA Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.;
RT "Sequence analysis of a 40.7 kb segment from the left arm of yeast
RT chromosome X reveals 14 known genes and 13 new open reading frames
RT including homologues of genes clustered on the right arm of chromosome
RT XI.";
RL Yeast 12:787-797(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=11566881; DOI=10.1093/emboj/20.18.5176;
RA Reggiori F., Pelham H.R.B.;
RT "Sorting of proteins into multivesicular bodies: ubiquitin-dependent and
RT -independent targeting.";
RL EMBO J. 20:5176-5186(2001).
RN [7]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=SUB592;
RX PubMed=12872131; DOI=10.1038/nbt849;
RA Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G.,
RA Roelofs J., Finley D., Gygi S.P.;
RT "A proteomics approach to understanding protein ubiquitination.";
RL Nat. Biotechnol. 21:921-926(2003).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=14557538; DOI=10.1073/pnas.2135500100;
RA Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.;
RT "A subset of membrane-associated proteins is ubiquitinated in response to
RT mutations in the endoplasmic reticulum degradation machinery.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003).
RN [11]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [12]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- INTERACTION:
CC P14359; P40540: EMC5; NbExp=3; IntAct=EBI-26122, EBI-24977;
CC P14359; P39940: RSP5; NbExp=2; IntAct=EBI-26122, EBI-16219;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Late
CC endosome membrane; Multi-pass membrane protein. Vacuole lumen.
CC Cytoplasmic vesicle membrane {ECO:0000305}; Multi-pass membrane protein
CC {ECO:0000305}. Note=Sorted via late endosomes to the vacuolar lumen in
CC a ubiquitin-independent manner.
CC -!- MISCELLANEOUS: Present with 12500 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the UPF0057 (PMP3) family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-13 is the initiator.
CC {ECO:0000305}.
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DR EMBL; J04453; AAA66309.1; -; Genomic_DNA.
DR EMBL; Z49427; CAA89447.1; -; Genomic_DNA.
DR EMBL; X87371; CAA60804.1; -; Genomic_DNA.
DR EMBL; AY692894; AAT92913.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08651.1; -; Genomic_DNA.
DR PIR; A32209; B30902.
DR RefSeq; NP_012384.1; NM_001181584.1.
DR PDB; 4LCD; X-ray; 3.10 A; C/D=104-127.
DR PDBsum; 4LCD; -.
DR AlphaFoldDB; P14359; -.
DR SMR; P14359; -.
DR BioGRID; 33608; 111.
DR DIP; DIP-1730N; -.
DR IntAct; P14359; 57.
DR MINT; P14359; -.
DR STRING; 4932.YJL151C; -.
DR TCDB; 9.B.12.3.1; the sensitivity to sodium or salt stress-induced hydrophobic peptide (sna) family.
DR iPTMnet; P14359; -.
DR MaxQB; P14359; -.
DR PaxDb; P14359; -.
DR PRIDE; P14359; -.
DR TopDownProteomics; P14359; -.
DR EnsemblFungi; YJL151C_mRNA; YJL151C; YJL151C.
DR GeneID; 853290; -.
DR KEGG; sce:YJL151C; -.
DR SGD; S000003687; SNA3.
DR VEuPathDB; FungiDB:YJL151C; -.
DR eggNOG; KOG1773; Eukaryota.
DR GeneTree; ENSGT00940000176576; -.
DR HOGENOM; CLU_107649_0_1_1; -.
DR InParanoid; P14359; -.
DR OMA; HATAPAX; -.
DR BioCyc; YEAST:G3O-31594-MON; -.
DR ChiTaRS; SNA3; yeast.
DR PRO; PR:P14359; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P14359; protein.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR GO; GO:0000328; C:fungal-type vacuole lumen; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:SGD.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IDA:SGD.
DR GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; IMP:SGD.
DR GO; GO:0030163; P:protein catabolic process; IMP:SGD.
DR InterPro; IPR000612; PMP3.
DR PANTHER; PTHR21659; PTHR21659; 1.
DR Pfam; PF01679; Pmp3; 1.
DR PROSITE; PS01309; UPF0057; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasmic vesicle; Endosome; Isopeptide bond; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation;
KW Vacuole.
FT CHAIN 1..133
FT /note="Protein SNA3"
FT /id="PRO_0000193990"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..48
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 89..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 125
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 133 AA; 15199 MW; DBA26AF5B769C02A CRC64;
MDRDHINDHD HRMSYSINKD DLLLMVLAVF IPPVAVWKRK GMFNRDTLLN LLLFLLLFFP
AIIHACYVVY ETSSERSYDL SRRHATAPAV DRDLEAHPAE ESQAQPPAYD EDDEAGADVP
LMDNKQQLSS GRT
