ID   SNAA_DICDI              Reviewed;         291 AA.
AC   Q54NP6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Alpha-soluble NSF attachment protein;
DE            Short=SNAP-alpha;
DE   AltName: Full=N-ethylmaleimide-sensitive factor attachment protein alpha;
GN   Name=snpA; ORFNames=DDB_G0285111;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 21-33 AND 252-259, SUBCELLULAR LOCATION, AND
RP   INTERACTION WITH NSFA.
RX   PubMed=10727946; DOI=10.1046/j.1432-1327.2000.01212.x;
RA   Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M.;
RT   "Functional and molecular identification of novel members of the ubiquitous
RT   membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-
RT   sensitive factor-attachment proteins) families in Dictyostelium
RT   discoideum.";
RL   Eur. J. Biochem. 267:2062-2070(2000).
RN   [3]
RP   FUNCTION, INTERACTION WITH SYN7A, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12175335; DOI=10.1042/bj20020845;
RA   Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J.,
RA   Satre M., Bruckert F.;
RT   "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane
RT   protein 7) form an active SNARE complex for early macropinocytic
RT   compartment fusion in Dictyostelium discoideum.";
RL   Biochem. J. 368:29-39(2002).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: May be required for vesicular transport between the
CC       endoplasmic reticulum and the Golgi apparatus (By similarity). Involved
CC       in vesicle fusion with nsfA and probably SNARE proteins. {ECO:0000250,
CC       ECO:0000269|PubMed:12175335}.
CC   -!- SUBUNIT: Interacts with nsfA and probably SNARE proteins.
CC       {ECO:0000269|PubMed:10727946, ECO:0000269|PubMed:12175335}.
CC   -!- INTERACTION:
CC       Q54NP6; Q75JI3: nsfA; NbExp=2; IntAct=EBI-1810173, EBI-1810142;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:10727946}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:10727946}.
CC   -!- SIMILARITY: Belongs to the SNAP family. {ECO:0000305}.
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DR   EMBL; AAFI02000074; EAL64868.1; -; Genomic_DNA.
DR   RefSeq; XP_639870.1; XM_634778.1.
DR   AlphaFoldDB; Q54NP6; -.
DR   SMR; Q54NP6; -.
DR   IntAct; Q54NP6; 2.
DR   STRING; 44689.DDB0231538; -.
DR   PaxDb; Q54NP6; -.
DR   EnsemblProtists; EAL64868; EAL64868; DDB_G0285111.
DR   GeneID; 8624941; -.
DR   KEGG; ddi:DDB_G0285111; -.
DR   dictyBase; DDB_G0285111; snpA.
DR   eggNOG; KOG1586; Eukaryota.
DR   HOGENOM; CLU_046329_0_2_1; -.
DR   InParanoid; Q54NP6; -.
DR   OMA; WSVKEYL; -.
DR   PhylomeDB; Q54NP6; -.
DR   Reactome; R-DDI-204005; COPII-mediated vesicle transport.
DR   Reactome; R-DDI-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-DDI-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   Reactome; R-DDI-6811438; Intra-Golgi traffic.
DR   Reactome; R-DDI-6811440; Retrograde transport at the Trans-Golgi-Network.
DR   PRO; PR:Q54NP6; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0005483; F:soluble NSF attachment protein activity; IDA:dictyBase.
DR   GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0035494; P:SNARE complex disassembly; IBA:GO_Central.
DR   CDD; cd15832; SNAP; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR000744; NSF_attach.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR13768; PTHR13768; 1.
DR   PRINTS; PR00448; NSFATTACHMNT.
DR   SMART; SM00028; TPR; 4.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; Direct protein sequencing; ER-Golgi transport;
KW   Membrane; Protein transport; Reference proteome; Repeat; TPR repeat;
KW   Transport.
FT   CHAIN           1..291
FT                   /note="Alpha-soluble NSF attachment protein"
FT                   /id="PRO_0000327891"
FT   REPEAT          14..51
FT                   /note="TPR 1"
FT   REPEAT          77..110
FT                   /note="TPR 2"
FT   REPEAT          117..150
FT                   /note="TPR 3"
FT   REPEAT          157..190
FT                   /note="TPR 4"
SQ   SEQUENCE   291 AA;  32407 MW;  39E43193BEE91120 CRC64;
     MGDDAKAKEF LDAADKRLRG GNFFKMFGGG SSRYDDAASD YTKAANLFKM SKKWDQAGAA
     FQKAAECFLK GSSKHDAASS YVLAAGCYKK GNVIDAITCL KAAIEYYTDE GRFAISAKHQ
     KEIAELYEAE GDFDQAIASY QIASDYFDGE NSTVSSHQCL LKIALFSAQL ERYEKSIEIY
     EQVAAASLDN NLTQWGCKEY FLRACLCYLA ADDVVGAERA LQRYKDMQAS FNSTRECRLL
     DGIIQACRNN NVEDFTNEVA EFNSISPLDS WKTSILLRIK NTINRETESV V