ID   SNAG_DICDI              Reviewed;         324 AA.
AC   Q9U9R7; Q55BN4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Gamma-soluble NSF attachment protein;
DE            Short=SNAP-gamma;
DE   AltName: Full=N-ethylmaleimide-sensitive factor attachment protein gamma;
GN   Name=snpC; ORFNames=DDB_G0269186;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND INTERACTION
RP   WITH NSFA.
RC   STRAIN=AX2;
RX   PubMed=10727946; DOI=10.1046/j.1432-1327.2000.01212.x;
RA   Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M.;
RT   "Functional and molecular identification of novel members of the ubiquitous
RT   membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-
RT   sensitive factor-attachment proteins) families in Dictyostelium
RT   discoideum.";
RL   Eur. J. Biochem. 267:2062-2070(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, INTERACTION WITH SYN7A, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12175335; DOI=10.1042/bj20020845;
RA   Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J.,
RA   Satre M., Bruckert F.;
RT   "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane
RT   protein 7) form an active SNARE complex for early macropinocytic
RT   compartment fusion in Dictyostelium discoideum.";
RL   Biochem. J. 368:29-39(2002).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: May be required for vesicular transport between the
CC       endoplasmic reticulum and the Golgi apparatus (By similarity). Involved
CC       in vesicle fusion with nsfA and probably SNARE proteins. {ECO:0000250,
CC       ECO:0000269|PubMed:12175335}.
CC   -!- SUBUNIT: Interacts with nsfA and probably SNARE proteins.
CC       {ECO:0000269|PubMed:10727946, ECO:0000269|PubMed:12175335}.
CC   -!- INTERACTION:
CC       Q9U9R7; Q75JI3: nsfA; NbExp=2; IntAct=EBI-1810184, EBI-1810142;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:10727946}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:10727946}.
CC   -!- SIMILARITY: Belongs to the SNAP family. {ECO:0000305}.
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DR   EMBL; AF157836; AAD45894.1; -; Genomic_DNA.
DR   EMBL; AAFI02000005; EAL71943.2; -; Genomic_DNA.
DR   RefSeq; XP_646797.2; XM_641705.2.
DR   AlphaFoldDB; Q9U9R7; -.
DR   SMR; Q9U9R7; -.
DR   IntAct; Q9U9R7; 2.
DR   STRING; 44689.DDB0191120; -.
DR   PaxDb; Q9U9R7; -.
DR   EnsemblProtists; EAL71943; EAL71943; DDB_G0269186.
DR   GeneID; 8617770; -.
DR   KEGG; ddi:DDB_G0269186; -.
DR   dictyBase; DDB_G0269186; snpC.
DR   eggNOG; KOG1585; Eukaryota.
DR   HOGENOM; CLU_063974_2_0_1; -.
DR   InParanoid; Q9U9R7; -.
DR   OMA; MHKENGN; -.
DR   PhylomeDB; Q9U9R7; -.
DR   Reactome; R-DDI-204005; COPII-mediated vesicle transport.
DR   Reactome; R-DDI-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-DDI-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   Reactome; R-DDI-6811438; Intra-Golgi traffic.
DR   Reactome; R-DDI-6811440; Retrograde transport at the Trans-Golgi-Network.
DR   PRO; PR:Q9U9R7; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0005483; F:soluble NSF attachment protein activity; IDA:dictyBase.
DR   GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR   CDD; cd15832; SNAP; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR000744; NSF_attach.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR13768; PTHR13768; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; ER-Golgi transport; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..324
FT                   /note="Gamma-soluble NSF attachment protein"
FT                   /id="PRO_0000327892"
FT   REGION          285..324
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        285..318
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   324 AA;  36734 MW;  489F586277AD967F CRC64;
     MSDKLKSNKK LEGETFMKEG DKLSKTNIFR WKADWDSAAL AYEKAANAFR SAKIYDSAKY
     CFLRLSLCQT HMDVYYLAAK SMENASAMAK ELKETQECAN LLLESCKLYR TNGNSFQAAD
     TMTKAAKLLE DIDLNQTIKL LTDACELFEL DDKDHFSGDT FKQTISMLLK HKKYTEAVDL
     MILQNRVFVK LEQNHDLHKS CLSVITISLA TDDIVASKKY YEQFLDYPSF IHSQEGTTAQ
     ELITAFDNHD VDGVKKIVSR HIFNFLDNQV AKIAKNLSIS KDSLNPTINS TAPQQQYSNT
     TTTTTNNTNN NNPTSQQDDD EDVL