SNAG_HUMAN
ID SNAG_HUMAN Reviewed; 312 AA.
AC Q99747; B4DFC9; Q9BUV1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Gamma-soluble NSF attachment protein {ECO:0000303|PubMed:11278501};
DE Short=SNAP-gamma {ECO:0000303|PubMed:11278501};
DE AltName: Full=N-ethylmaleimide-sensitive factor attachment protein gamma {ECO:0000312|HGNC:HGNC:7642};
GN Name=NAPG {ECO:0000312|HGNC:HGNC:7642};
GN Synonyms=SNAPG {ECO:0000250|UniProtKB:Q9CWZ7};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9269766;
RA Lemons P.P., Chen D., Bernstein A.M., Bennett M.K., Whiteheart S.W.;
RT "Regulated secretion in platelets: identification of elements of the
RT platelet exocytosis machinery.";
RL Blood 90:1490-1500(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH RAB11FIP5.
RX PubMed=11278501; DOI=10.1074/jbc.m009424200;
RA Chen D., Xu W., He P., Medrano E.E., Whiteheart S.W.;
RT "Gaf-1, a gamma-SNAP-binding protein associated with the mitochondria.";
RL J. Biol. Chem. 276:13127-13135(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15592455; DOI=10.1038/nbt1046;
RA Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,
RA Zha X.-M., Polakiewicz R.D., Comb M.J.;
RT "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.";
RL Nat. Biotechnol. 23:94-101(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Required for vesicular transport between the endoplasmic
CC reticulum and the Golgi apparatus.
CC -!- SUBUNIT: Interacts with RAB11FIP5 (PubMed:11278501). Interacts with
CC VTI1A (By similarity). {ECO:0000250|UniProtKB:Q9CWZ7,
CC ECO:0000269|PubMed:11278501}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P81127};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P81127}. Golgi
CC apparatus {ECO:0000250|UniProtKB:Q9CWZ7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99747-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99747-2; Sequence=VSP_056355;
CC -!- SIMILARITY: Belongs to the SNAP family. {ECO:0000305}.
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DR EMBL; U78107; AAB69753.1; -; mRNA.
DR EMBL; AK294038; BAG57390.1; -; mRNA.
DR EMBL; AP001099; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC001889; AAH01889.1; -; mRNA.
DR CCDS; CCDS45827.1; -. [Q99747-1]
DR RefSeq; NP_003817.1; NM_003826.2. [Q99747-1]
DR RefSeq; XP_011524058.1; XM_011525756.2. [Q99747-2]
DR AlphaFoldDB; Q99747; -.
DR SMR; Q99747; -.
DR BioGRID; 114304; 80.
DR CORUM; Q99747; -.
DR IntAct; Q99747; 32.
DR MINT; Q99747; -.
DR STRING; 9606.ENSP00000324628; -.
DR GlyGen; Q99747; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q99747; -.
DR PhosphoSitePlus; Q99747; -.
DR SwissPalm; Q99747; -.
DR BioMuta; NAPG; -.
DR DMDM; 3024632; -.
DR OGP; Q99747; -.
DR EPD; Q99747; -.
DR jPOST; Q99747; -.
DR MassIVE; Q99747; -.
DR MaxQB; Q99747; -.
DR PaxDb; Q99747; -.
DR PeptideAtlas; Q99747; -.
DR PRIDE; Q99747; -.
DR ProteomicsDB; 4024; -.
DR ProteomicsDB; 78455; -. [Q99747-1]
DR Antibodypedia; 2189; 186 antibodies from 28 providers.
DR DNASU; 8774; -.
DR Ensembl; ENST00000322897.11; ENSP00000324628.6; ENSG00000134265.13. [Q99747-1]
DR GeneID; 8774; -.
DR KEGG; hsa:8774; -.
DR MANE-Select; ENST00000322897.11; ENSP00000324628.6; NM_003826.3; NP_003817.1.
DR UCSC; uc002kon.4; human. [Q99747-1]
DR CTD; 8774; -.
DR DisGeNET; 8774; -.
DR GeneCards; NAPG; -.
DR HGNC; HGNC:7642; NAPG.
DR HPA; ENSG00000134265; Low tissue specificity.
DR MIM; 603216; gene.
DR neXtProt; NX_Q99747; -.
DR OpenTargets; ENSG00000134265; -.
DR PharmGKB; PA31445; -.
DR VEuPathDB; HostDB:ENSG00000134265; -.
DR eggNOG; KOG1585; Eukaryota.
DR GeneTree; ENSGT00390000005826; -.
DR HOGENOM; CLU_063974_1_0_1; -.
DR InParanoid; Q99747; -.
DR OMA; MHKENGN; -.
DR OrthoDB; 1540214at2759; -.
DR PhylomeDB; Q99747; -.
DR TreeFam; TF312872; -.
DR PathwayCommons; Q99747; -.
DR Reactome; R-HSA-204005; COPII-mediated vesicle transport.
DR Reactome; R-HSA-6807878; COPI-mediated anterograde transport.
DR Reactome; R-HSA-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-HSA-6811438; Intra-Golgi traffic.
DR Reactome; R-HSA-6811440; Retrograde transport at the Trans-Golgi-Network.
DR SignaLink; Q99747; -.
DR BioGRID-ORCS; 8774; 498 hits in 1081 CRISPR screens.
DR ChiTaRS; NAPG; human.
DR GeneWiki; NAPG; -.
DR GenomeRNAi; 8774; -.
DR Pharos; Q99747; Tbio.
DR PRO; PR:Q99747; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q99747; protein.
DR Bgee; ENSG00000134265; Expressed in endothelial cell and 191 other tissues.
DR ExpressionAtlas; Q99747; baseline and differential.
DR Genevisible; Q99747; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; HDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005483; F:soluble NSF attachment protein activity; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; TAS:ProtInc.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0061025; P:membrane fusion; TAS:ProtInc.
DR GO; GO:0050821; P:protein stabilization; NAS:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; NAS:UniProtKB.
DR CDD; cd15832; SNAP; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR000744; NSF_attach.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR13768; PTHR13768; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ER-Golgi transport; Golgi apparatus; Membrane;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..312
FT /note="Gamma-soluble NSF attachment protein"
FT /id="PRO_0000219063"
FT REGION 281..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 284
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9CWZ7"
FT MOD_RES 287
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9CWZ7"
FT MOD_RES 308
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9CWZ7"
FT VAR_SEQ 1..82
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056355"
FT VARIANT 92
FT /note="P -> S (in dbSNP:rs2228300)"
FT /id="VAR_052027"
FT VARIANT 281
FT /note="K -> N (in dbSNP:rs2305370)"
FT /id="VAR_020129"
FT CONFLICT 147
FT /note="E -> D (in Ref. 4; AAH01889)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 34746 MW; C37D73C1AEFD48C8 CRC64;
MAAQKINEGL EHLAKAEKYL KTGFLKWKPD YDSAASEYGK AAVAFKNAKQ FEQAKDACLR
EAVAHENNRA LFHAAKAYEQ AGMMLKEMQK LPEAVQLIEK ASMMYLENGT PDTAAMALER
AGKLIENVDP EKAVQLYQQT ANVFENEERL RQAVELLGKA SRLLVRGRRF DEAALSIQKE
KNIYKEIENY PTCYKKTIAQ VLVHLHRNDY VAAERCVRES YSIPGFNGSE DCAALEQLLE
GYDQQDQDQV SDVCNSPLFK YMDNDYAKLG LSLVVPGGGI KKKSPATPQA KPDGVTATAA
DEEEDEYSGG LC
