SNF12_YEAST
ID SNF12_YEAST Reviewed; 566 AA.
AC P53628; D6W1J8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transcription regulatory protein SNF12;
DE AltName: Full=SWI/SNF complex component SWP73;
GN Name=SNF12; Synonyms=SWP73; OrderedLocusNames=YNR023W; ORFNames=N3224;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Donald K.A.G., Hill J., Griffiths D.E.;
RL Submitted (NOV-1991) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBUNIT.
RX PubMed=8804308; DOI=10.1101/gad.10.17.2131;
RA Cairns B.R., Levinson R.S., Yamamoto K.R., Kornberg R.D.;
RT "Essential role of Swp73p in the function of yeast Swi/Snf complex.";
RL Genes Dev. 10:2131-2144(1996).
RN [5]
RP 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, AND ELECTRON MICROSCOPY OF
RP THE SWI/SNF COMPLEX.
RX PubMed=12524530; DOI=10.1038/nsb888;
RA Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L.,
RA Peterson C.L.;
RT "Structural analysis of the yeast SWI/SNF chromatin remodeling complex.";
RL Nat. Struct. Biol. 10:141-145(2003).
RN [6]
RP INTERACTION WITH SWP82.
RX PubMed=16204215; DOI=10.1534/genetics.105.047589;
RA Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.;
RT "The RSC chromatin remodeling complex bears an essential fungal-specific
RT protein module with broad functional roles.";
RL Genetics 172:795-809(2006).
CC -!- FUNCTION: Involved in transcriptional activation. Component of the
CC SWI/SNF complex, an ATP-dependent chromatin remodeling complex, which
CC is required for the positive and negative regulation of gene expression
CC of a large number of genes. It changes chromatin structure by altering
CC DNA-histone contacts within a nucleosome, leading eventually to a
CC change in nucleosome position, thus facilitating or repressing binding
CC of gene-specific transcription factors. {ECO:0000269|PubMed:8804308}.
CC -!- SUBUNIT: Interacts with SWP82. Component of the SWI/SNF global
CC transcription activator complex. The 1.14 MDa SWI/SNF complex is
CC composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2,
CC SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3,
CC SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
CC {ECO:0000269|PubMed:16204215, ECO:0000269|PubMed:8804308}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: To yeast RSC6. {ECO:0000305}.
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DR EMBL; X62430; CAA44296.1; -; Genomic_DNA.
DR EMBL; Z71638; CAA96302.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10564.1; -; Genomic_DNA.
DR PIR; S19063; S19063.
DR RefSeq; NP_014420.1; NM_001183200.1.
DR PDB; 6UXV; EM; 4.70 A; H=1-566.
DR PDB; 6UXW; EM; 8.96 A; H=1-566.
DR PDB; 7C4J; EM; 2.89 A; A=1-566.
DR PDB; 7EGM; EM; 3.60 A; H=1-566.
DR PDB; 7EGP; EM; 6.90 A; H=1-566.
DR PDBsum; 6UXV; -.
DR PDBsum; 6UXW; -.
DR PDBsum; 7C4J; -.
DR PDBsum; 7EGM; -.
DR PDBsum; 7EGP; -.
DR AlphaFoldDB; P53628; -.
DR SMR; P53628; -.
DR BioGRID; 35848; 156.
DR ComplexPortal; CPX-1150; SWI/SNF chromatin remodelling complex.
DR DIP; DIP-2443N; -.
DR IntAct; P53628; 27.
DR MINT; P53628; -.
DR STRING; 4932.YNR023W; -.
DR iPTMnet; P53628; -.
DR MaxQB; P53628; -.
DR PaxDb; P53628; -.
DR PRIDE; P53628; -.
DR EnsemblFungi; YNR023W_mRNA; YNR023W; YNR023W.
DR GeneID; 855757; -.
DR KEGG; sce:YNR023W; -.
DR SGD; S000005306; SNF12.
DR VEuPathDB; FungiDB:YNR023W; -.
DR eggNOG; KOG2570; Eukaryota.
DR GeneTree; ENSGT00940000176438; -.
DR HOGENOM; CLU_032070_0_0_1; -.
DR InParanoid; P53628; -.
DR OMA; PYFWNSD; -.
DR BioCyc; YEAST:G3O-33337-MON; -.
DR Reactome; R-SCE-3214858; RMTs methylate histone arginines.
DR PRO; PR:P53628; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53628; protein.
DR GO; GO:0000785; C:chromatin; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0016514; C:SWI/SNF complex; IDA:SGD.
DR GO; GO:0006338; P:chromatin remodeling; IDA:ComplexPortal.
DR GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; IC:ComplexPortal.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0061412; P:positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR InterPro; IPR036885; SWIB_MDM2_dom_sf.
DR SUPFAM; SSF47592; SSF47592; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..566
FT /note="Transcription regulatory protein SNF12"
FT /id="PRO_0000072005"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 196..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 56..59
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 65..68
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 70..105
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 122..132
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 158..164
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 170..172
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 178..181
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 184..189
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 266..268
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 280..283
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 292..298
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 312..315
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 321..323
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 325..338
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 356..361
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 404..414
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 421..426
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 429..433
FT /evidence="ECO:0007829|PDB:7C4J"
FT TURN 435..437
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 438..445
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 447..454
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 458..509
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 511..529
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 537..540
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 544..548
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 550..562
FT /evidence="ECO:0007829|PDB:7C4J"
SQ SEQUENCE 566 AA; 63871 MW; CD6E61EEEE85098C CRC64;
MSKVMKPSNG KGSRKSSKAA TPDTKNFFHA KKKDPVNQDK ANNASQITPT VPHSHPSDMV
IPDHLAELIP ELYSFQQLVD SEKRLDHFIH LRNLHMKRMV AQWERSKLSQ EFLYPHLNFP
NVKFLRIFIS NVSENQPWQM DTNNEADLMA LENATWTMRI EGRLLDNVQA NDPAREKFSS
FIESIVVDFK NKENDNVPST KFNAAPEENA TEGPSDKKLN LNLPLQFSLP NGDNSTTTNT
DQNNATMGEE TAKKDMSSTT PKLESVKWQY DPNNPVDFDG LDIKRVGSEN VECTISILRK
SSPEEPFMSY SPQLTAIIGL KSGTSHDAIF SIYKYIHLNE LLTNDESAFE NLMGNRNNHN
SNTSTSKMLD AASSQVSIVK LDTQLITLLP SSLKESSPDT MKLTDLLSLI NSTHLLPLQP
IEIDYTVRVD KASTYGELVL DIEVPDVNAL KFNNTQRESQ IGAAELNENA RELEQIKPKI
ALQDKEITSV LSNLHESNKR YRFFKKISED PVKALNECIA STSNALKVLS GDEGYNEDMV
RRANFYKENE AMLRENIEVI LSNGRM
