SNF6_YEAST
ID SNF6_YEAST Reviewed; 332 AA.
AC P18888; D3DKU3;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Transcription regulatory protein SNF6;
DE AltName: Full=SWI/SNF complex component SNF6;
GN Name=SNF6; OrderedLocusNames=YHL025W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2188093; DOI=10.1128/mcb.10.6.2544-2553.1990;
RA Estruch F., Carlson M.;
RT "SNF6 encodes a nuclear protein that is required for expression of many
RT genes in Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 10:2544-2553(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-165, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, AND ELECTRON MICROSCOPY OF
RP THE SWI/SNF COMPLEX.
RX PubMed=12524530; DOI=10.1038/nsb888;
RA Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L.,
RA Peterson C.L.;
RT "Structural analysis of the yeast SWI/SNF chromatin remodeling complex.";
RL Nat. Struct. Biol. 10:141-145(2003).
CC -!- FUNCTION: Involved in transcriptional activation. Component of the
CC SWI/SNF complex, an ATP-dependent chromatin remodeling complex, which
CC is required for the positive and negative regulation of gene expression
CC of a large number of genes. It changes chromatin structure by altering
CC DNA-histone contacts within a nucleosome, leading eventually to a
CC change in nucleosome position, thus facilitating or repressing binding
CC of gene-specific transcription factors.
CC -!- SUBUNIT: Component of the SWI/SNF global transcription activator
CC complex. The 1.14 MDa SWI/SNF complex is composed of 11 different
CC subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73,
CC ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82;
CC and three copies of TAF14/SWP29.
CC -!- INTERACTION:
CC P18888; P32628: RAD23; NbExp=2; IntAct=EBI-17550, EBI-14668;
CC P18888; P14736: RAD4; NbExp=2; IntAct=EBI-17550, EBI-14766;
CC P18888; P38956: SNF11; NbExp=5; IntAct=EBI-17550, EBI-17560;
CC P18888; P32591: SWI3; NbExp=6; IntAct=EBI-17550, EBI-18622;
CC P18888; P35189: TAF14; NbExp=4; IntAct=EBI-17550, EBI-18920;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Long;
CC IsoId=P18888-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P18888-2; Sequence=Not described;
CC -!- MISCELLANEOUS: Present with 2900 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- MISCELLANEOUS: [Isoform Short]: Partially functional. {ECO:0000305}.
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DR EMBL; M37132; AAA35063.1; -; Genomic_DNA.
DR EMBL; M32028; AAA35064.1; -; Genomic_DNA.
DR EMBL; U11582; AAB65078.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06660.1; -; Genomic_DNA.
DR PIR; A36313; A36313.
DR RefSeq; NP_011838.1; NM_001179105.1. [P18888-1]
DR PDB; 6UXV; EM; 4.70 A; I=1-150.
DR PDB; 6UXW; EM; 8.96 A; I=1-150.
DR PDB; 7C4J; EM; 2.89 A; C=1-332.
DR PDB; 7EGM; EM; 3.60 A; I=1-332.
DR PDB; 7EGP; EM; 6.90 A; I=1-332.
DR PDBsum; 6UXV; -.
DR PDBsum; 6UXW; -.
DR PDBsum; 7C4J; -.
DR PDBsum; 7EGM; -.
DR PDBsum; 7EGP; -.
DR AlphaFoldDB; P18888; -.
DR SMR; P18888; -.
DR BioGRID; 36397; 539.
DR ComplexPortal; CPX-1150; SWI/SNF chromatin remodelling complex.
DR DIP; DIP-1226N; -.
DR IntAct; P18888; 39.
DR MINT; P18888; -.
DR STRING; 4932.YHL025W; -.
DR iPTMnet; P18888; -.
DR MaxQB; P18888; -.
DR PaxDb; P18888; -.
DR PRIDE; P18888; -.
DR EnsemblFungi; YHL025W_mRNA; YHL025W; YHL025W. [P18888-1]
DR GeneID; 856360; -.
DR KEGG; sce:YHL025W; -.
DR SGD; S000001017; SNF6.
DR VEuPathDB; FungiDB:YHL025W; -.
DR eggNOG; ENOG502S1YQ; Eukaryota.
DR HOGENOM; CLU_051557_0_0_1; -.
DR InParanoid; P18888; -.
DR OMA; HLLANYI; -.
DR BioCyc; YEAST:G3O-31045-MON; -.
DR PRO; PR:P18888; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P18888; protein.
DR GO; GO:0000785; C:chromatin; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0016514; C:SWI/SNF complex; IDA:SGD.
DR GO; GO:0000182; F:rDNA binding; IDA:SGD.
DR GO; GO:0006338; P:chromatin remodeling; IDA:ComplexPortal.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IMP:SGD.
DR GO; GO:0006289; P:nucleotide-excision repair; IMP:SGD.
DR GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; IC:ComplexPortal.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative initiation; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..332
FT /note="Transcription regulatory protein SNF6"
FT /id="PRO_0000072006"
FT REGION 278..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 2..8
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 165
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT HELIX 70..84
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:7C4J"
FT TURN 97..99
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 103..122
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 128..132
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 139..153
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 170..177
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 184..190
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 197..209
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 213..215
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 232..243
FT /evidence="ECO:0007829|PDB:7C4J"
SQ SEQUENCE 332 AA; 37607 MW; ED9EA9F313F9079F CRC64;
MGVIKKKRSH HGKASRQQYY SGVQVGGVGS MGAINNNIPS LTSFAEENNY QYGYSGSSAG
MNGRSLTYAQ QQLNKQRQDF ERVRLRPEQL SNIIHDESDT ISFRSNLLKN FISSNDAFNM
LSLTTVPCDR IEKSRLFSEK TIRYLMQKQH EMKTQAAELQ EKPLTPLKYT KLIAAAEDGS
RSTKDMIDAV FEQDSHLRYQ PDGVVVHRDD PALVGKLRGD LREAPADYWT HAYRDVLAQY
HEAKERIRQK EVTAGEAQDE ASLQQQQQQD LQQQQQVVTT VASQSPHATA TEKEPVPAVV
DDPLENMFGD YSNEPFNTNF DDEFGDLDAV FF
