ID   SNF8_DANRE              Reviewed;         258 AA.
AC   Q5U3V9; A4VAK8; Q1L8C5;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Vacuolar-sorting protein SNF8;
DE   AltName: Full=ESCRT-II complex subunit VPS22;
GN   Name=snf8; ORFNames=si:dkey-220f10.1, zgc:101578;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 104-258.
RC   TISSUE=Blastula;
RX   PubMed=17490614; DOI=10.1016/j.bbrc.2007.04.116;
RA   O'Boyle S., Bree R.T., McLoughlin S., Grealy M., Byrnes L.;
RT   "Identification of zygotic genes expressed at the midblastula transition in
RT   zebrafish.";
RL   Biochem. Biophys. Res. Commun. 358:462-468(2007).
RN   [4]
RP   SEQUENCE REVISION TO N-TERMINUS OF FRAGMENT.
RA   O'Boyle S.T.;
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the endosomal sorting complex required for
CC       transport II (ESCRT-II), which is required for multivesicular body
CC       (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC       MVB pathway mediates delivery of transmembrane proteins into the lumen
CC       of the lysosome for degradation. The ESCRT-II complex is probably
CC       involved in the recruitment of the ESCRT-III complex (By similarity).
CC       {ECO:0000250|UniProtKB:Q96H20}.
CC   -!- SUBUNIT: Component of the endosomal sorting required for transport
CC       complex II (ESCRT-II), composed of SNF8, VPS25 and VPS36.
CC       {ECO:0000250|UniProtKB:Q96H20}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SNF8 family. {ECO:0000305}.
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DR   EMBL; CR926135; CAK05456.1; -; Genomic_DNA.
DR   EMBL; BC085373; AAH85373.1; -; mRNA.
DR   EMBL; AM422108; CAM12249.2; -; mRNA.
DR   RefSeq; NP_001007413.1; NM_001007412.1.
DR   AlphaFoldDB; Q5U3V9; -.
DR   SMR; Q5U3V9; -.
DR   STRING; 7955.ENSDARP00000075332; -.
DR   PaxDb; Q5U3V9; -.
DR   Ensembl; ENSDART00000080888; ENSDARP00000075332; ENSDARG00000058076.
DR   GeneID; 492771; -.
DR   KEGG; dre:492771; -.
DR   CTD; 11267; -.
DR   ZFIN; ZDB-GENE-041114-117; snf8.
DR   eggNOG; KOG3341; Eukaryota.
DR   GeneTree; ENSGT00390000007843; -.
DR   HOGENOM; CLU_070147_2_0_1; -.
DR   InParanoid; Q5U3V9; -.
DR   OMA; SNTEQGC; -.
DR   OrthoDB; 869548at2759; -.
DR   PhylomeDB; Q5U3V9; -.
DR   TreeFam; TF105950; -.
DR   Reactome; R-DRE-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   PRO; PR:Q5U3V9; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000058076; Expressed in granulocyte and 21 other tissues.
DR   GO; GO:0000814; C:ESCRT II complex; IBA:GO_Central.
DR   GO; GO:0048856; P:anatomical structure development; IEA:UniProt.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR016689; ESCRT-2_cplx_Snf8.
DR   InterPro; IPR040608; Snf8/Vps36.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12806; PTHR12806; 1.
DR   Pfam; PF04157; EAP30; 1.
DR   PIRSF; PIRSF017215; ESCRT2_Vps22; 1.
DR   SUPFAM; SSF46785; SSF46785; 2.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..258
FT                   /note="Vacuolar-sorting protein SNF8"
FT                   /id="PRO_0000215212"
FT   COILED          28..50
FT                   /evidence="ECO:0000255"
FT   CONFLICT        88
FT                   /note="S -> P (in Ref. 2; AAH85373)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        119
FT                   /note="I -> T (in Ref. 3; CAM12249)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   258 AA;  28850 MW;  FAD0433268F65F62 CRC64;
     MHRRGVGAGA IAKKKLAEAK YKERGSVLAE DQIAQMSKQL DTFKTHLEEF ASKHKQEIRK
     SSQFRVQFQE MCATIGVDPL ASGKGFWSEM LGVGDFYYEL GVQIIEVCLA LKHRNGGLIT
     LDELHHRVLK GRGKFAQDVS QDDLVRAIKK LKAMGNGFGM IPVGGTYLVQ SVPAELNMDH
     TVVLQLAEKK GYVTVSEIRE SLKWEKERAC HVLDHLLKEG LAWLDSQAAG EPQYWLPALF
     SELLSQDVTP EEANQMTP