ID   SNF8_DICDI              Reviewed;         246 AA.
AC   Q54RC4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Vacuolar-sorting protein SNF8;
DE   AltName: Full=ESCRT-II complex subunit VPS22;
DE   AltName: Full=Vacuolar protein-sorting-associated protein 22;
GN   Name=snf8; Synonyms=vps22; ORFNames=DDB_G0283203;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the endosomal sorting complex required for
CC       transport II (ESCRT-II), which is required for multivesicular body
CC       (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC       MVB pathway mediates delivery of transmembrane proteins into the lumen
CC       of the lysosome for degradation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the endosomal sorting complex required for
CC       transport II (ESCRT-II). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SNF8 family. {ECO:0000305}.
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DR   EMBL; AAFI02000051; EAL65840.1; -; Genomic_DNA.
DR   RefSeq; XP_639221.1; XM_634129.1.
DR   AlphaFoldDB; Q54RC4; -.
DR   SMR; Q54RC4; -.
DR   STRING; 44689.DDB0234025; -.
DR   PaxDb; Q54RC4; -.
DR   EnsemblProtists; EAL65840; EAL65840; DDB_G0283203.
DR   GeneID; 8623994; -.
DR   KEGG; ddi:DDB_G0283203; -.
DR   dictyBase; DDB_G0283203; vps22.
DR   eggNOG; KOG3341; Eukaryota.
DR   HOGENOM; CLU_070147_2_0_1; -.
DR   InParanoid; Q54RC4; -.
DR   OMA; SNTEQGC; -.
DR   PhylomeDB; Q54RC4; -.
DR   Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   PRO; PR:Q54RC4; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0000814; C:ESCRT II complex; ISS:dictyBase.
DR   GO; GO:0006605; P:protein targeting; ISS:dictyBase.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR016689; ESCRT-2_cplx_Snf8.
DR   InterPro; IPR040608; Snf8/Vps36.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12806; PTHR12806; 1.
DR   Pfam; PF04157; EAP30; 1.
DR   PIRSF; PIRSF017215; ESCRT2_Vps22; 1.
DR   SUPFAM; SSF46785; SSF46785; 2.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Endosome; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..246
FT                   /note="Vacuolar-sorting protein SNF8"
FT                   /id="PRO_0000367437"
FT   COILED          8..34
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   246 AA;  28277 MW;  96D05128CCCA600E CRC64;
     MRRGIGIQAA QKQTQTQKQL QNVSEQLNTE NINKIKEQLL VFKENLEIFA TKHKKDIIKN
     PEFRKYFQDM CNMIGVDPLA SNKGFWCQVL GVGDFYYTLG VQIIEICLKY RSSNGGLMEM
     DTLAEHLRKL RGKNSQEISC DDIECSISKL KVLGNGFNII KVSGGKKLVQ SVPCELNKDH
     TDIIILAQDN NASITQSLVI SKLKWSEERI NNVINFLLQE SMIWIDEQSN ETIYWFPSLW
     SNFSFE