SNF8_RAT
ID SNF8_RAT Reviewed; 258 AA.
AC Q5RK19;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Vacuolar-sorting protein SNF8;
DE AltName: Full=ELL-associated protein of 30 kDa;
DE AltName: Full=ESCRT-II complex subunit VPS22;
GN Name=Snf8; Synonyms=Eap30;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PROTEIN SEQUENCE OF 37-53; 135-146 AND 211-218, AND FUNCTION IN
RP TRANSCRIPTION.
RC TISSUE=Liver;
RX PubMed=10419521; DOI=10.1074/jbc.274.31.21981;
RA Schmidt A.E., Miller T., Schmidt S.L., Shiekhattar R., Shilatifard A.;
RT "Cloning and characterization of the EAP30 subunit of the ELL complex that
RT confers derepression of transcription by RNA polymerase II.";
RL J. Biol. Chem. 274:21981-21985(1999).
RN [3]
RP IDENTIFICATION IN A COMPLEX WITH ELL; VPS25 AND VPS36.
RX PubMed=11278625; DOI=10.1074/jbc.m010142200;
RA Kamura T., Burian D., Khalili H., Schmidt S.L., Sato S., Liu W.-J.,
RA Conrad M.N., Conaway R.C., Conaway J.W., Shilatifard A.;
RT "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a
RT role for yeast EAP-like proteins in regulation of gene expression by
RT glucose.";
RL J. Biol. Chem. 276:16528-16533(2001).
CC -!- FUNCTION: Required for degradation of both endocytosed EGF and EGFR,
CC but not for the EGFR ligand-mediated internalization (By similarity).
CC Component of the endosomal sorting complex required for transport II
CC (ESCRT-II), which is required for multivesicular body (MVB) formation
CC and sorting of endosomal cargo proteins into MVBs. The MVB pathway
CC mediates delivery of transmembrane proteins into the lumen of the
CC lysosome for degradation. The ESCRT-II complex is probably involved in
CC the recruitment of the ESCRT-III complex. The ESCRT-II complex may also
CC play a role in transcription regulation by participating in
CC derepression of transcription by RNA polymerase II, possibly via its
CC interaction with ELL.Required for the exosomal release of SDCBP, CD63
CC and syndecan (By similarity). {ECO:0000250|UniProtKB:Q96H20,
CC ECO:0000269|PubMed:10419521}.
CC -!- SUBUNIT: Interacts with TSG101 (via the C-terminal domain). Interacts
CC with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the
CC endosome membranes. Interacts with 14-3-3 proteins (By similarity).
CC Component of the endosomal sorting complex required for transport II
CC (ESCRT-II), composed of SNF8, VPS25 and VPS36 (PubMed:11278625). SNF8
CC is essential for the stability of the ESCRT-II complex. ESCRT-II
CC interacts with ELL (By similarity). {ECO:0000250|UniProtKB:Q96H20,
CC ECO:0000269|PubMed:11278625}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome membrane
CC {ECO:0000250}. Nucleus {ECO:0000305}. Late endosome membrane
CC {ECO:0000250}. Note=Recruited to the endosome membrane to participate
CC in vesicle formation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF8 family. {ECO:0000305}.
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DR EMBL; BC086364; AAH86364.1; -; mRNA.
DR RefSeq; NP_001007805.1; NM_001007804.1.
DR AlphaFoldDB; Q5RK19; -.
DR SMR; Q5RK19; -.
DR STRING; 10116.ENSRNOP00000008964; -.
DR jPOST; Q5RK19; -.
DR PaxDb; Q5RK19; -.
DR PRIDE; Q5RK19; -.
DR Ensembl; ENSRNOT00000008964; ENSRNOP00000008964; ENSRNOG00000006500.
DR GeneID; 287645; -.
DR KEGG; rno:287645; -.
DR UCSC; RGD:1310144; rat.
DR CTD; 11267; -.
DR RGD; 1310144; Snf8.
DR eggNOG; KOG3341; Eukaryota.
DR GeneTree; ENSGT00390000007843; -.
DR HOGENOM; CLU_070147_2_0_1; -.
DR InParanoid; Q5RK19; -.
DR OMA; SNTEQGC; -.
DR OrthoDB; 869548at2759; -.
DR PhylomeDB; Q5RK19; -.
DR TreeFam; TF105950; -.
DR Reactome; R-RNO-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR PRO; PR:Q5RK19; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000006500; Expressed in skeletal muscle tissue and 20 other tissues.
DR Genevisible; Q5RK19; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR GO; GO:0000814; C:ESCRT II complex; ISO:RGD.
DR GO; GO:0031902; C:late endosome membrane; ISO:RGD.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0055037; C:recycling endosome; ISO:RGD.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0016247; F:channel regulator activity; ISO:RGD.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:RGD.
DR GO; GO:0045022; P:early endosome to late endosome transport; ISO:RGD.
DR GO; GO:0032456; P:endocytic recycling; ISO:RGD.
DR GO; GO:1903543; P:positive regulation of exosomal secretion; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0045732; P:positive regulation of protein catabolic process; ISO:RGD.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0042176; P:regulation of protein catabolic process; ISO:RGD.
DR GO; GO:0061635; P:regulation of protein complex stability; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:1903772; P:regulation of viral budding via host ESCRT complex; ISO:RGD.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR016689; ESCRT-2_cplx_Snf8.
DR InterPro; IPR040608; Snf8/Vps36.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12806; PTHR12806; 1.
DR Pfam; PF04157; EAP30; 1.
DR PIRSF; PIRSF017215; ESCRT2_Vps22; 1.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Direct protein sequencing; Endosome; Membrane;
KW Methylation; Nucleus; Protein transport; Reference proteome; Transcription;
KW Transcription regulation; Transport.
FT CHAIN 1..258
FT /note="Vacuolar-sorting protein SNF8"
FT /id="PRO_0000215211"
FT COILED 27..53
FT /evidence="ECO:0000255"
FT MOD_RES 4
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q96H20"
SQ SEQUENCE 258 AA; 28884 MW; FD649E7BD794485E CRC64;
MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF ASKHKQEIRK
NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL GVQIIEVCLA LKHRNGGLIT
LEELHQQVLK GRGKFAQDVS QDDLIRAIKK LKALGTGFGI IPVGGTYLIQ SVPAELNMDH
TVVLQLAEKN GYVTVSEIKA SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF
TDLYSQEISA EEAKEVFP
