SNF8_SCHPO
ID SNF8_SCHPO Reviewed; 252 AA.
AC O94663;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Vacuolar-sorting protein dot2;
DE AltName: Full=Defective organization of telomere protein 2;
DE AltName: Full=ELL-associated protein of 30 kDa homolog dot2;
GN Name=dot2; ORFNames=SPBC651.05c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=11901107; DOI=10.1093/genetics/160.3.861;
RA Jin Y., Uzawa S., Cande W.Z.;
RT "Fission yeast mutants affecting telomere clustering and meiosis-specific
RT spindle pole body integrity.";
RL Genetics 160:861-876(2002).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15992541; DOI=10.1016/j.devcel.2005.04.016;
RA Jin Y., Mancuso J.J., Uzawa S., Cronembold D., Cande W.Z.;
RT "The fission yeast homolog of the human transcription factor EAP30 blocks
RT meiotic spindle pole body amplification.";
RL Dev. Cell 9:63-73(2005).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP FUNCTION.
RX PubMed=17660439; DOI=10.1099/mic.0.2007/006072-0;
RA Iwaki T., Onishi M., Ikeuchi M., Kita A., Sugiura R., Giga-Hama Y.,
RA Fukui Y., Takegawa K.;
RT "Essential roles of class E Vps proteins for sorting into multivesicular
RT bodies in Schizosaccharomyces pombe.";
RL Microbiology 153:2753-2764(2007).
CC -!- FUNCTION: Component of the endosomal sorting complex required for
CC transport II (ESCRT-II), which is required for multivesicular body
CC (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC MVB pathway mediates delivery of transmembrane proteins into the lumen
CC of the lysosome for degradation. The ESCRT-II complex is probably
CC involved in the recruitment of the ESCRT-III complex. Negatively
CC regulates meiotic spindle pole body maturation via indirect regulation
CC of the pcp1 gene. Required for efficient entry into pre-meiotic S
CC phase. {ECO:0000269|PubMed:11901107, ECO:0000269|PubMed:15992541,
CC ECO:0000269|PubMed:17660439}.
CC -!- SUBUNIT: Component of the endosomal sorting complex required for
CC transport II (ESCRT-II). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF8 family. {ECO:0000305}.
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DR EMBL; CU329671; CAB37601.1; -; Genomic_DNA.
DR PIR; T40604; T40604.
DR RefSeq; NP_595503.1; NM_001021413.2.
DR AlphaFoldDB; O94663; -.
DR SMR; O94663; -.
DR BioGRID; 277662; 95.
DR IntAct; O94663; 1.
DR STRING; 4896.SPBC651.05c.1; -.
DR iPTMnet; O94663; -.
DR MaxQB; O94663; -.
DR PaxDb; O94663; -.
DR PRIDE; O94663; -.
DR EnsemblFungi; SPBC651.05c.1; SPBC651.05c.1:pep; SPBC651.05c.
DR GeneID; 2541147; -.
DR KEGG; spo:SPBC651.05c; -.
DR PomBase; SPBC651.05c; dot2.
DR VEuPathDB; FungiDB:SPBC651.05c; -.
DR eggNOG; KOG3341; Eukaryota.
DR HOGENOM; CLU_070147_0_0_1; -.
DR InParanoid; O94663; -.
DR OMA; SNTEQGC; -.
DR PhylomeDB; O94663; -.
DR Reactome; R-SPO-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR PRO; PR:O94663; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000814; C:ESCRT II complex; ISO:PomBase.
DR GO; GO:0033620; C:Mei2 nuclear dot complex; IDA:PomBase.
DR GO; GO:0016604; C:nuclear body; IDA:PomBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:PomBase.
DR GO; GO:0045324; P:late endosome to vacuole transport; IMP:PomBase.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:PomBase.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR016689; ESCRT-2_cplx_Snf8.
DR InterPro; IPR040608; Snf8/Vps36.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12806; PTHR12806; 1.
DR Pfam; PF04157; EAP30; 1.
DR PIRSF; PIRSF017215; ESCRT2_Vps22; 1.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 3: Inferred from homology;
KW Cytoplasm; Endosome; Membrane; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..252
FT /note="Vacuolar-sorting protein dot2"
FT /id="PRO_0000362144"
SQ SEQUENCE 252 AA; 28653 MW; 607C62443FBAEF09 CRC64;
MRKRIGIGAL NDDEYLKQYE EVGNELIEQQ SDEIASQLST FQEALKTFAR EHATEIKQNS
QFRNTFVKLA LKIGLDPFVS GSDESAWAAV GMNEFYYQVA VRVIEVCYAT QMENGGLLSV
SQVCRFLNEE NEAFGHEWLR ETDVVRAVDS LAPLGPGFVL EKIAGKQYIR SLPLELNTDQ
NVVLEAVEIL GYVTISILRD NYAWERSRCI QVLNDLVSKS LLWIDSQGVE MAYWGASNLI
DDQAQRFLYE NF
