SNG1_YEAST
ID SNG1_YEAST Reviewed; 547 AA.
AC P46950; D6VUX9;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Nitrosoguanidine resistance protein SNG1;
GN Name=SNG1; OrderedLocusNames=YGR197C; ORFNames=G7591;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7753113; DOI=10.1016/0165-7992(95)90037-3;
RA Grey M., Pich C.T., Haase E., Brendel M.;
RT "SNG1 -- a new gene involved in nitrosoguanidine resistance in
RT Saccharomyces cerevisiae.";
RL Mutat. Res. 346:207-214(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7502584; DOI=10.1002/yea.320111110;
RA Guerreiro P., Maia e Silva A., Barreiros T., Arroyo J., Garcia-Gonzalez M.,
RA Garcia-Saez M.I., Rodrigues-Pousada C., Nombela C.;
RT "The complete sequence of a 9000 bp fragment of the right arm of
RT Saccharomyces cerevisiae chromosome VII contains four previously unknown
RT open reading frames.";
RL Yeast 11:1087-1091(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP INDUCTION.
RX PubMed=12529331; DOI=10.1074/jbc.m208549200;
RA Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.;
RT "A general strategy to uncover transcription factor properties identifies a
RT new regulator of drug resistance in yeast.";
RL J. Biol. Chem. 278:11427-11432(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: May function as a N-methyl-N'nitro-N-nitrosoguanidine (MNNG)
CC export permease.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Transcriptionally regulated by PDR8.
CC {ECO:0000269|PubMed:12529331}.
CC -!- SIMILARITY: To yeast YJR015W. {ECO:0000305}.
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DR EMBL; X74920; CAA52881.1; -; Genomic_DNA.
DR EMBL; X82775; CAA58016.1; -; Genomic_DNA.
DR EMBL; Z72982; CAA97224.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08290.1; -; Genomic_DNA.
DR PIR; S53920; S53920.
DR RefSeq; NP_011713.1; NM_001181326.1.
DR AlphaFoldDB; P46950; -.
DR SMR; P46950; -.
DR BioGRID; 33450; 232.
DR DIP; DIP-8148N; -.
DR IntAct; P46950; 1.
DR STRING; 4932.YGR197C; -.
DR iPTMnet; P46950; -.
DR PaxDb; P46950; -.
DR PRIDE; P46950; -.
DR EnsemblFungi; YGR197C_mRNA; YGR197C; YGR197C.
DR GeneID; 853111; -.
DR KEGG; sce:YGR197C; -.
DR SGD; S000003429; SNG1.
DR VEuPathDB; FungiDB:YGR197C; -.
DR eggNOG; ENOG502QUA0; Eukaryota.
DR GeneTree; ENSGT00940000176467; -.
DR HOGENOM; CLU_020178_0_1_1; -.
DR InParanoid; P46950; -.
DR OMA; LTMWAVG; -.
DR BioCyc; YEAST:G3O-30883-MON; -.
DR PRO; PR:P46950; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P46950; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015931; P:nucleobase-containing compound transport; IMP:SGD.
DR GO; GO:0060237; P:regulation of fungal-type cell wall organization; IMP:SGD.
DR GO; GO:0061091; P:regulation of phospholipid translocation; IGI:SGD.
DR InterPro; IPR022703; DUF3533.
DR Pfam; PF12051; DUF3533; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..547
FT /note="Nitrosoguanidine resistance protein SNG1"
FT /id="PRO_0000072007"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 35..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 531..547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 91
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT CONFLICT 117
FT /note="A -> V (in Ref. 1; CAA52881)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 547 AA; 61293 MW; D4421BA2DED55420 CRC64;
MTKSVGDEES QYIEDPSFAA AAAFTGGRDG VSYSNQRFAE GSGHSSDLAK SLEDYRPPDE
KPSSLSSVGE GGANEEEKGG NDGGPLARIQ TGLFSPRLRN HRKKILSKFV LNNFFIACVC
VSLISIYWGA CYGTDRYFFK VKNIVVLQDA PSNTSVQSIS AIIPSLLASV PGTWHIYNAT
SFHRKFGTTN STEIDRKIVD LIYDERYWLA LNVKPNATDT LYNSLISQDA NSEFNSSIFF
ESVFESGRDP SSVKSTILPL MQQLEVRLQK YYVKEYLPSL MSNITSNDRD LNINMENWAI
AGQLLFTYND YRPFADRILM APLQVGLIYC ILLTVLQLSL YGKLHGEMAR VLKPKHILIY
RLLISWATYF LLSIGFCTVS AIFRIDFTPA FGRGGFVVYW MSTWLVMMAV GGANENVLSL
VIAYCPPYLS IWLMTWIILN ISASFYPMVL NNEFYRYGYI MPIHNAVDIY KVIFLNLTKR
KMGRNYGILV AWVALNTSLM PFCMKFAGKK MQKNAMQAAE AAVAAATQRA SRPAEANTDK
NNNPPGN
