SNI1_ARATH
ID SNI1_ARATH Reviewed; 432 AA.
AC Q9SWA6; Q8LGF4;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Negative regulator of systemic acquired resistance SNI1 {ECO:0000303|PubMed:10458608};
DE AltName: Full=Non-structural maintenance of chromosome element 5 {ECO:0000303|PubMed:24207055};
DE Short=Non-SMC element 5 {ECO:0000303|PubMed:24207055};
DE AltName: Full=Protein SUPPRESSOR OF NPR1, INDUCIBLE 1 {ECO:0000303|PubMed:10458608};
DE Short=Suppressor of npr1-1 {ECO:0000303|PubMed:10458608};
GN Name=SNI1 {ECO:0000303|PubMed:10458608};
GN Synonyms=NSE5 {ECO:0000303|PubMed:24207055};
GN OrderedLocusNames=At4g18470 {ECO:0000312|Araport:AT4G18470};
GN ORFNames=F28J12.3 {ECO:0000312|EMBL:AL021710};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10458608; DOI=10.1016/s0092-8674(00)81962-5;
RA Li X., Zhang Y., Clarke J.D., Li Y., Dong X.;
RT "Identification and cloning of a negative regulator of systemic acquired
RT resistance, SNI1, through a screen for suppressors of npr1-1.";
RL Cell 98:329-339(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=16766691; DOI=10.1105/tpc.105.039677;
RA Mosher R.A., Durrant W.E., Wang D., Song J., Dong X.;
RT "A comprehensive structure-function analysis of Arabidopsis SNI1 defines
RT essential regions and transcriptional repressor activity.";
RL Plant Cell 18:1750-1765(2006).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17369431; DOI=10.1104/pp.106.095299;
RA Kesarwani M., Yoo J., Dong X.;
RT "Genetic interactions of TGA transcription factors in the regulation of
RT pathogenesis-related genes and disease resistance in Arabidopsis.";
RL Plant Physiol. 144:336-346(2007).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17360504; DOI=10.1073/pnas.0609357104;
RA Durrant W.E., Wang S., Dong X.;
RT "Arabidopsis SNI1 and RAD51D regulate both gene transcription and DNA
RT recombination during the defense response.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:4223-4227(2007).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18321188; DOI=10.1094/mpmi-21-4-0424;
RA Wubben M.J., Jin J., Baum T.J.;
RT "Cyst nematode parasitism of Arabidopsis thaliana is inhibited by salicylic
RT acid (SA) and elicits uncoupled SA-independent pathogenesis-related gene
RT expression in roots.";
RL Mol. Plant Microbe Interact. 21:424-432(2008).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20935179; DOI=10.1104/pp.110.165563;
RA Pape S., Thurow C., Gatz C.;
RT "The Arabidopsis PR-1 promoter contains multiple integration sites for the
RT coactivator NPR1 and the repressor SNI1.";
RL Plant Physiol. 154:1805-1818(2010).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21149701; DOI=10.1073/pnas.1005978107;
RA Wang S., Durrant W.E., Song J., Spivey N.W., Dong X.;
RT "Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense
RT gene transcription during plant immune responses.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:22716-22721(2010).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH SSN2.
RX PubMed=21320694; DOI=10.1016/j.chom.2011.01.011;
RA Song J., Durrant W.E., Wang S., Yan S., Tan E.H., Dong X.;
RT "DNA repair proteins are directly involved in regulation of gene expression
RT during plant immune response.";
RL Cell Host Microbe 9:115-124(2011).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH NTL9/CBNAC.
RC STRAIN=cv. Columbia;
RX PubMed=22826500; DOI=10.1093/nar/gks683;
RA Kim H.S., Park H.C., Kim K.E., Jung M.S., Han H.J., Kim S.H., Kwon Y.S.,
RA Bahk S., An J., Bae D.W., Yun D.-J., Kwak S.-S., Chung W.S.;
RT "A NAC transcription factor and SNI1 cooperatively suppress basal pathogen
RT resistance in Arabidopsis thaliana.";
RL Nucleic Acids Res. 40:9182-9192(2012).
RN [14]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH RAD17.
RX PubMed=24207055; DOI=10.1016/j.molcel.2013.09.019;
RA Yan S., Wang W., Marques J., Mohan R., Saleh A., Durrant W.E., Song J.,
RA Dong X.;
RT "Salicylic acid activates DNA damage responses to potentiate plant
RT immunity.";
RL Mol. Cell 52:602-610(2013).
CC -!- FUNCTION: Component of the SMC5-SMC6 complex, a complex involved in
CC repair of DNA double-strand breaks by homologous recombination
CC (PubMed:24207055). Transcription repressor that prevents expression of
CC pathogenesis-related genes (PR) via histone modifications and binding
CC negative cis-acting elements at their promoters (PubMed:16766691,
CC PubMed:17369431, PubMed:20935179, PubMed:21320694). Negative regulator
CC of hypersensitive response (HR) and systemic acquired resistance (SAR)
CC required to dampen the basal expression of pathogenesis related (PR)
CC genes (PubMed:10458608, PubMed:16766691, PubMed:17360504,
CC PubMed:21149701). Functions synergistically with NTL9/CBNAC as negative
CC regulator of pathogen-induced PR1 expression and basal resistance to a
CC virulent strain of P.syringae (PubMed:22826500). Binds to the PR1 gene
CC promoter to suppress defense response in the absence of pathogen
CC challenge and is removed in response to induction (PubMed:21320694,
CC PubMed:22826500). Regulates negatively both gene expression and DNA
CC recombination during pathogen infection, thus being involved in short-
CC term defense response and a long-term survival strategy
CC (PubMed:17360504). Prevents effective immune responses that involve
CC activation of DNA damage responses, probably by negatively regulating
CC the DNA damage sensors RAD17 and ATR (PubMed:24207055). Negative
CC regulator of defenses against the beet cyst nematode H.schachtii
CC (PubMed:18321188). {ECO:0000269|PubMed:10458608,
CC ECO:0000269|PubMed:16766691, ECO:0000269|PubMed:17360504,
CC ECO:0000269|PubMed:17369431, ECO:0000269|PubMed:18321188,
CC ECO:0000269|PubMed:20935179, ECO:0000269|PubMed:21149701,
CC ECO:0000269|PubMed:21320694, ECO:0000269|PubMed:22826500,
CC ECO:0000269|PubMed:24207055}.
CC -!- SUBUNIT: Interacts with SSN2 (PubMed:21320694). Binds to NTL9/CBNAC to
CC promote its binding to promoters of target genes (PubMed:22826500).
CC Component of the SMC5-SMC6 complex which consists at least of SMC5 and
CC SMC6B. Interacts with RAD17 (PubMed:24207055).
CC {ECO:0000269|PubMed:21320694, ECO:0000269|PubMed:22826500,
CC ECO:0000269|PubMed:24207055}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10458608,
CC ECO:0000269|PubMed:16766691}. Note=Also detectable in some fluorescent
CC loci peripheral to the nucleus. {ECO:0000269|PubMed:16766691}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in the veins.
CC {ECO:0000269|PubMed:10458608}.
CC -!- DISRUPTION PHENOTYPE: Accumulation of DNA damage leading to
CC constitutively activated DNA damage responses (DDR) (PubMed:24207055).
CC Increased basal expression of pathogenesis-related (PR) genes (e.g.
CC PR1) and hypersensitive response (HR) (PubMed:16766691,
CC PubMed:17369431, PubMed:17360504, PubMed:20935179, PubMed:21149701,
CC PubMed:21320694). Chromatin modifications at the PR-1 promoter that
CC mimic induction (e.g. AcH3 and MeH3K4) (PubMed:16766691). Dwarf plants
CC with distorted leaves, reduced root length, early flowering and reduced
CC fertility (PubMed:16766691, PubMed:17360504, PubMed:21320694).
CC Decreased susceptibility to the beet cyst nematode H.schachtii
CC (PubMed:18321188). Mutant plants display enhanced resistance to the
CC bacterial pathogen P.syringae pv. tomato DC3000 (PubMed:22826500).
CC Constitutively elevated levels of somatic homologous recombination
CC (PubMed:17360504). Suppressor of the npr1-1 mutant phenotypes,
CC including systemic acquired resistance (SAR) and normal levels of PR1
CC restoration (PubMed:10458608, PubMed:20935179).
CC {ECO:0000269|PubMed:10458608, ECO:0000269|PubMed:16766691,
CC ECO:0000269|PubMed:17360504, ECO:0000269|PubMed:17369431,
CC ECO:0000269|PubMed:18321188, ECO:0000269|PubMed:20935179,
CC ECO:0000269|PubMed:21149701, ECO:0000269|PubMed:21320694,
CC ECO:0000269|PubMed:22826500, ECO:0000269|PubMed:24207055}.
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DR EMBL; AF169596; AAD50900.1; -; mRNA.
DR EMBL; AL021710; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE84048.1; -; Genomic_DNA.
DR EMBL; BT029212; ABJ17147.1; -; mRNA.
DR EMBL; AY084302; AAM60892.1; -; mRNA.
DR RefSeq; NP_567557.1; NM_117959.1.
DR AlphaFoldDB; Q9SWA6; -.
DR SMR; Q9SWA6; -.
DR IntAct; Q9SWA6; 6.
DR STRING; 3702.AT4G18470.1; -.
DR PaxDb; Q9SWA6; -.
DR PRIDE; Q9SWA6; -.
DR ProteomicsDB; 232476; -.
DR EnsemblPlants; AT4G18470.1; AT4G18470.1; AT4G18470.
DR GeneID; 827577; -.
DR Gramene; AT4G18470.1; AT4G18470.1; AT4G18470.
DR KEGG; ath:AT4G18470; -.
DR Araport; AT4G18470; -.
DR TAIR; locus:2124534; AT4G18470.
DR eggNOG; ENOG502RBSJ; Eukaryota.
DR HOGENOM; CLU_045759_1_0_1; -.
DR InParanoid; Q9SWA6; -.
DR OMA; CIAMQKF; -.
DR OrthoDB; 607346at2759; -.
DR PhylomeDB; Q9SWA6; -.
DR PRO; PR:Q9SWA6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SWA6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030915; C:Smc5-Smc6 complex; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:UniProtKB.
DR GO; GO:0002215; P:defense response to nematode; IMP:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0043966; P:histone H3 acetylation; IDA:UniProtKB.
DR GO; GO:0016570; P:histone modification; IMP:TAIR.
DR GO; GO:0031348; P:negative regulation of defense response; IMP:UniProtKB.
DR GO; GO:0051572; P:negative regulation of histone H3-K4 methylation; IDA:UniProtKB.
DR GO; GO:0010113; P:negative regulation of systemic acquired resistance; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR GO; GO:0016444; P:somatic cell DNA recombination; IMP:TAIR.
DR GO; GO:0009627; P:systemic acquired resistance; TAS:TAIR.
DR InterPro; IPR034561; SNI1.
DR PANTHER; PTHR37243; PTHR37243; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Hypersensitive response; Nucleus; Plant defense; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..432
FT /note="Negative regulator of systemic acquired resistance
FT SNI1"
FT /id="PRO_0000438367"
FT CONFLICT 142
FT /note="L -> F (in Ref. 5; AAM60892)"
FT /evidence="ECO:0000305"
FT CONFLICT 362
FT /note="I -> V (in Ref. 5; AAM60892)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 432 AA; 48812 MW; 2A577F53213B0C3D CRC64;
MSKETKGNNN TSRVMSGYGG SLEANTLAMI DSTGAKDSRD ANEDRLQYLE AVRAASLVPE
NGIPPTNKMY QAIFRILRFG KTLELITASF QLLTQLHQRF PWVYVSDSAD QLDIVDEAWS
PFNFGSDVDS DEKDLSVRSL FLQQLIQNMN KRVNESEESD LKILGNMFLF KYLAHVLKLD
FTPRNQVYEE TMNWSLLKES FLNLLLASRK VNFKLLMKDY LSTMCASIDA DEKSISLVEL
HKDMLTAMKE LLVMIMELDT SKKKADLEGI TSRGDGVRTP AMEIILDELT YDGYLLSKFL
QVFDDPKWKL EIVLQYLTKY IPKPVVRTRR TTVPQAEDSK TLNGILKTFS NGTNPKNITK
KIGPDIVQIL IGHAFLARLT FSDPHEGDSI SEICSSIISA FTSLKRVDQK IEILPFGKEV
LFTAGMVLKA KA
