SNKY_DROME
ID SNKY_DROME Reviewed; 715 AA.
AC Q9VU52;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 3.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Protein sneaky {ECO:0000305|PubMed:9630751};
GN Name=snky {ECO:0000303|PubMed:9630751, ECO:0000312|FlyBase:FBgn0086916};
GN ORFNames=CG11281 {ECO:0000312|FlyBase:FBgn0086916};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=9630751; DOI=10.1006/dbio.1997.8852;
RA Fitch K.R., Wakimoto B.T.;
RT "The paternal effect gene ms(3)sneaky is required for sperm activation and
RT the initiation of embryogenesis in Drosophila melanogaster.";
RL Dev. Biol. 197:270-282(1998).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17092953; DOI=10.1242/dev.02671;
RA Wilson K.L., Fitch K.R., Bafus B.T., Wakimoto B.T.;
RT "Sperm plasma membrane breakdown during Drosophila fertilization requires
RT sneaky, an acrosomal membrane protein.";
RL Development 133:4871-4879(2006).
CC -!- FUNCTION: Component of the sperm acrosome membrane (PubMed:17092953).
CC Required for breakdown of the sperm plasma membrane after sperm entry
CC into the egg, which is an essential prerequisite for successful
CC fertilization (PubMed:9630751, PubMed:17092953).
CC {ECO:0000269|PubMed:17092953, ECO:0000269|PubMed:9630751}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC membrane {ECO:0000269|PubMed:17092953}; Multi-pass membrane protein
CC {ECO:0000255}. Cytoplasm {ECO:0000269|PubMed:17092953}. Cytoplasmic
CC vesicle membrane {ECO:0000269|PubMed:17092953}; Multi-pass membrane
CC protein {ECO:0000255}. Note=Has diffuse cytoplasmic expression in
CC primary spermatocytes. In post-meiotic spermatids, detected in clusters
CC of vesicle-like structures adjacent to the nucleus. In late spermatids,
CC a single vesicle-like spot is found near the nucleus, which probably
CC corresponds to the acroblast. In mature sperm, localizes to the apical
CC tip of the sperm head (acrosome). {ECO:0000269|PubMed:17092953}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis.
CC {ECO:0000269|PubMed:17092953}.
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DR EMBL; AE014296; AAF49838.3; -; Genomic_DNA.
DR RefSeq; NP_648633.2; NM_140376.2.
DR AlphaFoldDB; Q9VU52; -.
DR STRING; 7227.FBpp0306188; -.
DR PaxDb; Q9VU52; -.
DR EnsemblMetazoa; FBtr0334063; FBpp0306188; FBgn0086916.
DR GeneID; 39492; -.
DR KEGG; dme:Dmel_CG11281; -.
DR UCSC; CG11281-RA; d. melanogaster.
DR CTD; 39492; -.
DR FlyBase; FBgn0086916; snky.
DR VEuPathDB; VectorBase:FBgn0086916; -.
DR eggNOG; KOG3726; Eukaryota.
DR GeneTree; ENSGT00940000153269; -.
DR HOGENOM; CLU_015030_1_1_1; -.
DR InParanoid; Q9VU52; -.
DR OMA; MAKPFTN; -.
DR OrthoDB; 572860at2759; -.
DR BioGRID-ORCS; 39492; 0 hits in 1 CRISPR screen.
DR ChiTaRS; snky; fly.
DR GenomeRNAi; 39492; -.
DR PRO; PR:Q9VU52; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0086916; Expressed in testis and 7 other tissues.
DR Genevisible; Q9VU52; DM.
DR GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:FlyBase.
DR GO; GO:0008270; F:zinc ion binding; ISM:FlyBase.
DR GO; GO:0035042; P:fertilization, exchange of chromosomal proteins; IMP:FlyBase.
DR GO; GO:0035041; P:sperm DNA decondensation; IMP:FlyBase.
DR GO; GO:0035045; P:sperm plasma membrane disassembly; IMP:FlyBase.
DR InterPro; IPR012858; DC_STAMP-like.
DR Pfam; PF07782; DC_STAMP; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoplasmic vesicle; Fertilization; Membrane; Metal-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT CHAIN 1..715
FT /note="Protein sneaky"
FT /id="PRO_0000445380"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..66
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..109
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..373
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 479..553
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 554..574
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..715
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT ZN_FING 655..691
FT /note="RING-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ SEQUENCE 715 AA; 83340 MW; 95F3011BC4E1902B CRC64;
MLSLLTRPFL PIFCFLYGPQ SEGSTRIQCL RRFVTFLLGL VLGFLLWKLA ALNFTLGRLF
VNGATDLYVF IIFVLVTGTI FMLSLPVRAV ILLIFVALVG KSGRTYLRAV AFAFIISGPI
ANLVENAGEV ARVFVCTTVL TYNLSKTRFD LMAKPFTNTL KHMRGDVEEI RHTFYELQEV
LVDLKYAVEN SDIEDEKYGD KNTKPIYERW GRETSRMNVS EIGNGGKELP TPAAVQERFQ
RNMRNRCKHQ LRSGHRACLE VFRNGYRKCT TNFPSMIAKA ICWPYRVDII CELDLFGNPD
KICDPSAVVP QNFGETYVEL LKAEKKLFDN SSQIVVNYEI KDEQFAKSQL KSAERTGQAF
KEDFERQKRI FNKVMGILQK ILCLFMLRMV YVSINYYVKY LNDVEFDNFY ITKYFKHVDQ
RRKEQRIDAI LPLRTYEKSK YIDVDHIFSR THHESTTVCF NLLQFLLELV TAGLFILIDH
LVVELLQIVR KRSKIVYQQD GEHEVRFNIS GVGQMARLLR TTMHNFNIHE KVSTSLSNKE
CLPNAHVLPK KMYYQLILLY LIIIVLIYQS TTFLRMRRVI CSFFYYKREK QRILFLYNRI
LRNRLRSLEF LIHDAEDNLA THRIQQQVNV FLWLRFSCPV AFGWIRHFKF AKRTCMICRG
LEDSTFTVCG NCGLPYCDDC AEDLNSVCFQ CGVVLTRGAE GSESSVEVYT YRKEK
