SNPC4_MOUSE
ID SNPC4_MOUSE Reviewed; 1333 AA.
AC Q8BP86; Q6PGG7; Q80UG9; Q810L1;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=snRNA-activating protein complex subunit 4;
DE Short=SNAPc subunit 4;
DE AltName: Full=snRNA-activating protein complex 190 kDa subunit;
DE Short=SNAPc 190 kDa subunit;
GN Name=Snapc4 {ECO:0000312|MGI:MGI:2443935};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAC36843.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC36843.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:BAC36843.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAH57031.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 498-1325 (ISOFORM 1).
RC STRAIN=129 {ECO:0000312|EMBL:AAH44754.1},
RC C57BL/6J {ECO:0000312|EMBL:AAH57031.1}, and FVB/N;
RC TISSUE=Brain {ECO:0000312|EMBL:AAH57031.1}, and
RC Mammary gland {ECO:0000312|EMBL:AAH44754.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1301 AND SER-1309, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Part of the SNAPc complex required for the transcription of
CC both RNA polymerase II and III small-nuclear RNA genes. Binds to the
CC proximal sequence element (PSE), a non-TATA-box basal promoter element
CC common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA
CC TATA box (By similarity). {ECO:0000250|UniProtKB:Q5SXM2}.
CC -!- SUBUNIT: Part of the SNAPc composed of 5 subunits: SNAPC1, SNAPC2,
CC SNAPC3, SNAPC4 and SNAPC5. SNAPC4 interacts with SNAPC1, SNAPC2,
CC SNAPC5, BRF2 and TBP (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000305};
CC IsoId=Q8BP86-1; Sequence=Displayed;
CC Name=2 {ECO:0000305};
CC IsoId=Q8BP86-2; Sequence=VSP_051857;
CC Name=3 {ECO:0000305};
CC IsoId=Q8BP86-3; Sequence=VSP_051855, VSP_051857;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK077522; BAC36843.1; -; mRNA.
DR EMBL; BC044754; AAH44754.1; -; mRNA.
DR EMBL; BC057031; AAH57031.1; -; mRNA.
DR CCDS; CCDS15802.1; -. [Q8BP86-2]
DR RefSeq; NP_001277348.1; NM_001290419.1. [Q8BP86-3]
DR RefSeq; NP_758842.1; NM_172339.4. [Q8BP86-2]
DR AlphaFoldDB; Q8BP86; -.
DR SMR; Q8BP86; -.
DR BioGRID; 230655; 3.
DR IntAct; Q8BP86; 2.
DR STRING; 10090.ENSMUSP00000109750; -.
DR iPTMnet; Q8BP86; -.
DR PhosphoSitePlus; Q8BP86; -.
DR MaxQB; Q8BP86; -.
DR PaxDb; Q8BP86; -.
DR PRIDE; Q8BP86; -.
DR ProteomicsDB; 261391; -. [Q8BP86-1]
DR ProteomicsDB; 261392; -. [Q8BP86-2]
DR ProteomicsDB; 261393; -. [Q8BP86-3]
DR Antibodypedia; 18701; 195 antibodies from 20 providers.
DR DNASU; 227644; -.
DR Ensembl; ENSMUST00000035427; ENSMUSP00000041767; ENSMUSG00000036281. [Q8BP86-2]
DR GeneID; 227644; -.
DR KEGG; mmu:227644; -.
DR UCSC; uc008iuu.2; mouse. [Q8BP86-1]
DR UCSC; uc008iuv.2; mouse. [Q8BP86-2]
DR UCSC; uc008iuw.2; mouse. [Q8BP86-3]
DR CTD; 6621; -.
DR MGI; MGI:2443935; Snapc4.
DR VEuPathDB; HostDB:ENSMUSG00000036281; -.
DR eggNOG; KOG0049; Eukaryota.
DR GeneTree; ENSGT00940000160404; -.
DR HOGENOM; CLU_004641_0_0_1; -.
DR InParanoid; Q8BP86; -.
DR OMA; LWHGTFQ; -.
DR OrthoDB; 219341at2759; -.
DR PhylomeDB; Q8BP86; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-MMU-76071; RNA Polymerase III Transcription Initiation From Type 3 Promoter.
DR BioGRID-ORCS; 227644; 28 hits in 77 CRISPR screens.
DR ChiTaRS; Snapc4; mouse.
DR PRO; PR:Q8BP86; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BP86; protein.
DR Bgee; ENSMUSG00000036281; Expressed in superior frontal gyrus and 200 other tissues.
DR ExpressionAtlas; Q8BP86; baseline and differential.
DR Genevisible; Q8BP86; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019185; C:snRNA-activating protein complex; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0001006; F:RNA polymerase III type 3 promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0042795; P:snRNA transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0042796; P:snRNA transcription by RNA polymerase III; ISS:UniProtKB.
DR CDD; cd00167; SANT; 3.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR SMART; SM00717; SANT; 5.
DR SUPFAM; SSF46689; SSF46689; 3.
DR PROSITE; PS51294; HTH_MYB; 3.
DR PROSITE; PS50090; MYB_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..1333
FT /note="snRNA-activating protein complex subunit 4"
FT /id="PRO_0000197121"
FT DOMAIN 250..288
FT /note="Myb-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT DOMAIN 289..343
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 344..395
FT /note="Myb-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT DOMAIN 396..451
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 452..503
FT /note="HTH myb-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 317..341
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 424..447
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 476..499
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 29..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 84..133
FT /note="SNAPC5-binding"
FT /evidence="ECO:0000250|UniProtKB:Q5SXM2"
FT REGION 503..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 811..842
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1079..1117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1131..1247
FT /note="SNAPC2-binding"
FT /evidence="ECO:0000250|UniProtKB:Q5SXM2"
FT REGION 1282..1333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..549
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 672..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 811..828
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 68
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SXM2"
FT MOD_RES 1252
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SXM2"
FT MOD_RES 1254
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SXM2"
FT MOD_RES 1301
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1309
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_051855"
FT VAR_SEQ 1290..1297
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_051857"
FT CONFLICT 498..505
FT /note="LARKKQHL -> DAWADAWV (in Ref. 2; AAH44754)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1333 AA; 147413 MW; 586798D3A7D59B4E CRC64;
MDIDAEREKI TQEIQELERI LYPGSTSVHF EVSESSLSSD SEADSLPDED LETAGAPILE
EEGSSESSND EEDPKDKALP EDPETCLQLN MVYQEVIREK LAEVSQLLAQ NQEQQEEILF
DLSGTKCPKV KDGRSLPSYM YIGHFLKPYF KDKVTGVGPP ANEETREKAT QGIKAFEQLL
VTKWKHWEKA LLRKSVVSDR LQRLLQPKLL KLEYLHEKQS RVSSELERQA LEKQIKEAEK
EIQDINQLPE EALLGNRLDS HDWEKISNIN FEGARSAEEI RKFWQSSEHP SISKQEWSTE
EVERLKAIAA THGHLEWHLV AEELGTSRSA FQCLQKFQQY NKTLKRKEWT EEEDHMLTQL
VQEMRVGNHI PYRKIVYFME GRDSMQLIYR WTKSLDPSLK RGFWAPEEDA KLLQAVAKYG
AQDWFKIREE VPGRSDAQCR DRYIRRLHFS LKKGRWNAKE EQQLIQLIEK YGVGHWARIA
SELPHRSGSQ CLSKWKILAR KKQHLQRKRG QRPRHSSQWS SSGSSSSSSE DYGSSSGSDG
SSGSENSDVE LEASLEKSRA LTPQQYRVPD IDLWVPTRLI TSQSQREGTG CYPQHPAVSC
CTQDASQNHH KEGSTTVSAA EKNQLQVPYE THSTVPRGDR FLHFSDTHSA SLKDPACKSH
TLMKERPKQP LLPSSRSGSD PGNNTAGPHL RQLWHGTYQN KQRRKRQALH RRLLKHRLLL
AVIPWVGDIN LACTQAPRRP ATVQTKADSI RMQLECARLA STPVFTLLIQ LLQIDTAGCM
EVVRERKSQP PALLQPGTRN TQPHLLQASS NAKNNTGCLP SMTGEQTAKR ASHKGRPRLG
SCRTEATPFQ VPVAAPRGLR PKPKTVSELL REKRLRESHA KKATQALGLN SQLLVSSPVI
LQPPLLPVPH GSPVVGPATS SVELSVPVAP VMVSSSPSGS WPVGGISATD KQPPNLQTIS
LNPPHKGTQV AAPAAFRSLA LAPGQVPTGG HLSTLGQTST TSQKQSLPKV LPILRAAPSL
TQLSVQPPVS GQPLATKSSL PVNWVLTTQK LLSVQVPAVV GLPQSVMTPE TIGLQAKQLP
SPAKTPAFLE QPPASTDTEP KGPQGQEIPP TPGPEKAALD LSLLSQESEA AIVTWLKGCQ
GAFVPPLGSR MPYHPPSLCS LRALSSLLLQ KQDLEQKASS LAASQAAGAQ PDPKAGALQA
SLELVQRQFR DNPAYLLLKT RFLAIFSLPA FLATLPPNSI PTTLSPDVAV VSESDSEDLG
DLELKDRARQ LDCMACRVQA SPAAPDPVQS HLVSPGQRAP SPGEVSAPSP LDASDGLDDL
NVLRTRRARH SRR
