SNR27_MOUSE
ID SNR27_MOUSE Reviewed; 155 AA.
AC Q8K194; Q8BRA8; Q9CSV0;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein;
DE Short=U4/U6.U5 snRNP 27 kDa protein;
DE Short=U4/U6.U5-27K;
DE AltName: Full=U4/U6.U5 tri-snRNP-associated protein 3;
GN Name=Snrnp27;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in mRNA splicing.
CC -!- SUBUNIT: Part of a tri-snRNP complex.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K194-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K194-2; Sequence=VSP_017317;
CC -!- SIMILARITY: Belongs to the SNUT3 family. {ECO:0000305}.
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DR EMBL; AK011937; BAB27926.2; -; mRNA.
DR EMBL; AK045231; BAC32271.1; -; mRNA.
DR EMBL; AK160190; BAE35683.1; -; mRNA.
DR EMBL; BC027564; AAH27564.1; -; mRNA.
DR CCDS; CCDS20315.1; -. [Q8K194-1]
DR RefSeq; NP_079941.1; NM_025665.2. [Q8K194-1]
DR AlphaFoldDB; Q8K194; -.
DR BioGRID; 211600; 16.
DR IntAct; Q8K194; 1.
DR MINT; Q8K194; -.
DR STRING; 10090.ENSMUSP00000001186; -.
DR iPTMnet; Q8K194; -.
DR PhosphoSitePlus; Q8K194; -.
DR EPD; Q8K194; -.
DR MaxQB; Q8K194; -.
DR PaxDb; Q8K194; -.
DR PeptideAtlas; Q8K194; -.
DR PRIDE; Q8K194; -.
DR ProteomicsDB; 261296; -. [Q8K194-1]
DR ProteomicsDB; 261297; -. [Q8K194-2]
DR Antibodypedia; 31048; 56 antibodies from 15 providers.
DR DNASU; 66618; -.
DR Ensembl; ENSMUST00000001186; ENSMUSP00000001186; ENSMUSG00000001158. [Q8K194-1]
DR Ensembl; ENSMUST00000113683; ENSMUSP00000109313; ENSMUSG00000001158. [Q8K194-2]
DR GeneID; 66618; -.
DR KEGG; mmu:66618; -.
DR UCSC; uc009csj.1; mouse. [Q8K194-1]
DR UCSC; uc009csk.1; mouse. [Q8K194-2]
DR CTD; 11017; -.
DR MGI; MGI:1913868; Snrnp27.
DR VEuPathDB; HostDB:ENSMUSG00000001158; -.
DR eggNOG; KOG3263; Eukaryota.
DR GeneTree; ENSGT00940000163817; -.
DR HOGENOM; CLU_075596_2_1_1; -.
DR InParanoid; Q8K194; -.
DR OMA; SMKRKYR; -.
DR OrthoDB; 1627375at2759; -.
DR TreeFam; TF314458; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 66618; 18 hits in 75 CRISPR screens.
DR ChiTaRS; Snrnp27; mouse.
DR PRO; PR:Q8K194; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8K194; protein.
DR Bgee; ENSMUSG00000001158; Expressed in embryonic brain and 262 other tissues.
DR Genevisible; Q8K194; MM.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISO:MGI.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR013957; SNRNP27.
DR Pfam; PF08648; SNRNP27; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..155
FT /note="U4/U6.U5 small nuclear ribonucleoprotein 27 kDa
FT protein"
FT /id="PRO_0000223966"
FT REGION 1..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..61
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..96
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WVK2"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WVK2"
FT MOD_RES 111
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WVK2"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WVK2"
FT MOD_RES 132
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WVK2"
FT VAR_SEQ 139..155
FT /note="QYMNRKGGFNRPLDFIA -> YACSPSCVCLRLERNVTTGLLHVGHVDIFHS
FT LHVGLCCPNVGITSICHQDWLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_017317"
SQ SEQUENCE 155 AA; 18885 MW; C98F1A310FD75DB7 CRC64;
MGRSRSRSPR RERRRSRSTS RDRERRRRER SRSRERDRRR SRSRSPHRRR SRSPRRHRST
SPSPSRLKER RDEEKKETKE IKNKERQITE EDLEGKTEEE IEMMKLMGFA SFDSTKGKKV
DGSVNAYAIN VSQKRKYRQY MNRKGGFNRP LDFIA
