SNR40_MOUSE
ID SNR40_MOUSE Reviewed; 358 AA.
AC Q6PE01; Q3TEJ3; Q9DCX3;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=U5 small nuclear ribonucleoprotein 40 kDa protein;
DE Short=U5 snRNP 40 kDa protein;
DE AltName: Full=WD repeat-containing protein 57;
GN Name=Snrnp40; Synonyms=Wdr57;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Kidney, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-21, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC spliceosome. Component of the U5 small nuclear ribonucleoprotein
CC (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of
CC the spliceosome. {ECO:0000250|UniProtKB:Q96DI7}.
CC -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC complexes. Component of the postcatalytic spliceosome P complex. Part
CC of the U5 snRNP complex. Interacts with PRPF8. Component of the U4/U6-
CC U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least
CC PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40,
CC DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39.
CC {ECO:0000250|UniProtKB:Q96DI7}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96DI7}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB22049.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK002371; BAB22049.1; ALT_FRAME; mRNA.
DR EMBL; AK169605; BAE41255.1; -; mRNA.
DR EMBL; BC058365; AAH58365.1; -; mRNA.
DR CCDS; CCDS38892.1; -.
DR RefSeq; NP_079921.2; NM_025645.2.
DR AlphaFoldDB; Q6PE01; -.
DR SMR; Q6PE01; -.
DR BioGRID; 211571; 18.
DR IntAct; Q6PE01; 2.
DR MINT; Q6PE01; -.
DR STRING; 10090.ENSMUSP00000101616; -.
DR iPTMnet; Q6PE01; -.
DR PhosphoSitePlus; Q6PE01; -.
DR EPD; Q6PE01; -.
DR MaxQB; Q6PE01; -.
DR PaxDb; Q6PE01; -.
DR PeptideAtlas; Q6PE01; -.
DR PRIDE; Q6PE01; -.
DR ProteomicsDB; 257279; -.
DR Antibodypedia; 16742; 131 antibodies from 17 providers.
DR DNASU; 66585; -.
DR Ensembl; ENSMUST00000105994; ENSMUSP00000101616; ENSMUSG00000074088.
DR GeneID; 66585; -.
DR KEGG; mmu:66585; -.
DR UCSC; uc008uzf.1; mouse.
DR CTD; 9410; -.
DR MGI; MGI:1913835; Snrnp40.
DR VEuPathDB; HostDB:ENSMUSG00000074088; -.
DR eggNOG; KOG0265; Eukaryota.
DR GeneTree; ENSGT00940000155058; -.
DR HOGENOM; CLU_000288_57_2_1; -.
DR InParanoid; Q6PE01; -.
DR OMA; WDLRKNA; -.
DR OrthoDB; 1133270at2759; -.
DR PhylomeDB; Q6PE01; -.
DR TreeFam; TF300039; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 66585; 29 hits in 75 CRISPR screens.
DR ChiTaRS; Snrnp40; mouse.
DR PRO; PR:Q6PE01; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q6PE01; protein.
DR Bgee; ENSMUSG00000074088; Expressed in hindlimb bud and 67 other tissues.
DR Genevisible; Q6PE01; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISO:MGI.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 5.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; Spliceosome; Ubl conjugation; WD repeat.
FT CHAIN 1..358
FT /note="U5 small nuclear ribonucleoprotein 40 kDa protein"
FT /id="PRO_0000051418"
FT REPEAT 65..104
FT /note="WD 1"
FT REPEAT 108..147
FT /note="WD 2"
FT REPEAT 150..190
FT /note="WD 3"
FT REPEAT 192..231
FT /note="WD 4"
FT REPEAT 234..273
FT /note="WD 5"
FT REPEAT 284..323
FT /note="WD 6"
FT REPEAT 326..358
FT /note="WD 7"
FT MOD_RES 21
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT CROSSLNK 18
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96DI7"
FT CROSSLNK 271
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96DI7"
FT CONFLICT 185
FT /note="K -> R (in Ref. 1; BAB22049)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="G -> D (in Ref. 1; BAB22049)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 358 AA; 39276 MW; 3B3EEA458D8228CA CRC64;
MIEQQKRKGP ELPLVPVKRP RHELLLGAAG AGPGAGPQQA TPGALLQAGP PRCSSLQAPI
MLLSGHEGEV YCCKFHPNGS TLASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT
DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP ARRGPQLVCT GSDDGTVKLW
DIRKKAAVQT FQNTYQVLAV TFNDTSDQII SGGIDNDIKV WDLRQNKLTY TMRGHADSVT
GLSLSSEGSY LLSNAMDNTV RVWDVRPFAP KERCVKIFQG NVHNFEKNLL RCSWSPDGSK
IAAGSADRFV YVWDTTSRRV LYKLPGHAGS INEVAFHPDE PIILSASSDK RLYMGEIQ
