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SNRPA_DROME
ID   SNRPA_DROME             Reviewed;         216 AA.
AC   P43332; Q9W4D7;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 180.
DE   RecName: Full=U1 small nuclear ribonucleoprotein A;
DE            Short=U1 snRNP A;
DE            Short=U1-A;
DE            Short=U1A;
DE   AltName: Full=Sex determination protein snf;
GN   Name=snf; Synonyms=D25, fs(1)1621, liz; ORFNames=CG4528;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1386424; DOI=10.1093/nar/20.14.3645;
RA   Harper D.S., Fresco L.D., Keene J.D.;
RT   "RNA binding specificity of a Drosophila snRNP protein that shares sequence
RT   homology with mammalian U1-A and U2-B' proteins.";
RL   Nucleic Acids Res. 20:3645-3650(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC   STRAIN=Oregon-R;
RX   PubMed=7926776; DOI=10.1101/gad.8.8.914;
RA   Flickinger T.W., Salz H.K.;
RT   "The Drosophila sex determination gene snf encodes a nuclear protein with
RT   sequence and functional similarity to the mammalian U1A snRNP protein.";
RL   Genes Dev. 8:914-925(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [6]
RP   INTERACTION WITH THE SMN COMPLEX.
RX   PubMed=18621711; DOI=10.1073/pnas.0802287105;
RA   Kroiss M., Schultz J., Wiesner J., Chari A., Sickmann A., Fischer U.;
RT   "Evolution of an RNP assembly system: a minimal SMN complex facilitates
RT   formation of UsnRNPs in Drosophila melanogaster.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10045-10050(2008).
CC   -!- FUNCTION: Binds stem loop II of U1 snRNA. It is the first snRNP to
CC       interact with pre-mRNA. This interaction is required for the subsequent
CC       binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a
CC       role in regulating sex-lethal splicing. {ECO:0000250,
CC       ECO:0000269|PubMed:7926776}.
CC   -!- SUBUNIT: Belongs to the spliceosome where it is associated with snRNP
CC       U1. Interacts with the SMN complex. {ECO:0000269|PubMed:18621711}.
CC   -!- INTERACTION:
CC       P43332; Q9V3N8: THG; NbExp=3; IntAct=EBI-174177, EBI-131989;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the RRM U1 A/B'' family. {ECO:0000305}.
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DR   EMBL; M89775; AAA28441.1; -; mRNA.
DR   EMBL; L29521; AAA28903.1; -; Genomic_DNA.
DR   EMBL; AE014298; AAF46017.1; -; Genomic_DNA.
DR   EMBL; AY061491; AAL29039.1; -; mRNA.
DR   PIR; A54279; A54279.
DR   RefSeq; NP_511045.1; NM_078490.4.
DR   PDB; 2AYM; NMR; -; A=134-216.
DR   PDB; 2B0G; NMR; -; A=134-216.
DR   PDB; 2K3K; NMR; -; A=1-98.
DR   PDB; 6F4G; X-ray; 1.90 A; B/E=1-96.
DR   PDB; 6F4H; X-ray; 2.00 A; A/C/E=1-96.
DR   PDB; 6F4I; X-ray; 1.49 A; A/B/C/D/E/F=1-96.
DR   PDB; 6F4J; X-ray; 1.42 A; C/D=1-96.
DR   PDBsum; 2AYM; -.
DR   PDBsum; 2B0G; -.
DR   PDBsum; 2K3K; -.
DR   PDBsum; 6F4G; -.
DR   PDBsum; 6F4H; -.
DR   PDBsum; 6F4I; -.
DR   PDBsum; 6F4J; -.
DR   AlphaFoldDB; P43332; -.
DR   BMRB; P43332; -.
DR   SMR; P43332; -.
DR   BioGRID; 57952; 40.
DR   DIP; DIP-23676N; -.
DR   IntAct; P43332; 4.
DR   STRING; 7227.FBpp0070716; -.
DR   PaxDb; P43332; -.
DR   PRIDE; P43332; -.
DR   DNASU; 31442; -.
DR   EnsemblMetazoa; FBtr0070748; FBpp0070716; FBgn0003449.
DR   GeneID; 31442; -.
DR   KEGG; dme:Dmel_CG4528; -.
DR   CTD; 31442; -.
DR   FlyBase; FBgn0003449; snf.
DR   VEuPathDB; VectorBase:FBgn0003449; -.
DR   eggNOG; KOG4206; Eukaryota.
DR   GeneTree; ENSGT00390000007046; -.
DR   HOGENOM; CLU_041869_1_1_1; -.
DR   InParanoid; P43332; -.
DR   OMA; NQTIYVN; -.
DR   OrthoDB; 1608132at2759; -.
DR   PhylomeDB; P43332; -.
DR   Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR   BioGRID-ORCS; 31442; 1 hit in 3 CRISPR screens.
DR   ChiTaRS; snf; fly.
DR   EvolutionaryTrace; P43332; -.
DR   GenomeRNAi; 31442; -.
DR   PRO; PR:P43332; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0003449; Expressed in eye disc (Drosophila) and 29 other tissues.
DR   ExpressionAtlas; P43332; baseline and differential.
DR   Genevisible; P43332; DM.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR   GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR   GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IDA:FlyBase.
DR   GO; GO:0030532; C:small nuclear ribonucleoprotein complex; IDA:FlyBase.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:FlyBase.
DR   GO; GO:0005685; C:U1 snRNP; ISS:FlyBase.
DR   GO; GO:0005686; C:U2 snRNP; ISS:FlyBase.
DR   GO; GO:0042802; F:identical protein binding; IPI:FlyBase.
DR   GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR   GO; GO:0008266; F:poly(U) RNA binding; IDA:FlyBase.
DR   GO; GO:0097158; F:pre-mRNA intronic pyrimidine-rich binding; IDA:FlyBase.
DR   GO; GO:0035614; F:snRNA stem-loop binding; IDA:FlyBase.
DR   GO; GO:0030619; F:U1 snRNA binding; IDA:FlyBase.
DR   GO; GO:0030620; F:U2 snRNA binding; IDA:FlyBase.
DR   GO; GO:0019099; P:female germ-line sex determination; NAS:FlyBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
DR   GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR   GO; GO:0007539; P:primary sex determination, soma; NAS:FlyBase.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IDA:FlyBase.
DR   GO; GO:0008380; P:RNA splicing; TAS:FlyBase.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   Pfam; PF00076; RRM_1; 2.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   1: Evidence at protein level;
KW   3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Ribonucleoprotein; RNA-binding; Spliceosome.
FT   CHAIN           1..216
FT                   /note="U1 small nuclear ribonucleoprotein A"
FT                   /id="PRO_0000081890"
FT   DOMAIN          7..86
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          142..216
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          97..142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        97..126
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        127..142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         49
FT                   /note="R -> H (in allele SNF1621; sterile)"
FT   STRAND          7..13
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   HELIX           20..31
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   TURN            32..34
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   STRAND          37..41
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   TURN            47..50
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   STRAND          52..58
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   HELIX           59..69
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   STRAND          73..78
FT                   /evidence="ECO:0007829|PDB:2K3K"
FT   STRAND          80..83
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   HELIX           87..94
FT                   /evidence="ECO:0007829|PDB:6F4J"
FT   STRAND          142..148
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   HELIX           155..162
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   STRAND          168..173
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   TURN            175..177
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   STRAND          179..186
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   HELIX           188..198
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   STRAND          201..204
FT                   /evidence="ECO:0007829|PDB:2AYM"
FT   STRAND          208..213
FT                   /evidence="ECO:0007829|PDB:2AYM"
SQ   SEQUENCE   216 AA;  24547 MW;  5B736FFE36523373 CRC64;
     MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG QAFVIFKEIG
     SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE RPKKVKPPKP APGTDEKKDK
     KKKPSSAENS NPNAQTEQPP NQILFLTNLP EETNEMMLSM LFNQFPGFKE VRLVPNRHDI
     AFVEFTTELQ SNAAKEALQG FKITPTHAMK ITFAKK
 
 
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